Thermo Scientific™

Pierce™ Fast Blocking Buffer

Catalog number:  37575

 Related applications:

Protein Assays and Analysis

| Protein Electrophoresis & Western Blotting

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Pierce Fast Blocking Buffer effectively and reliably blocks western blots in just five minutes to provide low-background results for traditional western blotting protocols.

Compare and view all available blocking buffers

Pierce Fast Blocking Buffer streamlines western blot protocols by providing consistent, high-quality blocking for nitrocellulose or PVDF membranes in only five minutes, producing results comparable to traditional blockers. Blocking and probing steps with Fast Blocking Buffer prevent nonspecific binding of detection antibodies during probing steps but allows specific detection to occur. Pierce Fast Wash Buffer provides efficient membrane washing after incubation with a primary and secondary antibody to effectively shorten total washing time to 20 minutes (from the typical 90 minutes). This buffer is compatible with antibodies and biotin-avidin systems, as well as both nitrocellulose and PVDF membranes.

Related products
Pierce Fast Wash Buffer, 10X

For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Product Line: Pierce™
Product Size: 500 mL
Reagent Type: Blocking Reagent
Validated Application: Western Blot

Contents & storage

Upon receipt store at 4°C.


Manuals & protocols