Characterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 and their inhibitors in equine granulosa cells in vivo and in vitro
AuthorsSessions, DR; Vick, MM; Fitzgerald, BP
JournalJOURNAL OF ANIMAL SCIENCE
PubMed ID
Matrix metalloproteinases (MMP) and tissue inhibitors of MMP (TIMP) regulate tissue remodeling events necessary for ovulation. Thus, changes in MMP and TIMP expression and protein enzyme activity were examined in vivo and in vitro during follicular development and atresia in the horse. Equine granulosa cells and follicular fluid from medium ... More
Molecular cloning and characterization of porcine calcineurin-alpha subunit expression in skeletal muscle.
AuthorsDepreux FF, Scheffler JM, Grant AL, Bidwell CA, Gerrard DE
JournalJ Anim Sci
PubMed ID19897633
The calmodulin/Ca(2+)-dependent serine/threonine phophatase, calcineurin (CaN), has been implicated in controlling muscle fiber phenotype. However, little information is available concerning the expression of CaN in porcine skeletal muscle. Therefore, the porcine CaN alpha (CaN-A) was cloned by reverse transcription-PCR and its expression characterized in selected porcine skeletal muscles. We successfully ... More
Cell lines as candidate reference materials for quality control of ERBB2 amplification and expression assays in breast cancer.
AuthorsXiao Y, Gao X, Maragh S, Telford WG, Tona A
JournalClin Chem
PubMed ID19443566
BACKGROUND: Human epidermal growth factor receptor 2 (HER2) is an important biomarker whose status plays a pivotal role in therapeutic decision-making for breast cancer patients and in determining their clinical outcomes. Ensuring the accuracy and reproducibility of HER2 assays by immunohistochemistry (IHC) and by fluorescence in situ hybridization (FISH) ... More
Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro.
AuthorsSugiyama K, Suzuki K, Nakazawa T, Funami K, Hishiki T, Ogawa K, Saito S, Shimotohno KW, Suzuki T, Shimizu Y, Tobita R, Hijikata M, Takaku H, Shimotohno K
JournalJ Virol
PubMed ID19369330
Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5' untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, ... More
HIV-1 transactivator protein induction of suppressor of cytokine signaling-2 contributes to dysregulation of IFN{gamma} signaling.
AuthorsCheng SM, Li JC, Lin SS, Lee DC, Liu L, Chen Z, Lau AS
JournalBlood
PubMed ID19279332
HIV infection remains a worldwide threat. HIV-1 transactivator protein Tat is one of the retroviral proteins identified as a key immunomodulator in AIDS pathogenesis. Although the primary function of Tat is to regulate HIV-1 replication in the infected cell, it also dysregulates cytokine production resulting in perturbation of the host ... More
PURPOSE: The purpose of this study was to determine the role of hepatocyte growth factor (HGF) and c-Met in the initiation and development of retinal neovascularization and to determine whether inhibition of this system can suppress the extent of angiogenesis in an animal model. METHODS: Retinal tissues from animals ... More
Dynamic PolyConjugates for targeted in vivo delivery of siRNA to hepatocytes.
AuthorsRozema DB, Lewis DL, Wakefield DH, Wong SC, Klein JJ, Roesch PL, Bertin SL, Reppen TW, Chu Q, Blokhin AV, Hagstrom JE, Wolff JA
JournalProc Natl Acad Sci U S A
PubMed ID17652171
Achieving efficient in vivo delivery of siRNA to the appropriate target cell would be a major advance in the use of RNAi in gene function studies and as a therapeutic modality. Hepatocytes, the key parenchymal cells of the liver, are a particularly attractive target cell type for siRNA delivery ... More
A novel one-armed anti-c-Met antibody inhibits glioblastoma growth in vivo.
AuthorsMartens T, Schmidt NO, Eckerich C, Fillbrandt R, Merchant M, Schwall R, Westphal M, Lamszus K
JournalClin Cancer Res
PubMed ID17062691
PURPOSE: Expression of the receptor tyrosine kinase c-Met and its ligand scatter factor/hepatocyte growth factor (SF/HGF) are strongly increased in glioblastomas, where they promote tumor proliferation, migration, invasion, and angiogenesis. We used a novel one-armed anti-c-Met antibody to inhibit glioblastoma growth in vivo. EXPERIMENTAL DESIGN: U87 glioblastoma cells (c-Met ... More
Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina
Infection with Equid Herpesvirus type 1 (EHV-1) leads to respiratory disease, abortion, and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A(2254)/G(2254)) in the genome region of the open reading frame 30 (ORF30), which results in an amino acid variation (N(752)/D(752)) of the EHV-1 ... More
Breed difference and regulation of the porcine adipose triglyceride lipase and hormone sensitive lipase by TNFα
AuthorsShan, T; Wu, T; Reng, Y; Wang, Y
JournalANIMAL GENETICS
PubMed ID
Adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) are major novel triglyceride lipases in animals. The aim of this study was to determine if there are differences in the porcine ATGL (pATGL) and HSL genes between Jinhua pigs (a fatty breed) and Landrace pigs (a leaner breed). In addition, ... More
Expression profiling and electrophysiological studies suggest a major role for Orai1 in the store-operated Ca(2+) influx pathway of platelets and megakaryocytes
Store-operated Ca(2+) influx represents a major route by which cytosolic Ca(2+) can be elevated during platelet activation, yet its molecular identity in this cell type remains highly controversial. Using quantitative RT-PCR analysis of candidate receptor-operated cation entry pathways in human platelets, we show a 30-fold higher expression of message for ... More
Targeting the mRNA-binding protein HuR impairs malignant characteristics of pancreatic ductal adenocarcinoma cells.
Post-transcriptional regulation is a powerful mediator of gene expression, and can rapidly alter the expression of numerous transcripts involved in tumorigenesis. We have previously shown that the mRNA-binding protein HuR (ELAVL1) is elevated in human pancreatic ductal adenocarcinoma (PDA) specimens compared to normal pancreatic tissues, and its cytoplasmic localization is ... More
Seven-valent pneumococcal conjugate vaccine and nasopharyngeal microbiota in healthy children.
AuthorsBiesbroek G,Wang X,Keijser BJ,Eijkemans RM,Trzciński K,Rots NY,Veenhoven RH,Sanders EA,Bogaert D
JournalEmerging infectious diseases
PubMed ID24447437
Seven-valent pneumococcal conjugate vaccine (PCV-7) is effective against vaccine serotype disease and carriage. Nevertheless, shifts in colonization and disease toward nonvaccine serotypes and other potential pathogens have been described. To understand the extent of these shifts, we analyzed nasopharyngeal microbial profiles of 97 PCV-7âvaccinated infants and 103 control infants participating ... More
Variability and diversity of nasopharyngeal microbiota in children: a metagenomic analysis.
The nasopharynx is the ecological niche for many commensal bacteria and for potential respiratory or invasive pathogens like Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis. Disturbance of a balanced nasopharyngeal (NP) microbiome might be involved in the onset of symptomatic infections with these pathogens, which occurs primarily in fall and ... More
Deep sequencing analyses of low density microbial communities: working at the boundary of accurate microbiota detection.
