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Invitrogen
ErbB2 (HER-2) Monoclonal Antibody (MJD2), eFluor™ 570, eBioscience™
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FIGURE: 1 / 1
ErbB2 (HER-2) Antibody (41-9757-80) in ICC/IF
Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma tissue using 5 µg/mL of Anti-Human Epidermal Growth Factor Receptor-2 (ErbB2/HER-2) eFluor® 570. Nuclei are stained with DAPI (left). Immunocytochemistry of fixed and permeabilized BT474 cells using 5 µg/mL of Anti-Human Epidermal Growth Factor Receptor-2 (ErbB2/HER-2) eFluor® 570. Nuclei are stained with DAPI (righ... View More
Product Details
41-9757-80
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MJD2
Conjugate
Excitation/Emission Max
556/569 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Product Specific Information
Description: This MJD2 monoclonal antibody reacts with HER-2, the product of the ErbB2 gene and a member of the human epidermal growth factor receptor (EGFR) family. HER-2 is a 185 kDa transmembrane receptor with an extracellular binding domain and an intracellular tyrosine kinase domain. Activation of HER-2 occurs following formation of homodimers or heterodimers with ligand-bound EGFR family members, HER3 or HER4. Overexpression of HER-2 results in auto-phosphorylation and increased proliferation and invasiveness and is associated with subsets of multiple cancer types such as breast, ovarian, cervical, uterine, and gastric. The use of HER-2 targeted agents has been used extensively to treat HER-2-positive breast cancer, but a recent study found that these agents can also block the proliferation of breast cancer stem cells found in HER-2-negative patients and suggests the importance of HER-2 as a target for multiple types of breast cancer. The MJD2 antibody recognizes the extracellular domain of HER-2 but at an epitope distinct from that recognized by the 4D5 (Herceptin) and SP3 monoclonal antibodies.
Applications Reported: This MJD2 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This MJD2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval at less than or equal to 5 µg/mL. This MJD2 antibody has been tested by immunocytochemistry of methanol-fixed human cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.
Excitation: 555 nm; Emission: 570 nm
Target Information
ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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