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          • Primary Antibodies ›
          • PPAR gamma Antibodies

          Invitrogen

          PPAR gamma Monoclonal Antibody (A3409A)

          Advanced Verification
          1 Published Figure
          4 References
          View all (25) PPAR gamma antibodies

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          Cite PPAR gamma Monoclonal Antibody (A3409A)

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          • Antibody Testing Data (4)
          • Published Figures (1)
          • Advanced Verification (1)
          PPAR gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          PPAR gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          PPAR gamma Antibody (419300) in IHC (P)

          Immunohistochemistry analysis of PPAR gamma in paraffin-embedded rat adipose tissue. The sample was incubated in a PPAR gamma monoclonal antibody (Product # 419300) at a dilution of 20 µg/mL. Section was fixed with 10% formalin-fixed in immersion, activated by autoclave at 121°C for 15 minutes, and blocked with 3% H2O2 in methanol. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          PPAR gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PPAR gamma Antibody in Western Blot (WB)
          PPAR gamma Antibody in Western Blot (WB)
          PPAR gamma Antibody in Western Blot (WB)
          PPAR gamma Antibody in Immunohistochemistry (Frozen) (IHC (F))
          PPAR gamma Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          PPAR gamma Monoclonal Antibody (A3409A)

          Product Details

          419300

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          10 µg/mL
          -

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          12 ng/mL
          -

          Immunoprecipitation (IP)

          Assay-dependent
          View 1 publication 1 publication

          ChIP assay (ChIP)

          Assay-dependent
          -

          Gel Shift (EMSA)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Mouse, Rat

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          A3409A

          Immunogen

          Baculovirus-expressed recombinant human PPAR-gamma-1 (3-108 aa).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Ammonium sulfate precipitation

          Storage buffer

          physiological saline

          Contains

          0.1% sodium azide

          Storage conditions

          Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2532203

          Product Specific Information

          This antibody specifically recognizes human PPAR gamma1 and 2 and cross reacts with mouse and rat PPAR gamma1 and 2. This antibody does not recognize human PPAR alpha and delta.

          Target Information

          This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: HUMPPARG; ligand-activated nuclear transcription factor; ligand-activated transcription factor; nuclear receptor; nuclear receptor family member; Pparg; nuclear receptor subfamily 1 group C member 3; OTTHUMP00000185032; OTTHUMP00000185036; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator activator receptor, gamma; peroxisome proliferator-activated receptor-gamma 5; peroxisome proliferator-activated receptor-gamma splicing; PPAR gamma; PPAR-gamma; PPAR-gamma-1a; PPAR-gamma-1b; Pparg; PPARy; unnamed protein product

          View more View less

          Gene Aliases: CIMT1; FPLD3; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG1; PPARG2; PPARG5; PPARgamma; PPARgamma2

          View more View less

          UniProt ID: (Human) P37231, (Rat) O88275, (Mouse) P37238

          View more View less

          Entrez Gene ID: (Human) 5468, (Rat) 25664, (Mouse) 19016

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding nucleic acid binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding prostaglandin receptor activity protein binding zinc ion binding enzyme binding peptide binding identical protein binding sequence-specific DNA binding retinoid X receptor binding arachidonic acid binding DBD domain binding LBD domain binding WW domain binding alpha-actinin binding R-SMAD binding E-box binding STAT family protein binding DNA-binding transcription factor binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription coactivator binding protein phosphatase binding estrogen receptor binding promoter-specific chromatin binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter negative regulation of cytokine production placenta development response to dietary excess diet induced thermogenesis wound healing involved in inflammatory response negative regulation of acute inflammatory response regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter fatty acid metabolic process response to stress heart development regulation of blood pressure negative regulation of cell proliferation response to xenobiotic stimulus response to light stimulus response to mechanical stimulus hormone-mediated signaling pathway regulation of gene expression negative regulation of cardiac muscle hypertrophy positive regulation of gene expression negative regulation of gene expression negative regulation of macrophage derived foam cell differentiation positive regulation of cholesterol efflux negative regulation of cholesterol storage negative regulation of lipid storage negative regulation of smooth muscle cell migration long-chain fatty acid transport negative regulation of angiogenesis regulation of lipid metabolic process fatty acid oxidation cell differentiation monocyte differentiation negative regulation of cell growth BMP signaling pathway negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway intracellular receptor signaling pathway epithelial cell differentiation response to caffeine organ regeneration positive regulation of prostaglandin biosynthetic process response to nutrient levels response to food positive regulation of cholesterol transport positive regulation of intracellular cholesterol transport cellular response to insulin stimulus cellular response to hormone stimulus negative regulation of collagen biosynthetic process response to vitamin A response to lipid peroxisome proliferator activated receptor signaling pathway response to immobilization stress cellular response to platelet-derived growth factor stimulus glucose homeostasis response to starvation regulation of circadian rhythm mRNA transcription from RNA polymerase II promoter positive regulation of apoptotic process negative regulation of MAPK cascade negative regulation of blood vessel endothelial cell migration response to estrogen cell fate commitment fat cell differentiation regulation of fat cell differentiation positive regulation of fat cell differentiation negative regulation of osteoblast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of fatty acid metabolic process positive regulation of transcription from RNA polymerase II promoter positive regulation of fatty acid oxidation regulation of hormone secretion retinoic acid receptor signaling pathway cell maturation negative regulation of smooth muscle cell proliferation positive regulation of oligodendrocyte differentiation positive regulation of protein secretion negative regulation of inflammatory response white fat cell differentiation brown fat cell differentiation positive regulation of phagocytosis, engulfment negative regulation of interferon-gamma-mediated signaling pathway positive regulation of SMAD protein import into nucleus negative regulation of SMAD protein import into nucleus positive regulation of adiponectin secretion response to linoleic acid cellular response to lithium ion cellular response to retinoic acid cellular response to vitamin E cellular response to prostaglandin stimulus cellular response to prostaglandin E stimulus cellular response to low-density lipoprotein particle stimulus cellular response to hyperoxia cellular response to hypoxia negative regulation of mitochondrial fission negative regulation of peptide hormone secretion cold-induced thermogenesis positive regulation of lipoprotein transport beige fat cell differentiation negative regulation of cellular response to insulin stimulus negative regulation of extracellular matrix assembly negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of protein localization to plasma membrane negative regulation of cardiac muscle hypertrophy in response to stress negative regulation of cellular response to transforming growth factor beta stimulus positive regulation of adipose tissue development negative regulation of connective tissue replacement involved in inflammatory response wound healing negative regulation of vascular smooth muscle cell proliferation negative regulation of receptor signaling pathway via STAT positive regulation of vascular associated smooth muscle cell apoptotic process negative regulation of vascular endothelial cell proliferation negative regulation of pancreatic stellate cell proliferation positive regulation of apoptotic signaling pathway G-protein coupled receptor signaling pathway response to nutrient innate immune response positive regulation of lipid metabolic process regulation of transport negative regulation of multicellular organismal process lipid homeostasis nuclear receptor-mediated signaling pathway regulation of cellular response to insulin stimulus inflammatory response
          It has to be done as per old AB suggested Products section.

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