INTRODUCTION: Accurate analyses of microbiota composition of low-density communities (10(3)â10(4) bacteria/sample) can be challenging. Background DNA from chemicals and consumables, extraction biases as well as differences in PCR efficiency can significantly interfere with microbiota assessment. This study was aiming to establish protocols for accurate microbiota analysis at low microbial density. ... More
Elevated paternal glucocorticoid exposure alters the small noncoding RNA profile in sperm and modifies anxiety and depressive phenotypes in the offspring.
AuthorsShort AK,Fennell KA,Perreau VM,Fox A,O'Bryan MK,Kim JH,Bredy TW,Pang TY,Hannan AJ
JournalTranslational psychiatry
PubMed ID27300263
Recent studies have suggested that physiological and behavioral traits may be transgenerationally inherited through the paternal lineage, possibly via non-genomic signals derived from the sperm. To investigate how paternal stress might influence offspring behavioral phenotypes, a model of hypothalamicâpituitaryâadrenal (HPA) axis dysregulation was used. Male breeders were administered water supplemented ... More
The adult nasopharyngeal microbiome as a determinant of pneumococcal acquisition.
BACKGROUND: Several cohort studies have indicated associations between S. pneumoniae and other microbes in the nasopharynx. To study causal relationships between the nasopharyngeal microbiome and pneumococcal carriage, we employed an experimental human pneumococcal carriage model. Healthy adult volunteers were assessed for pneumococcal carriage by culture of nasal wash samples (NWS). ... More
GCN5 Regulates FGF Signaling and Activates Selective MYC Target Genes during Early Embryoid Body Differentiation.
Precise control of gene expression during development is orchestrated by transcription factors and co-regulators including chromatin modifiers. How particular chromatin-modifying enzymes affect specific developmental processes is not well defined. Here, we report that GCN5, a histone acetyltransferase essential for embryonic development, is required for proper expression of multiple genes encoding ... More
Placenta-Enriched LincRNAs MIR503HG and LINC00629 Decrease Migration and Invasion Potential of JEG-3 Cell Line.
AuthorsMuys BR,Lorenzi JC,Zanette DL,Lima e Bueno Rde B,de Araújo LF,Dinarte-Santos AR,Alves CP,Ramão A,de Molfetta GA,Vidal DO,Silva WA Jr
JournalPloS one
PubMed ID27023770
LINC00629 and MIR503HG are long intergenic non-coding RNAs (lincRNAs) mapped on chromosome X (Xq26), a region enriched for genes associated with human reproduction. Genes highly expressed in normal reproductive tissues and cancers (CT genes) are well known as potential tumor biomarkers. This study aimed to characterize the structure, expression, function ... More
Inducing gene expression by targeting promoter sequences using small activating RNAs.
Vector-based systems comprised of exogenous nucleic acid sequences remain the standard for ectopic expression of a particular gene. Such systems offer robust overexpression, but have inherent drawbacks such as the tedious process of construction, excluding sequences (e.g. introns and untranslated regions) important for gene function and potential insertional mutagenesis of ... More
Voltage-dependent Ca(2+) entry into smooth muscle during contraction promotes endothelium-mediated feedback vasodilation in arterioles.
AuthorsGarland CJ,Bagher P,Powell C,Ye X,Lemmey HAL,Borysova L,Dora KA
JournalScience signaling
PubMed ID28676489
Tumor-Associated Macrophage Promotes the Survival of Cancer Cells upon Docetaxel Chemotherapy via the CSF1/CSF1R-CXCL12/CXCR4 Axis in Castration-Resistant Prostate Cancer.
AuthorsGuan W,Li F,Zhao Z,Zhang Z,Hu J,Zhang Y
JournalGenes
PubMed ID34069563
Castration-resistant prostate cancer (CRPC) is an advanced stage of prostate cancer that can progress rapidly even in patients treated with castration. Previously, we found that tumor-associated macrophages (TAM) can be recruited by CSF-1 secreted by docetaxel-treated prostate cancer cells and promote the survival of cancer cells in response to chemotherapy. ... More
Genomic profiling of NGS-based ctDNA from Chinese non-small cell lung cancer patients.
JournalJournal of cancer research and clinical oncology
PubMed ID37186065
Cost-effective high-throughput single-haplotype iterative mapping and sequencing for complex genomic structures.
AuthorsBellott DW,Cho TJ,Hughes JF,Skaletsky H,Page DC
JournalNature protocols
PubMed ID29565902
The reference sequence of structurally complex regions can only be obtained through highly accurate clone-based approaches. We and others have successfully employed Single-Haplotype Iterative Mapping and Sequencing (SHIMS 1.0) to assemble structurally complex regions across the sex chromosomes of several vertebrate species and in targeted improvements to the reference sequences ... More
Characterizing the Transcriptional Effects of Endolysin Treatment on Established Biofilms of Staphylococcus aureus.
AuthorsFernández L,González S,Gutiérrez D,Campelo AB,Martínez B,Rodríguez A,García P
JournalBio-protocol
PubMed ID34286000
Biofilms are the most common lifestyle of bacteria in both natural and human environments. The organized structure of these multicellular communities generally protects bacterial cells from external challenges, thereby enhancing their ability to survive treatment with antibiotics or disinfectants. For this reason, the search for new antibiofilm strategies is an ... More
Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis.
AuthorsHiroyasu A,DeWitt DC,Goodman AG
JournalJournal of visualized experiments : JoVE
PubMed ID29889203
During the pathogenic infection of Drosophila melanogaster, hemocytes play an important role in the immune response throughout the infection. Thus, the goal of this protocol is to develop a method to visualize the pathogen invasion in a specific immune compartment of flies, namely hemocytes. Using the method presented here, up ... More
Genetics and Genomic Regions Affecting Response to Newcastle Disease Virus Infection under Heat Stress in Layer Chickens.
AuthorsSaelao P,Wang Y,Chanthavixay G,Gallardo RA,Wolc A,Dekkers JCM,Lamont SJ,Kelly T,Zhou H
JournalGenes
PubMed ID30669351
Newcastle disease virus (NDV) is a highly contagious avian pathogen that poses a tremendous threat to poultry producers in endemic zones due to its epidemic potential. To investigate host genetic resistance to NDV while under the effects of heat stress, a genome-wide association study (GWAS) was performed on Hy-Line Brown ... More
METTL14 drives growth and metastasis of non-small cell lung cancer by regulating pri-miR-93-5p maturation and TXNIP expression.
AuthorsQian S,Liu J,Liao W,Wang F
JournalGenes & genomics
PubMed ID37594665
Novel insights into the host immune response of chicken Harderian gland tissue during Newcastle disease virus infection and heat treatment.
AuthorsSaelao P,Wang Y,Gallardo RA,Lamont SJ,Dekkers JM,Kelly T,Zhou H
JournalBMC veterinary research
PubMed ID30208883
BACKGROUND: Newcastle disease virus, in its most pathogenic form, threatens the livelihood of rural poultry farmers where there is a limited infrastructure and service for vaccinations to prevent outbreaks of the virus. Previously reported studies on the host response to Newcastle disease in chickens have not examined the disease under ... More
The amelioration of alcohol-induced liver and intestinal barrier injury by Lactobacillus rhamnosus Gorbach-Goldin (LGG) is dependent on Interleukin 22 (IL-22) expression.
