Preclinical pharmacokinetic and pharmacodynamic evaluation of metronomic and conventional temozolomide dosing regimens.
AuthorsZhou Q, Guo P, Wang X, Nuthalapati S, Gallo JM
JournalJ Pharmacol Exp Ther
PubMed ID17259446
Metronomic dosed (MD) chemotherapy as opposed to conventional dosed (CD) chemotherapy is considered an alternate strategy to target angiogenesis and limit host toxicity. Although this approach is promising, there has not been any attempt to define optimal metronomic dosing regimens by integrating pharmacokinetic (PK) with pharmacodynamic (PD) measurements. The aim ... More
Aim2 Deficiency Stimulates the Expression of IFN-Inducible Ifi202, a Lupus Susceptibility Murine Gene within the Nba2 Autoimmune Susceptibility Locus.
AuthorsPanchanathan R, Duan X, Shen H, Rathinam VA, Erickson LD, Fitzgerald KA, Choubey D
JournalJ Immunol
PubMed ID21057088
Murine Aim2 and p202 proteins (encoded by the Aim2 and Ifi202 genes) are members of the IFN-inducible p200 protein family. Both proteins can sense dsDNA in the cytoplasm. However, upon sensing dsDNA, only the Aim2 protein through its pyrin domain can form an inflammasome to activate caspase-1 and induce cell ... More
ADP-ribosylation factor-like GTPase ARFRP1 is required for trans-Golgi to plasma membrane trafficking of E-cadherin.
AuthorsZahn C, Jaschke A, Weiske J, Hommel A, Hesse D, Augustin R, Lu L, Hong W, Florian S, Scheepers A, Joost HG, Huber O, Schurmann A
JournalJ Biol Chem
PubMed ID18662990
ADP-ribosylation factor-related protein 1 (ARFRP1) plays a specific role in Golgi function controlling recruitment of GRIP domain proteins and ARL1 to the trans-Golgi. Deletion of the mouse Arfrp1 gene causes embryonic lethality during early gastrulation, because epiblast cells detach from the ectodermal cell layer and do not differentiate to mesodermal ... More
Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development.
AuthorsOhnishi Y, Totoki Y, Toyoda A, Watanabe T, Yamamoto Y, Tokunaga K, Sakaki Y, Sasaki H, Hohjoh H
JournalNucleic Acids Res
PubMed ID20385573
Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early mammalian development remains poorly understood. We investigated the expression profiles of small RNAs in mouse metaphase II ... More
Successful immunotherapy with IL-2/anti-CD40 induces the chemokine-mediated mitigation of an immunosuppressive tumor microenvironment.
Treatment of mice bearing orthotopic, metastatic tumors with anti-CD40 antibody resulted in only partial, transient anti-tumor effects whereas combined treatment with IL-2/anti-CD40, induced tumor regression. The mechanisms for these divergent anti-tumor responses were examined by profiling tumor-infiltrating leukocyte subsets and chemokine expression within the tumor microenvironment after immunotherapy. IL-2/anti-CD40, but ... More
Acute Lung Injury but not Sepsis is Associated with Increased Colony Formation by Peripheral Blood Mononuclear Cells.
AuthorsBurnham EL, Mealer M, Gaydos J, Majka S, Moss M
JournalAm J Respir Cell Mol Biol
PubMed ID19843706
""Acute lung injury (ALI) and severe sepsis are common critical illnesses associated with mobilization of bone marrow-derived cells into circulation. By identifying and determining these cellsa functional characteristics, unique prognostic biomarkers can be developed to help investigators understand mechanisms underlying the pathophysiology of these disorders. We previously demonstrated increased colony ... More
Human UPF1 participates in small RNA-induced mRNA downregulation.
AuthorsJin H, Suh MR, Han J, Yeom KH, Lee Y, Heo I, Ha M, Hyun S, Kim VN
JournalMol Cell Biol
PubMed ID19704008
MicroRNAs (miRNAs) are endogenous antisense regulators that trigger endonucleolytic mRNA cleavage, translational repression, and/or mRNA decay. miRNA-mediated gene regulation is important for numerous biological pathways, yet the underlying mechanisms are still under rigorous investigation. Here we identify human UPF1 (hUPF1) as a protein that contributes to RNA silencing. When ... More
AuthorsYu H, Riederer B, Stieger N, Boron WF, Shull GE, Manns MP, Seidler UE, Bachmann O
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID19779011
A Na(+)/HCO(3)(-) cotransporter (NBC) is located in the basolateral membrane of the gastrointestinal epithelium, where it imports HCO(3)(-) during stimulated anion secretion. Having previously demonstrated secretagogue activation of NBC in murine colonic crypts, we now asked whether vesicle traffic and exocytosis are involved in this process. Electrogenic NBCe1-B was expressed ... More
Could the differences in O(3) sensitivity between two poplar clones be related to a difference in antioxidant defense and secondary metabolic response to O(3) influx?
AuthorsDi Baccio D, Castagna A, Paoletti E, Sebastiani L, Ranieri A
JournalTree Physiol
PubMed ID19193559
Increasing global background concentrations of tropospheric ozone (O(3)) are expected to affect both crops and forest ecosystems negatively. The phytotoxic effects of O(3) are mainly associated with the O(3)-induced production of reactive oxygen species (ROS) in excess of the ability of the plant to maintain ROS below the tolerance threshold. ... More
Mouse adenovirus type 1-induced breakdown of the blood-brain barrier.
AuthorsGralinski LE, Ashley SL, Dixon SD, Spindler KR
JournalJ Virol
PubMed ID19570856
Infection with mouse adenovirus type 1 (MAV-1) results in fatal acute encephalomyelitis in susceptible mouse strains via infection of brain endothelial cells. Wild-type (wt) MAV-1 causes less brain inflammation than an early region 3 (E3) null virus in C57BL/6 mice. A mouse brain microvascular endothelial cell line infected with wt ... More
Expression of claudin7 is tightly associated with epithelial structures in synovial sarcomas and regulated by an Ets family transcription factor, ELF3.
