High-Capacity cDNA Reverse Transcription Kit, 200 reactions - FAQs

Can one use the reagents from High Capacity RT kit to make cDNA for TaqMan MicroRNA Assay?

Yes. You can use the reagents from the High Capacity cDNA Kit PN 4368814 (without RNAse Inhibitor) or 4374966 (with RNAse Inhibitor). Make sure to use the reverse transcription primer provided from the TaqMan miRNA assay for the reverse transcription.

Which kit should I use for the reverse transcription step for TaqMan Pri-miRNA assays (Cat. No. 4427012)?

We recommend the following kits:

• High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4374966).
• High-Capacity RNA-to-cDNA Kit (Cat. No. 4387406).

Is the 25X 100 mM dNTP Mix included in the High Capacity cDNA Reverse Transcription Kit (Cat. No. 4368813, 4368814) 100 mM each or 100 mM combined?

It contains a blend of 100 mM solution of dNTP (25 mM each of dATP, dCTP, dGTP and dTTP).