AuthorsGe Y,Sun H,Xu L,Zhang W,Lv J,Chen Y
JournalBioengineered
PubMed ID35603884
Alcoholic liver disease (ALD) is a common clinical liver injury disease. Lactobacillus rhamnosus Gorbach-Goldin (LGG) has been revealed to alleviate alcohol-induced intestinal barrier and liver injury. However, the underlying mechanism of LGG treatment for ALD remains unclear. To clarify this aspect, a chronic plus binge ALD model was constructed using ... More
Particulate matter 10 exposure affects intestinal functionality in both inflamed 2D intestinal epithelial cell and 3D intestinal organoid models.
AuthorsSon YS,Son N,Yu WD,Baek A,Park YJ,Lee MS,Lee SJ,Kim DS,Son MY
JournalFrontiers in immunology
PubMed ID37435069
BACKGROUND: A growing body of evidence suggests that particulate matter (PM10) enters the gastrointestinal (GI) tract directly, causing the GI epithelial cells to function less efficiently, leading to inflammation and an imbalance in the gut microbiome. PM10 may, however, act as an exacerbation factor in patients with inflamed intestinal epithelium, ... More
Real-time PCR Analysis of PAMP-induced Marker Gene Expression in Nicotiana benthamiana.
AuthorsLiu F,Xu Y,Wang Y,Wang Y
JournalBio-protocol
PubMed ID34532510
Perception of pathogen-associated molecular patterns (PAMPs) often triggers various innate immune responses in plants. The transcriptional changes of defense-related genes are often used as a marker to assay PAMP-triggered plant immune response. Here we described a protocol to monitor the relative expression level of marker genes in Nicotiana benthamiana upon ... More
MFN2 Deficiency Impairs Mitochondrial Functions and PPAR Pathway During Spermatogenesis and Meiosis in Mice.
JournalFrontiers in cell and developmental biology
PubMed ID35493072
Mitochondria are highly dynamic organelles and their activity is known to be regulated by changes in morphology via fusion and fission events. However, the role of mitochondrial dynamics on cellular differentiation remains largely unknown. Here, we explored the molecular mechanism of mitochondrial fusion during spermatogenesis by generating an Mfn2 (mitofusin ... More
When Electrospun Fiber Support Matters: In Vitro Ovine Long-Term Folliculogenesis on Poly (Epsilon Caprolactone) (PCL)-Patterned Fibers.
Current assisted reproduction technologies (ART) are insufficient to cover the slice of the population needing to restore fertility, as well as to amplify the reproductive performance of domestic animals or endangered species. The design of dedicated reproductive scaffolds has opened the possibility to better recapitulate the reproductive 3D ovarian environment, ... More
Concordance of Genomic Alterations between Circulating Tumor DNA and Matched Tumor Tissue in Chinese Patients with Breast Cancer.
AuthorsXu B,Shan G,Wu Q,Li W,Wang H,Li H,Yang Y,Long Q,Zhao P
JournalJournal of oncology
PubMed ID32908507
PURPOSE: Circulating tumor DNA (ctDNA) served as a noninvasive method with less side effects using peripheral blood. Given the studies on concordance rate between liquid and solid biopsies in Chinese breast cancer (BC) patients were limited, we sought to examine the concordance rate of different kinds of genomic alterations between ... More
Unique Interplay Between Molecular miR-181b/d Biomarkers and Health Related Quality of Life Score in the Predictive Glioma Models.
AuthorsStakaitis R,Pranckeviciene A,Steponaitis G,Tamasauskas A,Bunevicius A,Vaitkiene P
JournalInternational journal of molecular sciences
PubMed ID33050332
In the last decade, an increasing amount of research has been conducted analyzing microRNA expression changes in glioma tissue and its expressed exosomes, but there is still sparse information on microRNAs or other biomarkers and their association with patientsâ functional/psychological outcomes. In this study, we performed a combinational analysis measuring ... More
Regulation of Tomato Fruit Autophagic Flux and Promotion of Fruit Ripening by the Autophagy-Related Gene SlATG8f.
AuthorsWen C,Luo T,He Z,Li Y,Yan J,Xu W
JournalPlants (Basel, Switzerland)
PubMed ID37765504
Autophagy is a highly conserved self-degradation process that involves the degradation and recycling of cellular components and organelles. Although the involvement of autophagy in metabolic changes during fruit ripening has been preliminarily demonstrated, the variations in autophagic flux and specific functional roles in tomato fruit ripening remain to be elucidated. ... More
Whole Genome DNA Methylation Profiling of D2 Medium Spiny Neurons in Mouse Nucleus Accumbens Using Two Independent Library Preparation Methods.
AuthorsLi Y,Xu H,Chitaman JM,Feng J
JournalGenes
PubMed ID35205351
DNA methylation plays essential roles in various cellular processes. Next-generation sequencing has enabled us to study the functional implication of DNA methylation across the whole genome. However, this approach usually requires a substantial amount of genomic DNA, which limits its application to defined cell types within a discrete brain region. ... More
ClpP/ClpX deficiency impairs mitochondrial functions and mTORC1 signaling during spermatogenesis.
Caseinolytic protease proteolytic subunit (ClpP) and caseinolytic protease X (ClpX) are mitochondrial matrix peptidases that activate mitochondrial unfolded protein response to maintain protein homeostasis in the mitochondria. However, the role of ClpP and ClpX in spermatogenesis remains largely unknown. In this study, we demonstrated the importance of ClpP/ClpX for meiosis ... More
Retinoic acid modulates chromatin to potentiate tumor necrosis factor alpha signaling on the DIF2 promoter.
AuthorsWitcher M, Pettersson F, Dupere-Richer D, Padovani A, Summers-Deluca L, Baldwin AS, Miller WH Jr
JournalNucleic Acids Res
PubMed ID18039708
'Transcriptional activation by nuclear hormone receptors is well characterized, but their cooperation with other signaling pathways to activate transcription remains poorly understood. Tumor necrosis factor alpha (TNFalpha) and all-trans retinoic acid (RA) induce monocytic differentiation of acute promyelocytic leukemia (APL) cells in a synergistic manner. We used the promoter of ... More
Clinically distinct epigenetic subgroups in Silver-Russell syndrome: the degree of H19 hypomethylation associates with phenotype severity and genital and skeletal anomalies.
AuthorsBruce S, Hannula-Jouppi K, Peltonen J, Kere J, Lipsanen-Nyman M
JournalJ Clin Endocrinol Metab
PubMed ID19017756
'CONTEXT: The H19 imprinting control region (ICR), located on chromosome 11p15.5, has been reported hypomethylated in 20-65% of Silver-Russell syndrome (SRS) patients. OBJECTIVE: We investigated the methylation status of 11p15.5 ICRs in SRS patients and children born small for gestational age (SGA) to clarify the relationship between phenotype and ... More
CNTNAP2 and NRXN1 Are Mutated in Autosomal-Recessive Pitt-Hopkins-like Mental Retardation and Determine the Level of a Common Synaptic Protein in Drosophila
AuthorsZweier, C; de Jong, EK; Zweier, M; Orrico, A; Ousager, LB; Collins, AL; Bijlsma, EK; Oortveld, MAW; Ekici, AB; Reis, A; Schenck, A; Rauch, A
Journal
PubMed ID
'Heterozygous copy-number variants and SNPs of CNTNAP2 and NRXN1, two distantly related members of the neurexin superfamily, have been repeatedly associated with a wide spectrum of neuropsychiatric disorders, such as developmental language disorders, autism spectrum disorders, epilepsy, and schizophrenia. We now identified homozygous and compound-heterozygous deletions and mutations via molecular ... More
Blockade of the ubiquitin protease UBP43 destabilizes transcription factor PML/RARalpha and inhibits the growth of acute promyelocytic leukemia.