AuthorsKohno Y, Okamoto T, Ishibe T, Nagayama S, Shima Y, Nishijo K, Shibata KR, Fukiage K, Otsuka S, Uejima D, Araki N, Naka N, Nakashima Y, Aoyama T, Nakayama T, Nakamura T, Toguchida J
JournalJ Biol Chem
PubMed ID17060315
Synovial sarcoma, a soft tissue sarcoma that develops in adults, is pathologically subclassified into monophasic spindle synovial sarcoma and biphasic synovial sarcoma with epithelial components. The molecular mechanism building the epithelial components in biphasic synovial sarcoma is totally unknown. Here we investigated claudins, critical molecules in the tight junction, in ... More
Bone marrow-derived cells require a functional glucose 6-phosphate transporter for normal myeloid functions.
Glycogen storage disease type Ib (GSD-Ib) is caused by a deficiency in the ubiquitously expressed glucose 6-phosphate transporter (Glc-6-PT). Glc-6-PT activity has been shown to be critical in the liver and kidney where a deficiency disrupts glucose homeostasis. GSD-Ib patients also have defects in the neutrophil respiratory burst, chemotaxis, and ... More
Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells.
AuthorsImamura M, Miura K, Iwabuchi K, Ichisaka T, Nakagawa M, Lee J, Kanatsu-Shinohara M, Shinohara T, Yamanaka S
JournalBmc Dev Biol
PubMed ID16859545
BACKGROUND: We previously identified a set of genes called ECATs (ES cell-associated transcripts) that are expressed at high levels in mouse ES cells. Here, we examine the expression and DNA methylation of ECATs in somatic cells and germ cells. RESULTS: In all ECATs examined, the promoter region had low methylation ... More
Dual effect of APOBEC3G on Hepatitis B virus.
AuthorsNoguchi C, Hiraga N, Mori N, Tsuge M, Imamura M, Takahashi S, Fujimoto Y, Ochi H, Abe H, Maekawa T, Yatsuji H, Shirakawa K, Takaori-Kondo A, Chayama K
JournalJ Gen Virol
PubMed ID17251560
G to A hypermutation of Hepatitis B virus (HBV) and retroviruses appears as a result of deamination activities of host APOBEC proteins and is thought to play a role in innate antiviral immunity. Alpha and gamma interferons (IFN-alpha and -gamma) have been reported to upregulate the transcription of APOBEC3G, which ... More
Loss of caveolin-1 in bronchiolization in lung fibrosis.
AuthorsOdajima N, Betsuyaku T, Nasuhara Y, Nishimura M
JournalJ Histochem Cytochem
PubMed ID17478448
Bronchiolization is a key process in fibrosing lung in which the proliferative status of bronchiolar epithelium changes, leading to abnormal epithelial morphology. Within the context that caveolin-1 acts to suppress epithelial proliferation, we postulated that stimulating epithelial injury would lead to caveolin-1 downregulation and encourage proliferation. The present study evaluates ... More
Inhibition of ICMT induces endothelial cell apoptosis through GRP94.
AuthorsLu Q, Harrington EO, Newton J, Jankowich M, Rounds S
JournalAm J Respir Cell Mol Biol
PubMed ID17347446
Isoprenylcysteine-O-carboxyl methyltransferase (ICMT) catalyzes methylation of proteins containing a C-terminal CAAX motif. We have previously shown that chemical inhibition of ICMT caused endothelial cell apoptosis, an effect correlated with decreased Ras and RhoA carboxyl methylation and GTPase activities. In the current study, proteomic analysis of pulmonary artery endothelial cells (PAEC) ... More
CABYR is a novel cancer-testis antigen in lung cancer.
AuthorsLuo C, Xiao X, Liu D, Chen S, Li M, Xu A, Liu J, Gao S, Wu S, He D
JournalClin Cancer Res
PubMed ID17317841
PURPOSE: Cancer-testis (CT) antigens are often expressed in a proportion of tumors of various types. Their restricted normal tissue expression and immunogenicity make them potential targets for immunotherapy. CABYR is a calcium-binding tyrosine phosphorylation-regulated fibrous sheath protein initially reported to be testis specific and subsequently shown to be present in ... More
High frequency of BMPR2 exonic deletions/duplications in familial pulmonary arterial hypertension.
AuthorsCogan JD, Pauciulo MW, Batchman AP, Prince MA, Robbins IM, Hedges LK, Stanton KC, Wheeler LA, Phillips JA 3rd, Loyd JE, Nichols WC
JournalAm J Respir Crit Care Med
PubMed ID16728714
RATIONALE: Previous studies have shown that approximately 55% of patients with familial pulmonary arterial hypertension (FPAH) have BMPR2 coding sequence mutations. However, direct sequencing does not detect other types of heterozygous mutations, such as exonic deletions/duplications. OBJECTIVE: To estimate the frequency of BMPR2 exonic deletions/duplications in FPAH. METHODS: BMPR2 mRNA ... More
Inhibition of interleukin-22 attenuates bacterial load and organ failure during acute polymicrobial sepsis.
Interleukin-22 (IL-22) is a recently discovered proinflammatory cytokine, structurally related to IL-10. Since IL-22 is induced by lipopolysaccharide in vivo, we studied the role of IL-22 in a model of polymicrobial peritonitis. Quantitative real-time reverse transcription-PCR analysis showed marked induction of IL-22 and IL-22 receptor in spleen and kidney during ... More
Norepinephrine up-regulates the expression of vascular endothelial growth factor, matrix metalloproteinase (MMP)-2, and MMP-9 in nasopharyngeal carcinoma tumor cells.
AuthorsYang EV, Sood AK, Chen M, Li Y, Eubank TD, Marsh CB, Jewell S, Flavahan NA, Morrison C, Yeh PE, Lemeshow S, Glaser R
JournalCancer Res
PubMed ID17079456
Recent studies using ovarian cancer cells have shown that the catecholamine hormones norepinephrine (norepi) and epinephrine (epi) may influence cancer progression by modulating the expression of matrix metalloproteinases (MMP) and vascular endothelial growth factor (VEGF). The purpose of this study is to determine if the stress hormone norepi can influence ... More
Changes in PDE4D isoforms in the hippocampus of a patient with advanced Alzheimer disease.
