Thermo Scientific™

Pierce™ Fast Blocking Buffer

Catalog number:  37575

 Related applications:

Protein Assays and Analysis

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Thermo Scientific Pierce Fast Blocking Buffer effectively and reliably blocks western blots in just five minutes to provide low-background results and speed up traditional blotting protocols.

Features of Fast Blocking Buffer:

Fast—use both buffers to shorten the typical western blot development by over 2 hours
Simple—optimized protocol makes western blot analysis easier than ever
Low background—provides results comparable to classic western blotting buffers

Pierce Fast Blocking Buffer streamlines western blot protocols by providing consistent, high-quality blocking for nitrocellulose or PVDF membranes in only 5 minutes. Blocking and probing steps with Fast Blocking Buffer prevents nonspecific binding of detection antibodies during probing steps but allows specific detection to occur. Pierce Fast Wash Buffer provides efficient membrane washing after incubation with a primary and secondary antibody to effectively shorten total washing time to 20 minutes (from the typical 90 minutes). These two buffers are compatible with antibodies and biotin-avidin systems, as well as both nitrocellulose and PVDF membranes. The blocking buffer provides rapid and effective blocking in just five minutes while producing results comparable to traditional blockers for western blots.

Related Products
Pierce™ Fast Wash Buffer, 10X
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Product Line: Pierce™
Product Size: 500 mL
Reagent Type: Blocking Reagent
Validated Application: Western Blot

Contents & storage

Upon receipt store at 4°C.


Manuals & protocols