AuthorsGuo Y, Dolinko AV, Chinyengetere F, Stanton B, Bomberger JM, Demidenko E, Zhou DC, Gallagher R, Ma T, Galimberti F, Liu X, Sekula D, Freemantle S, Dmitrovsky E
JournalCancer Res
PubMed ID20935222
'More effective treatments for acute promyelocytic leukemia (APL) are needed. APL cell treatment with all-trans-retinoic acid (RA) degrades the chimeric, dominant-negative-acting transcription factor promyelocytic leukemia gene (PML)/RARalpha, which is generated in APL by chromosomal translocation. The E1-like ubiquitin-activating enzyme (UBE1L) associates with interferon-stimulated gene ISG15 that binds and represses PML/RARalpha ... More
Inhibition of cyclooxygenase-2 suppresses lymph node metastasis via reduction of lymphangiogenesis.
AuthorsIwata C, Kano MR, Komuro A, Oka M, Kiyono K, Johansson E, Morishita Y, Yashiro M, Hirakawa K, Kaminishi M, Miyazono K
JournalCancer Res
PubMed ID17974958
'Cyclooxygenase-2 (COX-2) inhibitor has been reported to suppress tumor progression. However, it is unclear whether this inhibitor can also prevent lymphatic metastasis. To determine the effects of COX-2 inhibitor on lymphatic metastasis, etodolac, a COX-2 inhibitor, was given p.o. to mice bearing orthotopic xenografts or with carcinomatous peritonitis induced with ... More
Sirtuin 1 is required for antagonist-induced transcriptional repression of androgen-responsive genes by the androgen receptor.
AuthorsDai Y, Ngo D, Forman LW, Qin DC, Jacob J, Faller DV
JournalMol Endocrinol
PubMed ID17505061
'Androgen antagonists or androgen deprivation is a primary therapeutic modality for the treatment of prostate cancer. Invariably, however, the disease becomes progressive and unresponsive to androgen ablation therapy (hormone refractory). The molecular mechanisms by which the androgen antagonists inhibit prostate cancer proliferation are not fully defined. In this report, we ... More
MicroRNA-223 regulates Glut4 expression and cardiomyocyte glucose metabolism.
AuthorsLu H, Buchan RJ, Cook SA
JournalCardiovasc Res
PubMed ID20080987
'AIMS: MicroRNAs (miRNAs) are important for cardiac function and tissue metabolism. The aim of the present study is to investigate the role(s) of miRNAs in the insulin-resistant heart. METHODS AND RESULTS: Left ventricular biopsies were collected from patients with or without type 2 diabetes and from patients with left ... More
Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
AuthorsGillan S, O'Brien R, Hughes AD, Griffin JF
JournalClin Vaccine Immunol
PubMed ID19923568
'Johne''s disease, a chronic enteritis of ruminants, is caused by infection with Mycobacterium avium subsp. paratuberculosis. Three distinct forms have been observed in sheep: paucibacillary disease (PB), multibacillary disease (MB), and asymptomatic infection (AS). In this study, immune parameters for animals naturally infected with M. avium subsp. paratuberculosis and ... More
Dependence of stress resistance on a spore coat heteropolysaccharide in Dictyostelium.
AuthorsWest CM, Nguyen P, van der Wel H, Metcalf T, Sweeney KR, Blader IJ, Erdos GW
JournalEukaryot Cell
PubMed ID18996984
'In Dictyostelium, sporulation occurs synchronously as prespore cells approach the apex of the aerial stalk during culmination. Each prespore cell becomes surrounded by its own coat comprised of a core of crystalline cellulose and a branched heteropolysaccharide sandwiched between heterogeneous cysteine-rich glycoproteins. The function of the heteropolysaccharide, which consists of ... More
AuthorsSaito RA, Watabe T, Horiguchi K, Kohyama T, Saitoh M, Nagase T, Miyazono K
JournalCancer Res
PubMed ID19293183
'Thyroid transcription factor-1 (TTF-1) is expressed in lung cancer, but its functional roles remain unexplored. TTF-1 gene amplification has been discovered in a part of lung adenocarcinomas, and its action as a lineage-specific oncogene is highlighted. Epithelial-to-mesenchymal transition (EMT) is a crucial event for cancer cells to acquire ... More
Prevalence of t(12;21)[ETV6-RUNX1]-positive cells in healthy neonates.
AuthorsLausten-Thomsen U, Madsen HO, Vestergaard TR, Hjalgrim H, Nersting J, Schmiegelow K
JournalBlood
PubMed ID20713965
't(12;21)(p13;q22)[ETV6-RUNX1] is the most common chromosomal translocation in childhood acute lymphoblastic leukemia, and it can often be backtracked to Guthrie cards supporting prenatal initiation and high levels of circulating t(12;21)-positive cells at birth. To explore the prevalence of ETV6-RUNX1-positive cells in healthy neonates, mononuclear cells from 1417 umbilical cord blood ... More
Development and validation of a multiplex real-time PCR assay for simultaneous genotyping and human T-lymphotropic virus type 1, 2, and 3 proviral load determination.
AuthorsMoens B, Lopez G, Adaui V, Gonzalez E, Kerremans L, Clark D, Verdonck K, Gotuzzo E, Vanham G, Cassar O, Gessain A, Vandamme AM, Van Dooren S
JournalJ Clin Microbiol
PubMed ID19741085
'The human T-lymphotropic virus (HTLV) proviral load remains the best surrogate marker for disease progression. Real-time PCR techniques have been developed for detection and quantification of cosmopolitan HTLV type 1a (HTLV-1a) and HTLV-2. Since a growing level of diversity in subtypes and genotypes is observed, we developed a multiplex ... More
von Hippel-Lindau protein regulates transition from the fetal to the adult circulatory system in retina.
AuthorsKurihara T, Kubota Y, Ozawa Y, Takubo K, Noda K, Simon MC, Johnson RS, Suematsu M, Tsubota K, Ishida S, Goda N, Suda T, Okano H
JournalDevelopment
PubMed ID20388654
'In early neonates, the fetal circulatory system undergoes dramatic transition to the adult circulatory system. Normally, embryonic connecting vessels, such as the ductus arteriosus and the foramen ovale, close and regress. In the neonatal retina, hyaloid vessels maintaining blood flow in the embryonic retina regress, and retinal vessels take over ... More
Activation of paternally expressed genes and perinatal death caused by deletion of the Gtl2 gene.