Gustatory expression pattern of the human TAS2R bitter receptor gene family reveals a heterogenous population of bitter responsive taste receptor cells.
AuthorsBehrens M, Foerster S, Staehler F, Raguse JD, Meyerhof W
JournalJ Neurosci
PubMed ID18003842
Human bitter taste is mediated by approximately 25 members of the human TAS2 receptor (hTAS2R) gene family. The hTAS2R genes are expressed in taste buds of gustatory papillae on the tongue surface. Because many naturally occurring bitter compounds are toxic, bitter taste receptors are believed to serve as warning sensors ... More
Differential gene expression in functional classes of interstitial cells of Cajal in murine small intestine.
AuthorsChen H, Ordog T, Chen J, Young DL, Bardsley MR, Redelman D, Ward SM, Sanders KM
JournalPhysiol Genomics
PubMed ID17895395
Interstitial cells of Cajal (ICC) have important functions in regulation of motor activity in the gastrointestinal tract. In murine small intestine, ICC are gathered in the regions of the myenteric plexus (ICC-MY) and the deep muscular plexus (ICC-DMP). These two classes of ICC have different physiological functions. ICC-MY are pacemaker ... More
Cell- and gene-specific regulation of primary target genes by the androgen receptor.
AuthorsBolton EC, So AY, Chaivorapol C, Haqq CM, Li H, Yamamoto KR
JournalGenes Dev
PubMed ID17699749
The androgen receptor (AR) mediates the physiologic and pathophysiologic effects of androgens including sexual differentiation, prostate development, and cancer progression by binding to genomic androgen response elements (AREs), which influence transcription of AR target genes. The composition and context of AREs differ between genes, thus enabling AR to confer ... More
Different effects of SLCO1B1 polymorphism on the pharmacokinetics and pharmacodynamics of repaglinide and nateglinide.
AuthorsKalliokoski A, Neuvonen M, Neuvonen PJ, Niemi M
JournalJ Clin Pharmacol
PubMed ID18187595
Thirty-two healthy volunteers with different SLCO1B1 genotypes ingested a 0.5-mg dose of repaglinide and 60-mg dose of nateglinide with a washout period of 1 week. Participants with SLCO1B1 c.521CC genotype (n = 4) had a 59% (P = 0.001) or 72% (P < 0.001) greater mean area under the ... More
All-trans retinoic acid modulates radiation-induced proliferation of lung fibroblasts via IL-6/IL-6R system.
AuthorsTabata C, Kubo H, Tabata R, Wada M, Sakuma K, Ichikawa M, Fujita S, Mio T, Mishima M
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID16257998
Although high-dose thoracic radiotherapy is an effective strategy for some malignancies including lung cancers and malignant lymphomas, it often causes complications of radiation fibrosis. To study the mechanism initiating tissue fibrosis, we investigated irradiation-induced cytokine production from human lung fibroblastic cells and found that IL-6 production was stimulated by irradiation. ... More
Use of multiplex PCR and real-time PCR to detect human herpes virus genome in ocular fluids of patients with uveitis
AuthorsSugita, S; Shimizu, N; Watanabe, K; Mizukami, M; Morio, T; Sugamoto, Y; Mochizuki, M
JournalBRITISH JOURNAL OF OPHTHALMOLOGY
PubMed ID
Aim: To measure the genomic DNA of human herpes viruses (HHV) in the ocular fluids and to analyse the clinical relevance of HHV in uveitis. Methods: After informed consent was obtained, a total of 111 ocular fluid samples ( 68 aqueous humour and 43 vitreous fluid samples) were collected ... More
In vivo exposure to high or low cortisol has biphasic effects on inflammatory response pathways of human monocytes.
'BACKGROUND: Recent studies demonstrate that glucocorticoids (GCs) have both supportive (stimulatory) and suppressive effects on immune responses, depending upon the GC concentration. Since some GC effects on inflammation are stimulatory, we hypothesized that acute in vivo GC depletion would decrease inflammatory responses of human monocytes. METHODS: Monocytes were isolated from ... More
Telomere Length in Lymphocytes of Older South Australian Men May Be Inversely Associated with Plasma Homocysteine
'Deficiencies in folate (FOL) and vitamin B12 (B12) result in increased chromosomal aberrations, a validated bio-marker of cancer risk. Telomeres, the regions of DNA that cap the ends of each chromosome, are critical for maintaining chromosomal stability but the impact of micronutrients on telomere structure and function remains unclear. We ... More
EspA, an orphan hybrid histidine protein kinase, regulates the timing of expression of key developmental proteins of Myxococcus xanthus
AuthorsHiggs, PI; Jagadeesan, S; Mann, P; Zusman, DR
Journal
PubMed ID
'Myxococcus xanthus undergoes a complex starvation-induced developmental program that results in cells forming multicellular fruiting bodies by aggregating into mounds and then differentiating into spores. This developmental program requires at least 72 h and is mediated by a temporal cascade of gene regulators in response to intra- and extracellular signals. ... More
Integration of Distinct Intracellular Signaling Pathways at Distal Regulatory Elements Directs T-bet Expression in Human CD4(+) T Cells
AuthorsPlacek, K; Gasparian, S; Coffre, M; Maiella, S; Sechet, E; Bianchi, E; Rogge, L
Journal
PubMed ID
'T-bet is a key regulator controlling Th1 cell development. This factor is not expressed in naive CD4(+) T cells, and the mechanisms controlling expression of T-bet are incompletely understood. In this study, we defined regulatory elements at the human T-bet locus and determined how signals originating at the TCR and ... More
Diabetic conditions promote binding of monocytes to vascular smooth muscle cells and their subsequent differentiation.
AuthorsMeng L, Park J, Cai Q, Lanting L, Reddy MA, Natarajan R
JournalAm J Physiol Heart Circ Physiol
PubMed ID20008269
'Diabetes is associated with significantly accelerated rates of atherosclerosis, key features of which include the presence of excessive macrophage-derived foam cells in the subendothelial space. We examined the hypothesis that enhanced monocyte-vascular smooth muscle cell (VSMC) interactions leading to subendothelial monocyte retention and differentiation to macrophages under diabetic conditions may ... More
Sequence of Atlantic cod (Gadus morhua) GLUT4, GLUT2 and GPDH: Developmental stage expression, tissue expression and relationship to starvation-induced changes in blood glucose.
