Insulin-Transferrin-Selenium (ITS -G) (100X)

Try new Gibco Insulin-Transferrin-Selenium Select supplements, an animal-origin free formulation. Available in ITS-G & ITS-X. Learn more >

Insulin-Transferrin-Selenium (ITS -G) (100X)

Citations & References (4)

Gibco™

Insulin-Transferrin-Selenium (ITS -G) (100X)

Gibco™インスリン-トランスフェリン-セレン（ITS-G）は、細胞培養に必要なFBSの量を低減するための基礎培地サプリメントとして使用されます。ITS-G溶液は、当初はRPMI 1640および最小必須培地（MEM）で使用されていましたが、現在では詳細を見る

製品番号（カタログ番号）	数量
41400045	10 mL

製品番号（カタログ番号） 41400045

価格（JPY）

6,200

お問い合わせください ›

10 mL

Gibco™インスリン-トランスフェリン-セレン（ITS-G）は、細胞培養に必要なFBSの量を低減するための基礎培地サプリメントとして使用されます。ITS-G溶液は、当初はRPMI 1640および最小必須培地（MEM）で使用されていましたが、現在では、他の基礎培地とともに日常的に使用されて、2–4%FBSの存在下でさまざまな細胞タイプをサポートしています

インスリンは、グルコースやアミノ酸の取り込み、リポゲネシス、細胞内輸送、タンパク質や核酸の合成を促進しますトランスフェリンは鉄のキャリアであり、酸素ラジカルおよび過酸化物の毒性レベルを低減する役に立つ場合もあります。セレナイトナトリウムとしてのセレンは、グルタチオンペルオキシダーゼや他のタンパク質の補助因子であり、培地中の抗酸化剤として使用されます

詳細な組成については、こちらをご覧ください。

cGMP製造および品質システム
Gibco™ ITS-Gは、ニューヨーク州グランドアイランドにあるcGMP準拠施設で製造されています。この施設は医療機器製造業者としてFDAに登録されており、ISO 13485の認証を受けています。

研究用にのみ使用できます。診断用には使用いただけません。

細胞タイプMammalian Cells

濃度100 X

数量10 mL

品質保持期間18 Months

出荷条件Room Temperature

形状Liquid

製品タイプTransferrin

無菌性Sterile-filtered

添加剤なしNo Phenol Red

Unit SizeEach

組成および保存条件

Storage conditions: 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 18 months from date of manufacture

よくあるご質問（FAQ）

What is the buffer composition of the various Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

All ITS and ITS Select supplements are formulated in Earle's Balanced Salt Solution (EBSS).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

ITS Select supplements are animal origin-free (AOF) versions of the ITS supplements offered by Thermo Fisher Scientific.

ITS Select supplements use recombinant versions of both proteins in the formulation: insulin and transferrin.

All other versions of ITS, those without the Select designation, utilize recombinant insulin and a human-derived holo transferrin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the individual concentrations of insulin, transferrin, and selenium in the Insulin-Transferrin-Selenium (ITS) (100X) supplements?

All ITS and ITS Select supplements have the following concentrations of their components:

Insulin: 1.0 g/L
Transferrin: 0.55 g/L
Selenium: 0.00067 g/L

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store aliquots of Insulin-Transferrin-Selenium (ITS) (100X) supplements at -20 degrees C without compromising the integrity of the product?

We recommend storing all ITS and ITS Select supplements at 2-8 degrees C as there is a chance that the insulin will precipitate upon freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are all Insulin-Transferrin-Selenium (ITS) (100X) supplements offered by Thermo Fisher Scientific animal origin-free?

We offer both animal origin and animal origin-free versions of our ITS supplements. The animal origin-free product versions are referred to as ITS Select supplements.

Insulin-Transferrin-Selenium (ITS-G) (100X) (Cat. No. 41400045), Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (100X) (Cat. No. 51500056), and Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Cat. No. 51300044) are not animal origin-free.

Animal origin-free product versions are available for the ITS-G and ITS-X formulations: Insulin-Transferrin-Selenium (ITS-G) Select Supplement (100X) (Cat. No. A4000046501 and A4000046502) and Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) Select Supplement (100X) (Cat. No. A4000046401 and Cat. No. A4000046402.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Insulin-Transferrin-Selenium (ITS -G) (100X) FAQs

引用および参考文献 (4)

引用および参考文献

Abstract

A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.

Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T

Journal:Experimental cell research

PubMed ID:38499143

Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.

Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA

Journal:STAR protocols

PubMed ID:38833372

Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic âlivers in a dish.â We describe strategies to induce ... More

Collective cell migration requires suppression of actomyosin at cell-cell contacts mediated by DDR1 and the cell polarity regulators Par3 and Par6.

Authors:Hidalgo-Carcedo C, Hooper S, Chaudhry SI, Williamson P, Harrington K, Leitinger B, Sahai E,

Journal:Nat Cell Biol

PubMed ID:21170030

Collective cell migration occurs in a range of contexts: cancer cells frequently invade in cohorts while retaining cell-cell junctions. Here we show that collective invasion by cancer cells depends on decreasing actomyosin contractility at sites of cell-cell contact. When actomyosin is not downregulated at cell-cell contacts, migrating cells lose cohesion. ... More

Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology.

Authors:Smits AM, van Vliet P, Metz CH, Korfage T, Sluijter JP, Doevendans PA, Goumans MJ,

Journal:Nat Protoc

PubMed ID:19197267

To date, there is no suitable in vitro model to study human adult cardiac cell biology. Although embryonic stem cells are able to differentiate into cardiomyocytes in vitro, the efficiency of this process is very low. Other methods to differentiate progenitor cells into beating cardiomyocytes rely on coculturing with rat ... More