AuthorsZhou Y, Cheunsuchon P, Nakayama Y, Lawlor MW, Zhong Y, Rice KA, Zhang L, Zhang X, Gordon FE, Lidov HG, Bronson RT, Klibanski A
JournalDevelopment
PubMed ID20610486
'The Dlk1-Gtl2 imprinting locus is located on mouse distal chromosome 12 and consists of multiple maternally expressed non-coding RNAs and several paternally expressed protein-coding genes. The imprinting of this locus plays a crucial role in embryonic development and postnatal growth. At least one cis-element, the intergenic differentially methylated region (IG-DMR) ... More
Genome-wide analysis of mRNA targets for Caenorhabditis elegans FBF, a conserved stem cell regulator.
AuthorsKershner AM, Kimble J
JournalProc Natl Acad Sci U S A
PubMed ID20142496
'Stem cells are essential for tissue generation during the development of multicellular creatures, and for tissue homeostasis in adults. The great therapeutic promise of stem cells makes understanding their regulation a high priority. PUF RNA-binding proteins have a conserved role in promoting self-renewal of germline stem cells. Here we use ... More
Profiling microRNA expression in hepatocellular carcinoma reveals microRNA-224 up-regulation and apoptosis inhibitor-5 as a microRNA-224-specific target.
AuthorsWang Y, Lee AT, Ma JZ, Wang J, Ren J, Yang Y, Tantoso E, Li KB, Ooi LL, Tan P, Lee CG
JournalJ Biol Chem
PubMed ID18319255
'Like other cancers, aberrant gene regulation features significantly in hepatocellular carcinoma (HCC). MicroRNAs (miRNAs) were recently found to regulate gene expression at the post-transcriptional/translational levels. The expression profiles of 157 miRNAs were examined in 19 HCC patients, and 19 up-regulated and 3 down-regulated miRNAs were found to be associated with ... More
Analysis of tetracycline resistance tet(W) genes and their flanking sequences in intestinal Bifidobacterium species.
AuthorsAmmor MS, Florez AB, Alvarez-Martin P, Margolles A, Mayo B
JournalJ Antimicrob Chemother
PubMed ID18614524
'OBJECTIVES: The tet(W) gene provides tetracycline resistance to a wide range of anaerobic intestinal and ruminal bacteria, but little is known about the molecular organization of the tet(W) gene. The aim of this study was to gain new insights into the molecular organization of the tet(W) gene in bifidobacteria ... More
Nuclear cathepsin F regulates activation markers in rat hepatic stellate cells.
AuthorsMaubach G, Lim MC, Zhuo L
JournalMol Biol Cell
PubMed ID18667530
'Activation of hepatic stellate cells during liver fibrosis is a major event facilitating an increase in extracellular matrix deposition. The up-regulation of smooth muscle alpha-actin and collagen type I is indicative of the activation process. The involvement of cysteine cathepsins, a class of lysosomal cysteine proteases, has not been studied ... More
Long-term administration of estradiol decreases expression of hepatic lipogenic genes and improves insulin sensitivity in ob/ob mice: a possible mechanism is through direct regulation of signal transducer and activator of transcription 3.
AuthorsGao H, Bryzgalova G, Hedman E, Khan A, Efendic S, Gustafsson JA, Dahlman-Wright K
JournalMol Endocrinol
PubMed ID16627594
'In this study, we used ob/ob mice as a model to investigate the effects of long-term estradiol administration on insulin sensitivity and to explore the mechanisms that underlie the antidiabetic effects of estrogen on mouse liver. Female ob/ob mice were randomly divided into two groups and given estradiol (100 ... More
Differential regulation of dopaminergic gene expression by Er81.
AuthorsCave JW, Akiba Y, Banerjee K, Bhosle S, Berlin R, Baker H
JournalJ Neurosci
PubMed ID20357122
'A recent study proposed that differentiation of dopaminergic neurons requires a conserved "dopamine motif" (DA-motif) that functions as a binding site for ETS DNA binding domain transcription factors. In the mammalian olfactory bulb (OB), the expression of a set of five genes [including tyrosine hydroxylase (Th)] that are necessary for ... More
Interleukin-10 attenuates vascular responses to endothelin-1 via effects on ERK1/2-dependent pathway.
AuthorsGiachini FR, Zemse SM, Carneiro FS, Lima VV, Carneiro ZN, Callera GE, Ergul A, Webb RC, Tostes RC
JournalAm J Physiol Heart Circ Physiol
PubMed ID19074677
'Interleukin-10 (IL-10) is an anti-inflammatory cytokine with protective actions on the vasculature. On the other hand, endothelin (ET)-1 has potent vasoconstrictor, mitogenic, and proinflammatory activities, which have been implicated in the pathophysiology of a number of cardiovascular diseases. We hypothesized that, in a condition where ET-1 expression is upregulated, i.e., ... More
Environmentally relevant concentrations of 17alpha-ethinylestradiol (EE2) interfere with the growth hormone (GH)/insulin-like growth factor (IGF)-I system in developing bony fish.
AuthorsShved N, Berishvili G, Baroiller JF, Segner H, Reinecke M
JournalToxicol Sci
PubMed ID18660547
'The aim of this study was to evaluate whether effects of environmental estrogens on fish growth and reproduction may be mediated via modulating the growth hormone (GH)/insulin-like growth factor I (IGF-I) system. To this end, developing male and female monosex populations of tilapia were exposed to 17alpha-ethinylestradiol (EE2) at ... More
Expression of hepatitis C virus (HCV) structural proteins in trans facilitates encapsidation and transmission of HCV subgenomic RNA.
'A characteristic of many positive-strand RNA viruses is that, whilst replication of the viral genome is dependent on the expression of the majority of non-structural proteins in cis, virus particle formation can occur when most or all of the structural proteins are co-expressed in trans. Making use of a ... More
Suppressor of cytokine signalling-3 is up-regulated by androgen in prostate cancer cell lines and inhibits androgen-mediated proliferation and secretion.
AuthorsNeuwirt H, Puhr M, Cavarretta IT, Mitterberger M, Hobisch A, Culig Z
JournalEndocr Relat Cancer
PubMed ID18045952
'Suppressors of cytokine signalling (SOCS) are induced by interleukins (ILs) and various peptide hormones and may prevent sustained activation of signalling pathways. We have previously shown that SOCS-3 antagonizes regulation of cellular events by cAMP and is expressed in human prostate cancer. To investigate possible effects of androgen on SOCS-3 ... More
Generation of mice transgenic for human CYP2C18 and CYP2C19: characterization of the sexually dimorphic gene and enzyme expression.
AuthorsLofgren S, Baldwin RM, Hiratsuka M, Lindqvist A, Carlberg A, Sim SC, Schulke M, Snait M, Edenro A, Fransson-Steen R, Terelius Y, Ingelman-Sundberg M
JournalDrug Metab Dispos
PubMed ID18276835
'CYP2C19 is an important enzyme for human drug metabolism, and it also participates in the metabolism of endogenous substrates, whereas the CYP2C18 enzyme is not expressed in human liver despite high mRNA expression. Mice transgenic for the human CYP2C18 and CYP2C19 genes were generated. Quantitative mRNA analysis showed CYP2C18 and ... More
Phosphatidylinositol 4-kinase III beta is a target of enviroxime-like compounds for antipoliovirus activity.