AuthorsHall JR, Short CE, Driedzic WR
JournalJ Exp Biol
PubMed ID17079719
'cDNAs of putative glucose transporters, GLUT4 and GLUT2, were cloned from Atlantic cod (Gadus morhua). The GLUT4 cDNA encodes a 503 amino acid and the GLUT2 cDNA a 506 amino acid protein. Phylogenetic analysis, amino acid sequence alignment, and tissue distribution support categorizing them as homologues of mammalian GLUT4 and ... More
Arabidopsis NAC Transcription Factor, ANAC078, Regulates Flavonoid Biosynthesis under High-light
'We have isolated a combination of high-light and heat-shock (HLHS) stress-inducible genes, including a NAC transcription factor designated ANAC078. Here we explored the physiological function of ANAC078 under HL stress. Yeast transcription activity assays showed that ANAC078 functions as a transcriptional activator. A fusion protein composed of green fluorescent protein ... More
The expression profile of micro-RNA in endometrium and endometriosis and the influence of ovarian steroids on their expression.
AuthorsPan Q, Luo X, Toloubeydokhti T, Chegini N
JournalMol Hum Reprod
PubMed ID17766684
'MicroRNAs (miRNAs), through mRNA degradation or repression, act as key regulator of gene expression. Our aim was to identify specific miRNAs that are expressed in endometrium of women with and without endometriosis. We profiled the expression of 287 miRNAs in paired eutopic and ectopic endometrium and isolated endometrial ... More
Expression of CD1d and ligand-induced cytokine production are tissue specific in mucosal epithelia of the human lower reproductive tract.
AuthorsKawana K, Matsumoto J, Miura S, Shen L, Kawana Y, Nagamatsu T, Yasugi T, Fujii T, Yang H, Quayle AJ, Taketani Y, Schust DJ
JournalInfect Immun
PubMed ID18458073
'Mucosal epithelia of the human lower reproductive tract (vagina, cervix, and penile urethra) are exposed to sexually transmitted microbes, including Chlamydia trachomatis. The in vivo susceptibility of each tissue type to infection with C. trachomatis is quite distinct. CD1d is expressed on the surface of antigen-presenting cells, including mucosal ... More
Costimulation of dendritic epidermal gammadelta T cells by a new NKG2D ligand expressed specifically in the skin.
AuthorsWhang MI, Guerra N, Raulet DH
JournalJ Immunol
PubMed ID19342629
'Dendritic epidermal T cells (DETCs) are a highly specialized population of gammadelta T cells that resides in the murine skin and participates in wound healing and tumor surveillance. Despite the expression of other stimulatory receptors on these cells, mechanisms involving activation have focused primarily on the invariant Vgamma3-Vdelta1 TCR ... More
An Adipose Tissue-Independent Insulin-Sensitizing Action of Telmisartan: a Study in Lipodystrophic Mice
AuthorsRong, X; Li, Y; Ebihara, K; Zhao, M; Aini, W; Kusakabe, T; Hirata, M; Miyamoto, L; Murray, M; Nakao, K
Journal
PubMed ID
'Adipose tissue plays an important role in energy balance and metabolism and is the major target for insulin-sensitizing peroxisome proliferator-activated receptor (PPAR) γ agonists. The angiotensin II type 1 receptor blocker telmisartan, a partial agonist of PPAR-γ, has been demonstrated to improve insulin sensitivity. However, there is uncertainty about the ... More
The role of vacuolar processing enzyme (VPE) from Nicotiana benthamiana in the elicitor-triggered hypersensitive response and stomatal closure.
AuthorsZhang H, Dong S, Wang M, Wang W, Song W, Dou X, Zheng X, Zhang Z
JournalJ Exp Bot
PubMed ID20603283
'Elicitors/pathogen-associated molecular patterns (PAMPs) trigger the plant immune system, leading to rapid programmed cell death (hypersensitive response, HR) and stomatal closure. Previous reports have shown that the vacuolar processing enzyme (VPE), a cysteine proteinase responsible for the maturation of vacuolar proteins, has caspase-1-like activity and mediates TMV- and mycotoxin-induced ... More
Expression of human papillomavirus type 16 E7 oncoprotein alters keratinocytes expression profile in response to tumor necrosis factor-alpha.
AuthorsBoccardo E, Manzini Baldi CV, Carvalho AF, Rabachini T, Torres C, Barreta LA, Brentani H, Villa LL
JournalCarcinogenesis
PubMed ID20042637
'Acute expression of E7 oncogene from human papillomavirus (HPV) 16 or HPV18 is sufficient to overcome tumor necrosis factor (TNF)-alpha cytostatic effect on primary human keratinocytes. In the present study, we investigated the molecular basis of E7-induced TNF resistance through a comparative analysis of the effect of this cytokine ... More
A ubiquitin-selective AAA-ATPase mediates transcriptional switching by remodelling a repressor-promoter DNA complex
AuthorsWilcox, AJ; Laney, JD
Journal
PubMed ID
'Switches between different phenotypes and their underlying states of gene transcription occur as cells respond to intrinsic developmental cues or adapt to changing environmental conditions. Post-translational modification of the master regulatory transcription factors that define the initial phenotype is a common strategy to direct such transitions. Emerging evidence indicates that ... More
Ablation of CBP in forebrain principal neurons causes modest memory and transcriptional defects and a dramatic reduction of histone acetylation but does not affect cell viability.
AuthorsValor LM, Pulopulos MM, Jimenez-Minchan M, Olivares R, Lutz B, Barco A
JournalJ Neurosci
PubMed ID21289174
'Rubinstein-Taybi syndrome (RSTS) is an inheritable disease associated with mutations in the gene encoding the CREB (cAMP response element-binding protein)-binding protein (CBP) and characterized by growth impairment, learning disabilities, and distinctive facial and skeletal features. Studies in mouse models for RSTS first suggested a direct role for CBP and histone ... More
A partial loss of function allele of methyl-CpG-binding protein 2 predicts a human neurodevelopmental syndrome.
