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Invitrogen
IRF8 Monoclonal Antibody (ZI003)
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FIGURE: 1 / 7
IRF8 Antibody (39-8800) in WB
Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of Raji (Lane 1), Ramos (Lane 2), K-562 (Lane 3), HL-60 (Lane 4), U-2 OS (Lane 5),THP-1 (Lane 6), NIH/3T3 (Lane 7) and T98G (Lane 8). The blot was probed with Anti- IRF8 Mouse Monoclonal Antibody (Product # 39-8800, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 48 kDa band corresponding to IRF8 was observed in Raji, Ramos and not observ... View More
Product Details
39-8800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
ZI003
Immunogen
Synthetic peptide derived from the C-terminal region of the human ICSBP protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H-ICSBP; ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8; unnamed protein product
Gene Aliases: AI893568; H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; IRF8; Myls
UniProt ID: (Human) Q02556, (Mouse) P23611
Entrez Gene ID: (Human) 3394, (Mouse) 15900
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
plasmacytoid dendritic cell differentiation
germinal center B cell differentiation
follicular B cell differentiation
immune system process
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
phagocytosis
autophagy
immune response
response to bacterium
myeloid cell differentiation
regulation of type I interferon production
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
defense response to bacterium
defense response to protozoan
positive regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to lipopolysaccharide
cellular response to interferon-gamma
dendritic cell differentiation
defense response to other organism
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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