AuthorsArita M, Kojima H, Nagano T, Okabe T, Wakita T, Shimizu H
JournalJ Virol
PubMed ID21177810
'Enviroxime is an antienterovirus compound that targets viral protein 3A and/or 3AB and suppresses a step in enterovirus replication by unknown mechanism. To date, four antienterovirus compounds, i.e., GW5074, Flt3 inhibitor II, TTP-8307, and AN-12-H5, are known to have similar mutations in the 3A protein-encoding region causing resistance to enviroxime ... More
Regulation of soluble guanylyl cyclase-alpha1 expression in chronic hypoxia-induced pulmonary hypertension: role of NFATc3 and HuR.
'The nitric oxide/soluble guanylyl cyclase (sGC) signal transduction pathway plays an important role in smooth muscle relaxation and phenotypic regulation. However, the transcriptional regulation of sGC gene expression is largely unknown. It has been shown that sGC expression increases in pulmonary arteries from chronic hypoxia-induced pulmonary hypertensive animals. Since ... More
DNA methylation and histone modifications cause silencing of Wnt antagonist gene in human renal cell carcinoma cell lines
AuthorsKawamoto, K; Hirata, H; Kikuno, N; Tanaka, Y; Nakagawa, M; Dahiya, R
Journal
PubMed ID
'Secreted frizzled-related protein 2 (sFRP2) is a negative modulator of the Wingless-type (Wnt) signaling pathway, and shown to be inactivated in renal cell carcinoma (RCC). However, the molecular mechanism of silencing of sFRP2 is not fully understood. Our study was designed to elucidate the silencing mechanism of sFRP2 in RCC. ... More
Gene expression profiling defines ATP as a key regulator of human dendritic cell functions.
AuthorsBles N, Horckmans M, Lefort A, Libert F, Macours P, El Housni H, Marteau F, Boeynaems JM, Communi D
JournalJ Immunol
PubMed ID17785789
'Extracellular ATP and PGE2 are two cAMP-elevating agents inducing semimaturation of human monocyte-derived dendritic cells (MoDCs). We have extensively compared the gene expression profiles induced by adenosine 5''-O-(3-thiotriphosphate) (ATPgammaS) and PGE2 in human MoDCs using microarray technology. At 6 h of stimulation, ATPgammaS initiated an impressive expression profile compared ... More
Differential expression of microRNA-675, microRNA-139-3p and microRNA-335 in benign and malignant adrenocortical tumours.
'Background For the clinical management of adrenocortical neoplasms it is crucial to correctly distinguish between benign and malignant tumours. Even histomorphologically based scoring systems do not allow precise separation in single lesions, thus novel parameters are desired which offer a more accurate differentiation. The tremendous potential of microRNAs (miRNAs) as ... More
Activation of Macrophages by Polysaccharide-protein Complex from Lycium barbarum L.
AuthorsChen, ZS; Soo, MY; Srinivasan, N; Tan, BKH; Chan, SH
Journal
PubMed ID
'Macrophages play crucial roles in innate immunity. This paper reports that a polysaccharide-protein complex isolated from Lycium barbarum (LBP) is able to activate macrophages. LBP was isolated from Lycium barbarum fruit and separated to five homogenous fractions, designated LBPF1, LBPF2, LBPF3, LBPF4 and LBPF5. It was found that LBP (50 ... More
Mapping accessible chromatin regions using Sono-Seq.
AuthorsAuerbach RK, Euskirchen G, Rozowsky J, Lamarre-Vincent N, Moqtaderi Z, Lefrancois P, Struhl K, Gerstein M, Snyder M
JournalProc Natl Acad Sci U S A
PubMed ID19706456
'Disruptions in local chromatin structure often indicate features of biological interest such as regulatory regions. We find that sonication of cross-linked chromatin, when combined with a size-selection step and massively parallel short-read sequencing, can be used as a method (Sono-Seq) to map locations of high chromatin accessibility in promoter regions. ... More
A gain-of-function mutation of Arabidopsis cryptochrome1 promotes flowering.
AuthorsExner V, Alexandre C, Rosenfeldt G, Alfarano P, Nater M, Caflisch A, Gruissem W, Batschauer A, Hennig L
JournalPlant Physiol
PubMed ID20926618
'Plants use different classes of photoreceptors to collect information about their light environment. Cryptochromes are blue light photoreceptors that control deetiolation, entrain the circadian clock, and are involved in flowering time control. Here, we describe the cry1-L407F allele of Arabidopsis (Arabidopsis thaliana), which encodes a hypersensitive cryptochrome1 (cry1) protein. Plants ... More
Hypoglycemia from IGF2 overexpression associated with activation of fetal promoters and loss of imprinting in a metastatic hemangiopericytoma.
AuthorsLawson EA, Zhang X, Crocker JT, Wang WL, Klibanski A
JournalJ Clin Endocrinol Metab
PubMed ID19383775
'CONTEXT: The mechanism of IGF2 overexpression in non-islet-cell tumor hypoglycemia is not understood. OBJECTIVE: We investigated the imprinting control and promoter usage for IGF2 expression to identify a mechanism for increased IGF-II production in non-islet-cell tumor hypoglycemia. PATIENT AND METHODS: A patient with metastatic hemangiopericytoma was studied. Tissue from the ... More
The role of interleukin-6 in lipopolysaccharide-induced fever by mechanisms independent of prostaglandin E2.
AuthorsNilsberth C, Elander L, Hamzic N, Norell M, Lonn J, Engstrom L, Blomqvist A
JournalEndocrinology
PubMed ID19022895
'Fever has been shown to be elicited by prostaglandin E(2) (PGE(2)) binding to its receptors on thermoregulatory neurons in the anterior hypothalamus. The signals that trigger PGE(2) production are thought to include proinflammatory cytokines, such as IL-6. However, although the presence of IL-6 is critical for fever, IL-6 by itself ... More
Host structural carbohydrate induces vector transmission of a bacterial plant pathogen.
AuthorsKilliny N, Almeida RP
JournalProc Natl Acad Sci U S A
PubMed ID20018775
'Many insect-borne pathogens have complex life histories because they must colonize both hosts and vectors for successful dissemination. In addition, the transition from host to vector environments may require changes in gene expression before the pathogen''s departure from the host. Xylella fastidiosa is a xylem-limited plant-pathogenic bacterium transmitted by leafhopper ... More
Inflammatory interaction between LIGHT and proteinase-activated receptor-2 in endothelial cells: potential role in atherogenesis.
AuthorsSandberg WJ, Halvorsen B, Yndestad A, Smith C, Otterdal K, Brosstad FR, Froland SS, Olofsson PS, Damas JK, Gullestad L, Hansson GK, Oie E, Aukrust P
JournalCirc Res
PubMed ID19023130
'The interaction between inflammatory cytokines and endothelial cells is a critical step in atherogenesis leading to endothelial dysfunction and inflammation. We have previously reported that the tumor necrosis factor superfamily member LIGHT could be involved in atherogenesis through its ability to promote vascular inflammation. In the present study we identified ... More
Elevated expression of osteopontin may be related to adipose tissue macrophage accumulation and liver steatosis in morbid obesity.