AuthorsSamaco RC, Fryer JD, Ren J, Fyffe S, Chao HT, Sun Y, Greer JJ, Zoghbi HY, Neul JL
JournalHum Mol Genet
PubMed ID18321864
'Rett Syndrome, an X-linked dominant neurodevelopmental disorder characterized by regression of language and hand use, is primarily caused by mutations in methyl-CpG-binding protein 2 (MECP2). Loss of function mutations in MECP2 are also found in other neurodevelopmental disorders such as autism, Angelman-like syndrome and non-specific mental retardation. Furthermore, duplication ... More
Elevation of glutamine level by selenophosphate synthetase 1 knockdown induces megamitochondrial formation in Drosophila cells.
AuthorsShim MS, Kim JY, Jung HK, Lee KH, Xu XM, Carlson BA, Kim KW, Kim IY, Hatfield DL, Lee BJ
JournalJ Biol Chem
PubMed ID19755423
'Although selenophosphate synthetase 1 (SPS1/SelD) is an essential gene in Drosophila, its function has not been determined. To elucidate its intracellular role, we targeted the removal of SPS1/SelD mRNA in Drosophila SL2 cells using RNA interference technology that led to the formation of vacuole-like globular structures. Surprisingly, these structures ... More
Adaptation of Arabidopsis to nitrogen limitation involves induction of anthocyanin synthesis which is controlled by the NLA gene.
AuthorsPeng M, Hudson D, Schofield A, Tsao R, Yang R, Gu H, Bi YM, Rothstein SJ
JournalJ Exp Bot
PubMed ID18552353
'Plants can survive a limiting nitrogen (N) supply by developing a set of N limitation adaptive responses. However, the Arabidopsis nla (nitrogen limitation adaptation) mutant fails to produce such responses, and cannot adapt to N limitation. In this study, the nla mutant was utilized to understand further the ... More
Lack of Evidence for Green Tea Polyphenols as DNA Methylation Inhibitors in Murine Prostate
AuthorsKinney, SRM; Zhang, W; Pascual, M; Greally, JM; Gillard, BM; Karasik, E; Foster, BA; Karpf, AR
Journal
PubMed ID
'Green tea polyphenols (GTP) have been reported to inhibit DNA methylation in cultured cells. Here, we tested whether oral consumption of GTPs affects normal or cancer-specific DNA methylation in vivo, using mice. Wild-type (WT) and transgenic adenocarcinoma of mouse prostate (TRAMP) mice were given 0.3% GTPs in drinking water beginning ... More
Distinct risk factor profiles for human papillomavirus type 16-positive and human papillomavirus type 16-negative head and neck cancers.
AuthorsGillison ML, D'Souza G, Westra W, Sugar E, Xiao W, Begum S, Viscidi R
JournalJ Natl Cancer Inst
PubMed ID18334711
'BACKGROUND: High-risk types of human papillomavirus (HPV), including HPV-16, cause a subgroup of head and neck squamous cell carcinomas (HNSCCs). We examined whether the risk factors for HPV-16-positive HNSCCs are similar to those for HPV-16-negative HNSCCs in a hospital-based case-control study. METHODS: Case subjects (n = 240) diagnosed with ... More
Xenopus oocytes reactivate muscle gene transcription in transplanted somatic nuclei independently of myogenic factors.
AuthorsBiddle A, Simeoni I, Gurdon JB
JournalDevelopment
PubMed ID19605498
'Transplantation into eggs or oocytes is an effective means of achieving the reprogramming of somatic cell nuclei. We ask here whether the provision of gene-specific transcription factors forms part of the mechanism by which a gene that is repressed in somatic cells is transcribed in oocytes. We find that M1 ... More
Cloning and molecular characterization of R2R3-MYB and bHLH-MYC transcription factors from Citrus sinensis
AuthorsCultrone, A; Cotroneo, PS; Recupero, GR
Journal
PubMed ID
'Members of the MYB and MYC family regulate the biosynthesis of phenylpropanoids in several plant species. Two sequences, called CsMYB8 and CsMYC2, were identified from Citrus sinensis, and both the cDNA and the genomic clones were isolated and characterized from the flesh of common and blood oranges. Analysis by real-time ... More
Identification and analysis of differentially expressed genes in immune tissues of Atlantic cod stimulated with formalin-killed, atypical Aeromonas salmonicida.
AuthorsFeng CY, Johnson SC, Hori TS, Rise M, Hall JR, Gamperl AK, Hubert S, Kimball J, Bowman S, Rise ML
JournalPhysiol Genomics
PubMed ID19240301
'Physiological changes, elicited in animal immune tissues by exposure to pathogens, may be studied using functional genomics approaches. We created and characterized reciprocal suppression subtractive hybridization (SSH) cDNA libraries to identify differentially expressed genes in spleen and head kidney tissues of Atlantic cod (Gadus morhua) challenged with intraperitoneal injections of ... More
Abscisic acid regulates root hydraulic conductance via aquaporin expression modulation in Nicotiana tabacum
AuthorsMahdieh, M; Mostajeran, A
Journal
PubMed ID
'Abscisic acid (ABA) modifies the hydraulic properties of roots by increasing root water flux. The effects of ABA on aquaporin content and root hydraulic conductance are controversial. We addressed these effects via a combination of experiments. Tobacco (Nicotiana tabacum) plants were grown hydroponically, and ABA (1 μM) was exogenously applied ... More
Dietary blueberry attenuates whole-body insulin resistance in high fat-fed mice by reducing adipocyte death and its inflammatory sequelae.
