MicroSEQ™ Full Gene 16S rDNA PCR Kit - FAQs

The MicroSEQ Full Gene 16S rDNA PCR Kit includes universal domain-level primers that are designed to amplify the bacterial 16S rRNA gene.

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A 60-day-free trial of the MicrobeBridge Software can be downloaded here: www.thermofisher.com/order/catalog/product/A29238

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For the MicrobeBridge Software, please contact your local Thermo Fisher Scientific Technical Support (https://www.thermofisher.com/us/en/home/technical-resources/contact-us.html) or email abtechsupport@thermofisher.com.

For MicrobeNet, please contact the CDC directly at microbenet@cdc.gov. This account is actively monitored by the CDC MicrobeNet staff.

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The MicrobeBridge Software has the following recommended hardware requirements: Minimum 1.8 GHz processor, 2 GB RAM, and 20 GB of free hard disk space. MicrobeBridge Software has been tested on Windows 7 32-bit SP1, Windows 7 64-bit SP1, and Windows 10 IoT.

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No data is lost with the MicrobeBridge Software installation of the full version on top of the free trial version.

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Only .ab1 files can be used with the MicrobeBridge Software.

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The MicrobeBridge Software fully supports data outputs from Applied Biosystems 3500, 3500xL, 3130, 3130xl, and SeqStudio genetic analyzer systems.

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To use MicrobeNet, you will need to register for a MicrobeNet account. You can register at https://microbenet.cdc.gov/

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Yes, MicrobeNet database provides species-level identification.

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MicrobeBridge Software does not do bacterial identification. It provides a connection to MicrobeNet which does the bacterial identification.

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No. MicrobeBridge Software does not provide any search parameters which are used by MicrobeNet software.

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Yes, you can edit the contig sequence generated by the MicrobeBridge software.

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Yes, the final contig sequence generated by MicrobeBridge Software can be exported for use in other database searches.

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Yes, MicrobeBridge Software uses Applied Biosystems KB Basecaller v1.4.2.4, by default.

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As long as the 16S full gene is covered, a specific chemistry is not needed when using the MicrobeBridge Software.

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CDC MicrobeNet has recommended primers which have been configured into the MicrobeBridge Software. We recommend using MicroSEQ 16S Full Gene kit for a complete package solution.

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Data generated from the MicroSEQ 16S Full Gene Kit package can be analyzed using MicrobeBridge Software.

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Through a partnership with the Special Bacteriology Reference Laboratory at the CDC, Thermo Fisher Scientific developed the MicrobeBridge Software to enable public health, microbiology, and any other laboratories worldwide to directly access the CDC's MicrobeNet virtual reference database using 16S rRNA sequencing technology.

MicrobeBridge Software provides a streamlined workflow for analyzing Sanger sequencing data (.ab1 data files) generated on CE Sanger sequencing instruments or genetic analyzers. MicrobeBridge Software performs contig assembly, contig editing, primer trimming, data quality check, contig sequence copying, and one-click connection to MicrobeNet for bacterial identification through 16S rRNA BLAST searches.

In contrast, MicroSEQ ID software is part of a complete system designed for pharmaceutical analytics workflows. The system includes secondary analysis software, validated reference libraries for bacterial and fungal identification, links to query public domain databases, creation of custom reference databases, and SAE features that enables 21 CFR Part 11 compliance.

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MicrobeBridge Software is not a replacement for the MicroSEQ ID software. MicrobeBridge Software is specifically designed for public health, microbiology, and any other laboratories utilizing the MicrobeNet database. Applied Biosystems MicroSEQ ID Microbial Identification software is designed for use in pharmaceutical analytics laboratories who typically do not use the MicrobeNet database but require other functionalities. However MicroSEQ ID users can install and use MicrobeBridge Software if they wish to do MicrobeNet searches.

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Public health, microbiology, and any other laboratories conducting disease surveillance or bacterial identification with MicrobeNet can benefit from using the MicrobeBridge Software.

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Some of the benefits of using the MicrobeBridge Software Faster are faster turnaround time and lower cost:

Using MicrobeBridge Software, MicrobeNet users are able to efficiently assemble and QC their sequencing data using the CDC recommended primer set for 16S rRNA gene sequencing analysis. Users are able to get to their results quickly and accurately, thereby saving money and time spent assembling, interrogating, and identifying bacteria or pathogens in their data.

Users can connect to MicrobeNet via a single click in the software or export the consensus contig and this in turn improves the bacterial identification workflows in public health and microbiology laboratories using MicrobeNet.

Users are able to connect directly into the CDC MicrobeNet virtual reference database for identifying rare and complex pathogens faster and more accurately. Although any laboratory with an internet connection can access the MicrobeNet database worldwide, MicrobeBridge Software streamlines the CE data and connects to MicrobeNet directly.

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The Applied Biosystems MicrobeBridge Software is a desktop application which connects .ab1 data files generated on Applied Biosystems 3500 and 3130 Genetic Analyzers and 3730 DNA Analyzers with the Centers for Disease Control and Prevention (CDC) MicrobeNet virtual reference database via internet connection for bacterial identification using 16S rRNA gene sequencing analysis.

MicrobeNet is a web-based reference database for the identification of microbial pathogens through 16S rRNA gene sequence BLAST searches. MicrobeNet contains a database of genetic and phenotypic properties for bacterial identification actively curated by the CDC.

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