AuthorsBertola A, Deveaux V, Bonnafous S, Rousseau D, Anty R, Wakkach A, Dahman M, Tordjman J, Clement K, McQuaid SE, Frayn KN, Huet PM, Gugenheim J, Lotersztajn S, Le Marchand-Brustel Y, Tran A, Gual P
JournalDiabetes
PubMed ID18952835
'OBJECTIVE: Osteopontin (OPN) plays an important role in the development of insulin resistance and liver complications in dietary murine models. We aimed to determine the expression pattern of OPN and its receptor CD44 in obese patients and mice according to insulin resistance and liver steatosis. RESEARCH DESIGN AND METHODS: OPN ... More
Efflux-related resistance to norfloxacin, dyes, and biocides in bloodstream isolates of Staphylococcus aureus.
AuthorsDeMarco CE, Cushing LA, Frempong-Manso E, Seo SM, Jaravaza TA, Kaatz GW
JournalAntimicrob Agents Chemother
PubMed ID17576828
'Efflux is an important resistance mechanism in Staphylococcus aureus, but its frequency in patients with bacteremia is unknown. Nonreplicate bloodstream isolates were collected over an 8-month period, and MICs of four common efflux pump substrates, with and without the broad-spectrum efflux pump inhibitor reserpine, were determined (n = 232). A ... More
Bacillus polyfermenticus ameliorates colonic inflammation by promoting cytoprotective effects in colitic mice.
AuthorsIm E, Choi YJ, Pothoulakis C, Rhee SH
JournalJ Nutr
PubMed ID19675103
'Although human consumption of Bacillus polyfermenticus provides several health benefits, the probiotic effect of this bacterium against colonic inflammation has not yet, to our knowledge, been studied. Therefore, we induced colitis in mice by oral or intrarectal administration of dextran sodium sulfate (DSS) or trinitrobenzenosulfonic acid (TNBS), respectively, and investigated ... More
Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in oral cancer.
AuthorsKozaki K, Imoto I, Mogi S, Omura K, Inazawa J
JournalCancer Res
PubMed ID18381414
'In the last few years, microRNAs (miRNA) have started a revolution in molecular biology and emerged as key players in the carcinogenesis. They have been identified in various tumor types, showing that different sets of miRNAs are usually deregulated in different cancers. To identify the miRNA signature that was specific ... More
Chronic furosemide or hydrochlorothiazide administration increases H+-ATPase B1 subunit abundance in rat kidney.
AuthorsNa KY, Kim GH, Joo KW, Lee JW, Jang HR, Oh YK, Jeon US, Chae SW, Knepper MA, Han JS
JournalAm J Physiol Renal Physiol
PubMed ID17311909
'Furosemide administration stimulates distal acidification. This has been attributed to the increased lumen-negative voltage in the distal nephron, but the aspect of regulatory mechanisms of H(+)-ATPase has not been clear. The purpose of this study is to investigate whether chronic administration of diuretics alters the expression of H(+)-ATPase and whether ... More
Targeting the cyclin E-Cdk-2 complex represses lung cancer growth by triggering anaphase catastrophe.
AuthorsGalimberti F, Thompson SL, Liu X, Li H, Memoli V, Green SR, DiRenzo J, Greninger P, Sharma SV, Settleman J, Compton DA, Dmitrovsky E
JournalClin Cancer Res
PubMed ID20028770
'PURPOSE: Cyclin-dependent kinases (Cdk) and their associated cyclins are targets for lung cancer therapy and chemoprevention given their frequent deregulation in lung carcinogenesis. This study uncovered previously unrecognized consequences of targeting the cyclin E-Cdk-2 complex in lung cancer. EXPERIMENTAL DESIGN: Cyclin E, Cdk-1, and Cdk-2 were individually ... More
Identification of transcriptional regulatory cascades in retinoic acid-induced growth arrest of HepG2 cells.
AuthorsNakanishi M, Tomaru Y, Miura H, Hayashizaki Y, Suzuki M
JournalNucleic Acids Res
PubMed ID18445634
'All-trans retinoic acid (ATRA) is a potent inducer of cell differentiation and growth arrest. Here, we investigated ATRA-induced regulatory cascades associated with growth arrest of the human hepatoma cell line HepG2. ATRA induced >2-fold changes in the expression of 402 genes including 55 linked to cell-cycle regulation, cell growth ... More
A novel correlation between LINE-1 hypomethylation and the malignancy of gastrointestinal stromal tumors.
AuthorsIgarashi S, Suzuki H, Niinuma T, Shimizu H, Nojima M, Iwaki H, Nobuoka T, Nishida T, Miyazaki Y, Takamaru H, Yamamoto E, Yamamoto H, Tokino T, Hasegawa T, Hirata K, Imai K, Toyota M, Shinomura Y
JournalClin Cancer Res
PubMed ID20978145
'PURPOSE: Gastrointestinal stromal tumors (GIST) are the most important mesenchymal tumors of the gastrointestinal tract. The vast majority of GISTs exhibit activating mutations of KIT or PDGFRA, but epigenetic alteration of GISTs is largely unknown. In this study, we aimed to clarify the involvement of DNA methylation in GIST malignancy. ... More
The transcription factor ATF4 promotes skeletal myofiber atrophy during fasting.
AuthorsEbert SM, Monteys AM, Fox DK, Bongers KS, Shields BE, Malmberg SE, Davidson BL, Suneja M, Adams CM
JournalMol Endocrinol
PubMed ID20197309
'Prolonged fasting alters skeletal muscle gene expression in a manner that promotes myofiber atrophy, but the underlying mechanisms are not fully understood. Here, we examined the potential role of activating transcription factor 4 (ATF4), a transcription factor with an evolutionarily ancient role in the cellular response to starvation. In mouse ... More
Molecular cloning and expression of the CRISP family of proteins in the boar.
AuthorsVadnais ML, Foster DN, Roberts KP
JournalBiol Reprod
PubMed ID18716287
'The family of mammalian cysteine-rich secretory proteins (CRISP) have been well characterized in the rat, mouse, and human. Here we report the molecular cloning and expression analysis of CRISP1, CRISP2, and CRISP3 in the boar. A partial sequence published in the National Center for Biotechnology Information (NCBI) database was ... More
Genome-wide identification of estrogen receptor alpha-binding sites in mouse liver.
AuthorsGao H, Falt S, Sandelin A, Gustafsson JA, Dahlman-Wright K
JournalMol Endocrinol
PubMed ID17901129
'We report the genome-wide identification of estrogen receptor alpha (ERalpha)-binding regions in mouse liver using a combination of chromatin immunoprecipitation and tiled microarrays that cover all nonrepetitive sequences in the mouse genome. This analysis identified 5568 ERalpha-binding regions. In agreement with what has previously been reported for human cell ... More
Blocking the Metabolism of Starch Breakdown Products in Arabidopsis Leaves Triggers Chloroplast Degradation
'In most plants, a large fraction of photo-assimilated carbon is stored in the chloroplasts during the day as starch and remobilized during the subsequent night to support metabolism. Mutations blocking either starch synthesis or starch breakdown in Arabidopsis thaliana reduce plant growth. Maltose is the major product of starch breakdown ... More
A novel membrane fusion-mediated plant immunity against bacterial pathogens
AuthorsHatsugai, N; Iwasaki, S; Tamura, K; Kondo, M; Fuji, K; Ogasawara, K; Nishimura, M; Hara-Nishimura, I
Journal
PubMed ID
'Plants have developed their own defense strategies because they have no immune cells. A common plant defense strategy involves programmed cell death (PCD) at the infection site, but how the PCD-associated cell-autonomous immunity is executed in plants is not fully understood. Here we provide a novel mechanism underlying cell-autonomous immunity, ... More
Thrombospondin-1 peptide ABT-510 combined with valproic acid is an effective antiangiogenesis strategy in neuroblastoma.