'Adipose tissue (AT) inflammation promotes insulin resistance (IR) and other obesity complications. AT inflammation and IR are associated with oxidative stress, adipocyte death, and the scavenging of dead adipocytes by proinflammatory CD11c+ AT macrophages (ATMPhi). We tested the hypothesis that supplementation of an obesitogenic (high-fat) diet with whole blueberry ... More
Asporin and transforming growth factor-β gene expression in osteoblasts from subchondral bone and osteophytes in osteoarthritis
AuthorsSakao, K; Takahashi, KA; Arai, Y; Saito, M; Honjyo, K; Hiraoka, N; Kishida, T; Mazda, O; Imanishi, J; Kubo, T
Journal
PubMed ID
'To clarify the significance of subchondral bone and osteophytes in the pathology of osteoarthritis (OA), we investigated the expression of asporin (ASPN), transforming growth factor-β1 (TGF-β1), TGF-β2, TGF-β3, and runt-related transcription factor-2 (Runx2) genes involved in bone metabolism. Osteoblasts were isolated from 19 patients diagnosed with knee OA and from ... More
Ghrelin treatment causes increased food intake and retention of lean body mass in a rat model of cancer cachexia.
AuthorsDeBoer MD, Zhu XX, Levasseur P, Meguid MM, Suzuki S, Inui A, Taylor JE, Halem HA, Dong JZ, Datta R, Culler MD, Marks DL
JournalEndocrinology
PubMed ID17347304
'Cancer cachexia is a debilitating syndrome of anorexia and loss of lean body mass that accompanies many malignancies. Ghrelin is an orexigenic hormone with a short half-life that has been shown to improve food intake and weight gain in human and animal subjects with cancer cachexia. We used a rat ... More
Point mutation of a plastidic invertase inhibits development of the photosynthetic apparatus and enhances nitrate assimilation in sugar-treated Arabidopsis seedlings.
AuthorsTamoi M, Tabuchi T, Demuratani M, Otori K, Tanabe N, Maruta T, Shigeoka S
JournalJ Biol Chem
PubMed ID20304912
'Because the photosynthetic apparatus contains a massive amount of nitrogen in plants, the regulation of its development by sugar signals is important to the maintenance of the carbon-nitrogen balance. In this study we isolated an Arabidopsis mutant (sicy-192) whose cotyledon greening was inhibited by treatments with sugars such as sucrose, ... More
Glucose regulates ghrelin, neuropeptide Y, and the GH/IGF-I axis in the tilapia, Oreochromis mossambicus
'In general, a fish''s ability to clear glucose is sluggish in relation to mammals, which has lead to the idea that fish are glucose intolerant. It has been reported that circulating glucose levels do fluctuate in response to environmental challenges. Recent reports suggest that glucose may function as a metabolic ... More
AuthorsDanilova, OV; Tai, AK; Mele, DA; Beinborn, M; Leiter, AB; Greenberg, AS; Perfield, JW II; DeFuria, J; Singru, PS; Lechan, RM; Huber, BT
Journal
PubMed ID
'The control of glucose metabolism is a complex process, and dysregulation at any level can cause impaired glucose tolerance and insulin resistance. These two defects are well-known characteristics associated with obesity and onset of type 2 diabetes. Here we introduce the N-terminal dipeptidase, DPP2, as a novel regulator of the ... More
Cochlear pericyte responses to acoustic trauma and the involvement of hypoxia-inducible factor-1alpha and vascular endothelial growth factor.
AuthorsShi X
JournalAm J Pathol
PubMed ID19349367
'This study explored the effect of acoustic trauma on cochlear pericytes. Transmission electron microscopy revealed that pericytes on capillaries of the stria vascularis were closely associated with the endothelium in both control guinea pigs and mice. Pericyte foot processes were tightly positioned adjacent to endothelial cells. Exposure to wide-band noise ... More
Different secretion patterns of matrix metalloproteinases and IL-8 and effect of corticotropin-releasing hormone in preterm and term cervical fibroblasts.
AuthorsDubicke A, Akerud A, Sennstrom M, Hamad RR, Bystrom B, Malmstrom A, Ekman-Ordeberg G
JournalMol Hum Reprod
PubMed ID18922847
'The aims of the present study were to compare the levels of mRNA and protein expression of matrix metalloproteinase (MMP)-1, -3, -8 and -9 in human cervical tissue in preterm and term labor as well as not in labor and to determine if corticotropin-releasing hormone (CRH) has an effect on ... More
N-WASP is a novel regulator of hair-follicle cycling that controls antiproliferative TGFβ pathways
'N-WASP is a cytoplasmic molecule mediating Arp2/3 nucleated actin polymerization. Mice with a keratinocyte-specific deletion of the gene encoding N-WASP showed normal interfollicular epidermis, but delayed hair-follicle morphogenesis and abnormal hair-follicle cycling, associated with cyclic alopecia and prolonged catagen and telogen phases. The delayed anagen onset correlated with an increased ... More
Chronic enhancement of CREB activity in the hippocampus interferes with the retrieval of spatial information.
AuthorsViosca J, Malleret G, Bourtchouladze R, Benito E, Vronskava S, Kandel ER, Barco A
JournalLearn Mem
PubMed ID19237642
'The activation of cAMP-responsive element-binding protein (CREB)-dependent gene expression is thought to be critical for the formation of different types of long-term memory. To explore the consequences of chronic enhancement of CREB function on spatial memory in mammals, we examined spatial navigation in bitransgenic mice that express in a regulated ... More
Loss of prostaglandin E2 release from immortalized urothelial cells obtained from interstitial cystitis patient bladders.
AuthorsRastogi P, Rickard A, Dorokhov N, Klumpp DJ, McHowat J
JournalAm J Physiol Renal Physiol
PubMed ID18322019
'Interstitial cystitis (IC) is associated with increased activated mast cell numbers in the bladder and impairment of the barrier function of the urothelium. We stimulated immortalized urothelial cells derived from the inflamed region of IC bladders (SR22A or SM28 abn) or from healthy bladders (PD07i or PD08i) with tryptase ... More
Critical requirement of GABPalpha for normal T cell development.
AuthorsYu S, Zhao DM, Jothi R, Xue HH
JournalJ Biol Chem
PubMed ID20139079
'GA binding protein (GABP) consists of GABPalpha and GABPbeta subunits. GABPalpha is a member of Ets family transcription factors and binds DNA via its conserved Ets domain, whereas GABPbeta does not bind DNA but possesses transactivation activity. In T cells, GABP has been demonstrated to regulate the gene expression ... More
Steroidal androgens and nonsteroidal, tissue-selective androgen receptor modulator, S-22, regulate androgen receptor function through distinct genomic and nongenomic signaling pathways.
