Insulin-Transferrin-Selenium (ITS -G) (100X) - FAQs

All ITS and ITS Select supplements are formulated in Earle's Balanced Salt Solution (EBSS).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

ITS Select supplements are animal origin-free (AOF) versions of the ITS supplements offered by Thermo Fisher Scientific.

ITS Select supplements use recombinant versions of both proteins in the formulation: insulin and transferrin.

All other versions of ITS, those without the Select designation, utilize recombinant insulin and a human-derived holo transferrin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

All ITS and ITS Select supplements have the following concentrations of their components:

Insulin: 1.0 g/L
Transferrin: 0.55 g/L
Selenium: 0.00067 g/L

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend storing all ITS and ITS Select supplements at 2-8 degrees C as there is a chance that the insulin will precipitate upon freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We offer both animal origin and animal origin-free versions of our ITS supplements. The animal origin-free product versions are referred to as ITS Select supplements.

Insulin-Transferrin-Selenium (ITS-G) (100X) (Cat. No. 41400045), Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (100X) (Cat. No. 51500056), and Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Cat. No. 51300044) are not animal origin-free.

Animal origin-free product versions are available for the ITS-G and ITS-X formulations: Insulin-Transferrin-Selenium (ITS-G) Select Supplement (100X) (Cat. No. A4000046501 and A4000046502) and Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) Select Supplement (100X) (Cat. No. A4000046401 and Cat. No. A4000046402.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

All ITS and ITS Select supplements have a shelf life of between 12 to 18 months from date of manufacture when stored at 2-8 degrees C (see product pages for specific shelf-life). In general, we do not recommend storing these products at -20 degrees C due to the potential issue of proteins precipitating from the solution upon thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Our ITS supplements contain human-derived holo transferrin and our ITS Select supplements contain a partially saturated recombinant human transferrin produced in a plant-based expression system, that performs similarly to human-derived holo transferrin in cell culture applications. This information can also be accessed in the Certificate of Origin (COO).

The following ITS supplements use human-derived holo transferrin: Insulin-Transferrin-Selenium (ITS-G) (100X) (Cat. No. 41400045), Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (100X) (Cat. No. 51500056), and Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Cat. No. 51300044).

The following ITS Select supplements use recombinant human transferrin produced in a plant-based expression system: Insulin-Transferrin-Selenium (ITS-G) Select Supplement (100X) (Cat. No. A4000046501 and A4000046502) and Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) Select Supplement (Cat. No. A4000046401 and Cat. No. A4000046402).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Recombinant human insulin is used in all ITS and ITS Select supplements.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

All ITS and ITS Select supplements are provided at 100X concentration. These can be added to media at 10mL per 1L of media. This can reduce the amount of FBS required (typically to concentrations of 2- 4%).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

ITS-G and ITS-G Select supplements have been designed to supplement basal medium to support the growth of various cell types at reduced FBS concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

ITS and ITS Select supplements are used to reduce the amount of fetal bovine serum (FBS) required for routine maintenance and low-density attachment of many cell types. They may also be used for supplementation of lab-developed media formulations.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

ITS and ITS Select supplements contain insulin, transferrin, and sodium selenite. In addition to these components, ITS-X and ITS-X Select supplements contain ethanolamine and ITS-A supplement contains sodium pyruvate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.