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Invitrogen
ASK Polyclonal Antibody
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FIGURE: 1 / 1
ASK Antibody (40-7300) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), NTERA-2 (Lane 2), K-562 (Lane 3), HeLa (Lane 4) and Mouse testis (Lane 5). The blots were probed with Anti-ASK Rabbit Polyclonal Antibody (Product # 40-7300, 1-3 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 76 kDa band corresponding to ASK was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
40-7300
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the N-terminal region of the human ASK (DBF4, activator of S phase kinase) protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for ASK protein. On Western blots, it identifies the target band ~80 kDa.
Reactivity has been confirmed with human Farage, NTERA-2, Daudi, K562 and HeLa cell lysates.
Target Information
Activator of S phase kinase (ASK, DBF4) is a novel regulatory subunit of the human Cdc7 kinase complex that plays a pivotal role in G1/S cell cycle transition in mammalian cells. The increase in Cdc7 kinase activity at the G1/S boundary is at least partly accounted for by the elevated expression of ASK in late G1. ASK interacts with replication origins in vivo, suggesting that Cdc7 may trigger S phase by directly activating the replication initiation complexes assembled at the origins. Human ASK protein levels are regulated during the cell cycle with a pattern that matches that of human Cdc7 protein kinase activity. Human Cdc7-ASK kinase is directly involved in regulating the initiation of DNA replication by targeting MCM2 protein in mammalian cells. Nuclear localization and chromatin binding of endogenous huCdc7 and GFP-ASK is presumably imported into nuclei through its two nuclear localization signals at telophase, it may require additional signals for chromatin binding, the level of which increases at late G1 ohase. A functional link may exist between menin, a tumor suppressor protein mutated in multiple endocrine neoplasia type I (MEN1), and ASK in the regulation of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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