AuthorsNarayanan R, Coss CC, Yepuru M, Kearbey JD, Miller DD, Dalton JT
JournalMol Endocrinol
PubMed ID18801930
'Androgen receptor (AR) ligands are important for the development and function of several tissues and organs. However, the poor oral bioavailability, pharmacokinetic properties, and receptor cross-reactivity of testosterone, coupled with side effects, place limits on its clinical use. Selective AR modulators (SARMs) elicit anabolic effects in muscle and bone, ... More
Oxygen tension regulates pancreatic beta-cell differentiation through hypoxia-inducible factor 1alpha.
AuthorsHeinis M, Simon MT, Ilc K, Mazure NM, Pouyssegur J, Scharfmann R, Duvillie B
JournalDiabetes
PubMed ID20009089
'OBJECTIVE: Recent evidence indicates that low oxygen tension (pO2) or hypoxia controls the differentiation of several cell types during development. Variations of pO2 are mediated through the hypoxia-inducible factor (HIF), a crucial mediator of the adaptative response of cells to hypoxia. The aim of this study was to investigate the ... More
Lack of glucose recycling between endoplasmic reticulum and cytoplasm underlies cellular dysfunction in glucose-6-phosphatase-beta-deficient neutrophils in a congenital neutropenia syndrome.
AuthorsJun HS, Lee YM, Cheung YY, McDermott DH, Murphy PM, De Ravin SS, Mansfield BC, Chou JY
JournalOthers
PubMed ID20498302
'G6PC3 deficiency, characterized by neutropenia and neutrophil dysfunction, is caused by deficiencies in the endoplasmic reticulum (ER) enzyme glucose-6-phosphatase-beta (G6Pase-beta or G6PC3) that converts glucose-6-phosphate (G6P) into glucose, the primary energy source of neutrophils. Enhanced neutrophil ER stress and apoptosis underlie neutropenia in G6PC3 deficiency, but the exact functional role ... More
Pulmonary infection with an interferon-gamma-producing Cryptococcus neoformans strain results in classical macrophage activation and protection.
AuthorsHardison SE, Ravi S, Wozniak KL, Young ML, Olszewski MA, Wormley FL Jr
JournalAm J Pathol
PubMed ID20056835
'Alternative macrophage activation is associated with exacerbated disease in murine models of pulmonary cryptococcosis. The present study evaluated the efficacy of interferon-gamma transgene expression by Cryptococcus neoformans strain H99gamma in abrogating alternative macrophage activation in infected mice. Macrophage recruitment into the lungs of mice after infection with C. neoformans ... More
Association of MUTYH and MSH6 germline mutations in colorectal cancer patients
'Colorectal cancer (CRC) risk associated with germline monoallelic MUTYH mutations remains controversial, although a slightly increased risk for this disease has been suggested. MUTYH and MSH6 proteins act in cooperation during the DNA repair process. Based on this interaction, it was hypothesized that the combination of heterozygote germline mutations in ... More
Proteomic study on X-irradiation-responsive proteins and ageing: search for responsible proteins for radiation adaptive response.
AuthorsMiura Y, Kano M, Yamada M, Nishine T, Urano S, Suzuki S, Endo T, Toda T
JournalJ Biochem
PubMed ID17522089
'We investigated high- or low-dose irradiation-responsive proteins using proteomics on two-dimensional (2D) PAGE, and the effects of ageing on cell responses to radiation in variously aged rat astrocytes. After 5 Gy irradiation, the relative abundance of peroxiredoxin 2, an antioxidant enzyme, and latexin, an inhibitor of carboxypeptidase, increased. The ... More
Increased iron content and RNA oxidative damage in skeletal muscle with aging and disuse atrophy
AuthorsHofer, T; Marzetti, E; Xu, JZ; Seo, AY; Gulec, S; Knutson, MD; Leeuwenburgh, C; Dupont-Versteegden, EE
Journal
PubMed ID
'Muscle atrophy with aging or disuse is associated with deregulated iron homeostasis and increased oxidative stress likely inflicting damage to nucleic acids. Therefore, we investigated RNA and DNA oxidation, and iron homeostasis in gastrocnemius muscles. Disuse atrophy was induced in 6- and 32-month old male Fischer 344/Brown Norway rats by ... More
Adenylate cycalse toxin of Bordetella pertussis inhibits TLR-induced IRF-1 and IRF-8 activation and IL-12 production and enhances IL-10 through MAPK activation in dendritic cells.
AuthorsHickey FB, Brereton CF, Mills KH
JournalJ Leukoc Biol
PubMed ID18401006
'Adenylate cyclase toxin (CyaA) of Bordetella pertussis binds to CD11b/CD18 on macrophages and dendritic cells (DC) and confers virulence to the bacteria by subverting innate immune responses of the host. We have previously demonstrated that CyaA promotes the induction of IL-10-secreting regulatory T cells in vivo by modulating DC activation. ... More
Paradoxical effects of PDGF-BB overexpression in endothelial cells on engineered blood vessels in vivo.
AuthorsAu P, Tam J, Duda DG, Lin PC, Munn LL, Fukumura D, Jain RK
JournalAm J Pathol
PubMed ID19477947
'Therapeutic revascularization with either exogenous angiogenic growth factors or vascular cells has yet to demonstrate efficacy in the clinic. Injection of angiogenic growth factors often produces unstable and abnormal blood vessels. Blood vascular networks derived from implanted endothelial cells persist only transiently due to the insufficient recruitment of perivascular ... More
Regulation of islet beta-cell pyruvate metabolism: interactions of prolactin, glucose, and dexamethasone.
AuthorsArumugam R, Horowitz E, Noland RC, Lu D, Fleenor D, Freemark M
JournalEndocrinology
PubMed ID20484462
'Prolactin (PRL) induces beta-cell proliferation and glucose-stimulated insulin secretion (GSIS) and counteracts the effects of glucocorticoids on insulin production. The mechanisms by which PRL up-regulates GSIS are unknown. We used rat islets and insulinoma (INS-1) cells to explore the interactions of PRL, glucose, and dexamethasone (DEX) in the regulation of ... More
Triptolide induces pancreatic cancer cell death via inhibition of heat shock protein 70.