AuthorsYang Q, Tian Y, Liu S, Zeine R, Chlenski A, Salwen HR, Henkin J, Cohn SL
JournalCancer Res
PubMed ID17308113
'In the pediatric cancer neuroblastoma, clinically aggressive disease is associated with increased levels of angiogenesis stimulators and high vascular index. We and others have hypothesized that blocking angiogenesis may be effective treatment for this pediatric malignancy. However, little is known about the efficacy of antiangiogenic agents in pediatric malignancies. Recently, ... More
IRAK-4 kinase activity is required for interleukin-1 (IL-1) receptor- and toll-like receptor 7-mediated signaling and gene expression.
AuthorsKoziczak-Holbro M, Joyce C, Gluck A, Kinzel B, Muller M, Tschopp C, Mathison JC, Davis CN, Gram H
JournalJ Biol Chem
PubMed ID17337443
'IRAK-4 is an essential component of the signal transduction complex downstream of the IL-1- and Toll-like receptors. Although regarded as the first kinase in the signaling cascade, the role of IRAK-4 kinase activity versus its scaffold function is still controversial. To investigate the role of IRAK-4 kinase function in vivo, ... More
Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres.
AuthorsMay A, Kirchner R, Muller H, Hartmann P, El Hajj N, Tresch A, Zechner U, Mann W, Haaf T
JournalBiol Reprod
PubMed ID18784354
'We have developed a microfluidic chip-based qualitative assay for sensitive (10 RNA copies) detection of multiple transcripts in single cells. We determined the expression patterns of 17 developmentally important genes and isoforms in individual mouse preimplantation embryos from superovulated matings and blastomeres. The ubiquitously expressed histone variant H3f3a and ... More
Microarray analysis of developing flax hypocotyls identifies novel transcripts correlated with specific stages of phloem fibre differentiation.
AuthorsRoach MJ, Deyholos MK
JournalAnn Bot
PubMed ID18593690
'BACKGROUND AND AIMS: Hypocotyls are a commonly used model to study primary growth in plants, since post-germinative hypocotyls increase in size by cell elongation rather than cell division. Flax hypocotyls produce phloem fibres in bundles one to two cell layers thick, parallel to the protoxylem poles of the stele. ... More
Requirement of c-Jun NH2-Terminal Kinase for Ras-Initiated Tumor Formation.
AuthorsCellurale C, Sabio G, Kennedy NJ, Das M, Barlow M, Sandy P, Jacks T, Davis RJ
JournalMol Cell Biol
PubMed ID21282468
'The c-Jun NH(2)-terminal kinase (JNK) signal transduction pathway causes increased gene expression mediated, in part, by members of the activating transcription factor protein (AP1) group. JNK is therefore implicated in the regulation of cell growth and cancer. To test the role of JNK in Ras-induced tumor formation, we examined the ... More
Antiretroviral pre-exposure prophylaxis prevents vaginal transmission of HIV-1 in humanized BLT mice.
AuthorsDenton PW, Estes JD, Sun Z, Othieno FA, Wei BL, Wege AK, Powell DA, Payne D, Haase AT, Garcia JV
JournalPlos Med
PubMed ID18198941
'BACKGROUND: Worldwide, vaginal transmission now accounts for more than half of newly acquired HIV-1 infections. Despite the urgency to develop and implement novel approaches capable of preventing HIV transmission, this process has been hindered by the lack of adequate small animal models for preclinical efficacy and safety testing. Given the ... More
Target-dependent B7-H1 regulation contributes to clearance of central nervous system infection and dampens morbidity.
AuthorsPhares TW, Ramakrishna C, Parra GI, Epstein A, Chen L, Atkinson R, Stohlman SA, Bergmann CC
JournalJ Immunol
PubMed ID19380790
'The neurotropic coronavirus JHM strain of mouse hepatitis virus persists in oligodendroglia despite the presence of virus-specific CD8 T cells. Expression of programmed death 1 (PD-1) and B7-H1 were studied during acute and persistent infection to examine whether this negative regulatory mechanism contributes to CNS viral persistence. The majority of ... More
DOUBLETIME plays a noncatalytic role to mediate CLOCK phosphorylation and repress CLOCK-dependent transcription within the Drosophila circadian clock.
AuthorsYu W, Zheng H, Price JL, Hardin PE
JournalMol Cell Biol
PubMed ID19139270
'Circadian clocks keep time via gene expression feedback loops that are controlled by time-of-day-specific changes in the synthesis, activity, and degradation of transcription factors. Within the Drosophila melanogaster circadian clock, DOUBLETIME (DBT) kinase is necessary for the phosphorylation of PERIOD (PER), a transcriptional repressor, and CLOCK (CLK), a transcriptional activator, ... More
Growth factors of lower vertebrates: characterization of goldfish (Carassius auratus L.) macrophage colony-stimulating factor-1.
AuthorsHanington PC, Wang T, Secombes CJ, Belosevic M
JournalJ Biol Chem
PubMed ID17827160
'Colony-stimulating factor-1 (CSF-1) regulates mononuclear cell proliferation, differentiation, and survival. The functions of CSF-1 are well documented in mammals; however, little is known about CSF-1 biology in lower vertebrates. This is the first report on the identification and functional characterization of a fish CSF-1 molecule expressed highly in the ... More
Structure of the Plasmodium falciparum circumsporozoite protein, a leading malaria vaccine candidate.
AuthorsPlassmeyer ML, Reiter K, Shimp RL Jr, Kotova S, Smith PD, Hurt DE, House B, Zou X, Zhang Y, Hickman M, Uchime O, Herrera R, Nguyen V, Glen J, Lebowitz J, Jin AJ, Miller LH, MacDonald NJ, Wu Y, Narum DL
JournalJ Biol Chem
PubMed ID19633296
'The Plasmodium falciparum circumsporozoite protein (CSP) is critical for sporozoite function and invasion of hepatocytes. Given its critical nature, a phase III human CSP malaria vaccine trial is ongoing. The CSP is composed of three regions as follows: an N terminus that binds heparin sulfate proteoglycans, a four amino acid ... More
Overexpression of endoplasmic reticulum protein 29 regulates mesenchymal-epithelial transition and suppresses xenograft tumor growth of invasive breast cancer cells
'Endoplasmic reticulum protein 29 (ERp29) is a novel endoplasmic reticulum ( ER) secretion factor that facilitates the transport of secretory proteins in the early secretory pathway. Recently, it was found to be overexpressed in several cancers; however, little is known regarding its function in breast cancer progression. In this study, ... More