TaqMan™ SNP Genotyping Assay, human
Applied Biosystems™
TaqMan™ SNP Genotyping Assay, humanGreen features
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNARead more
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Catalog NumberQuantity
4351379S (300 reactions), made to order
4351374L (2400 reactions), made to order
4351376M (1000 reactions), made to order
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Catalog number 4351379
Price (USD)/ S (300 reactions), Made to Order
344.65
Online exclusive
374.00 
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Quantity:
S (300 reactions), made to order
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.

Our predesigned TaqMan SNP Genotyping Human Assays are a genome-wide collection of millions of human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNPs.

Benefits:
Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
Relevant—extensive collection of predesigned human assays offers direct access to content that is relevant to your research
Tested—all human SNP genotyping assays are functionally tested to ensure allelic discrimination

Approximate ship time
4–6 days in North America and 6–10 days in Europe

TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).

All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix (sold separately): TaqMan Genotyping Master Mix

*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
3'Primer ModificationNone
5'Primer ModificationNone
DescriptionVIC™ - MGB probe detects Allele 1, FAM - MGB probe detects Allele 2
FormFrozen
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler*

* If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR
system in order to detect and record fluorescent signals.
Green FeaturesSustainable packaging
Internal Probe ModificationVIC™ (5'), FAM (5'), MGB (Minor Groove Binder) (3')
Label or DyeFAM, VIC
Product LineTaqMan™
Purification MethodSolid Phase Extraction (SPE)
Purity or Quality GradeRNase-Free, DNase-Free
QuantityS (300 reactions), made to order
Shipping ConditionRoom Temperature
SpeciesHuman
TargetOver 4.5 million genome-wide human assays are available
For Use With (Application)Genotyping
Concentration40X
No. of Reactions300 reactions
Sample TypeHuman
Unit SizeS (300 reactions), Made to Order
Contents & Storage
1 tube containing a 40X (S and M sizes) or 80X (L size) mix of pre-formulated assay (2 probes and 2 primers).

Store at -15 to -25°C.