'Pancreatic cancer is highly resistant to current chemotherapy agents. We therefore examined the effects of triptolide (a diterpenoid triepoxide) on pancreatic cancer growth and local-regional tumor spread using an orthotopic model of pancreatic cancer. We have recently shown that an increased level of HSP70 in pancreatic cancer cells confers resistance ... More
Tonic B cell activation by Radioprotective105/MD-1 promotes disease progression in MRL/lpr mice.
AuthorsKobayashi T, Takahashi K, Nagai Y, Shibata T, Otani M, Izui S, Akira S, Gotoh Y, Kiyono H, Miyake K
JournalInt Immunol
PubMed ID18492657
'Toll-like receptors (TLRs) have a crucial role in sensing microbial products and triggering immune responses. Recent reports have indicated that TLR7 and TLR9 have an important role in activating autoreactive B cells. In addition to TLR7 and TLR9, mouse B cells express TLR2, TLR4 and structurally related Radioprotective105 (RP105). ... More
Novel Quinolone CHM-1 Induces Apoptosis and Inhibits Metastasis in a Human Osterogenic Sarcoma Cell Line
'Novel 2-phenyl-4-quinolone compounds have potent cytotoxic effects on different human cancer cell lines. In this study, we examined anticancer activity and mechanisms of 20-fluoro-6,7-methylenedioxy-2-phenyl-4-quinol one (CHM-1) in human osterogenic sarcoma U-2 OS cells. CHM-1-induced apoptosis was determined by flow cytometric analysis, DAPI staining, Comet assay, and caspase inhibitors. CHM-1-inhibited cell ... More
Promoter library designed for fine-tuned gene expression in Pichia pastoris.
AuthorsHartner FS, Ruth C, Langenegger D, Johnson SN, Hyka P, Lin-Cereghino GP, Lin-Cereghino J, Kovar K, Cregg JM, Glieder A
JournalNucleic Acids Res
PubMed ID18539608
'Although frequently used as protein production host, there is only a limited set of promoters available to drive the expression of recombinant proteins in Pichia pastoris. Fine-tuning of gene expression is often needed to maximize product yield and quality. However, for efficient knowledge-based engineering, a better understanding of ... More
MLL protects CpG clusters from methylation within the Hoxa9 gene, maintaining transcript expression.
'Homeobox (HOX) genes play a definitive role in determination of cell fate during embryogenesis and hematopoiesis. MLL-related leukemia is coincident with increased expression of a subset of HOX genes, including HOXA9. MLL functions to maintain, rather than initiate, expression of its target genes. However, the mechanism of MLL ... More
'Choline cytidylyltransferase (CCT) is the rate-limiting enzyme in the phosphatidylcholine biosynthetic pathway. Here, we demonstrate that CCTα-mediated phosphatidylcholine synthesis is required to maintain normal Golgi structure and function as well as cytokine secretion from the Golgi complex. CCTα is localized to the trans-Golgi region and its expression is increased in ... More
Arabidopsis BRANCHED1 acts as an integrator of branching signals within axillary buds.
AuthorsAguilar-Martinez JA, Poza-Carrion C, Cubas P
JournalPlant Cell
PubMed ID17307924
'Shoot branching patterns depend on a key developmental decision: whether axillary buds grow out to give a branch or whether they remain dormant in the axils of leaves. This decision is controlled by endogenous and environmental stimuli mediated by hormonal signals. Although genes involved in the long-distance signaling of this ... More
Tapetal cell fate, lineage and proliferation in the Arabidopsis anther.
AuthorsFeng X, Dickinson HG
JournalDevelopment
PubMed ID20570940
'The four microsporangia of the flowering plant anther develop from archesporial cells in the L2 of the primordium. Within each microsporangium, developing microsporocytes are surrounded by concentric monolayers of tapetal, middle layer and endothecial cells. How this intricate array of tissues, each containing relatively few cells, is established in an ... More
In vitro behavior of layer-by-layer deposited molecular oligoelectrolyte films on Ti-6Al-4V surfaces
'Layer-by-layer self-assembled films of molecular oligoelectrolytes were used to modify Ti-6Al-4V surfaces in order to test their ability as potential drug delivery system. With regard to medical application the in vitro behavior of the modified material was investigated. The Ti-6Al-4V (6% aluminium, 4% vanadium) material was treated in a layer-by-layer ... More
Hepatocyte-specific NEMO deletion promotes NK/NKT cell- and TRAIL-dependent liver damage.
AuthorsBeraza N, Malato Y, Sander LE, Al-Masaoudi M, Freimuth J, Riethmacher D, Gores GJ, Roskams T, Liedtke C, Trautwein C
JournalJ Exp Med
PubMed ID19635861
'Nuclear factor kappaB (NF-kappaB) is one of the main transcription factors involved in regulating apoptosis, inflammation, chronic liver disease, and cancer progression. The IKK complex mediates NF-kappaB activation and deletion of its regulatory subunit NEMO in hepatocytes (NEMO(Delta hepa)) triggers chronic inflammation and spontaneous hepatocellular carcinoma development. We show that ... More
Increased CXC ligand 10 levels and gene expression in type 1 leprosy reactions.
AuthorsScollard DM, Chaduvula MV, Martinez A, Fowlkes N, Nath I, Stryjewska BM, Kearney MT, Williams DL
JournalClin Vaccine Immunol
PubMed ID21508169
'Type 1 reaction (T1R) is a systemic inflammatory syndrome causing substantial morbidity in leprosy. T1R results from spontaneously enhanced cellular immunity in borderline types of leprosy, but there are no established laboratory markers for the reaction. Preliminary studies have identified elevated circulating CXC ligand 10 (CXCL10) during T1R. Correlation of ... More
Enumeration of Mycobacterium leprae using real-time PCR.
'Mycobacterium leprae is not cultivable in axenic media, and direct microscopic enumeration of the bacilli is complex, labor intensive, and suffers from limited sensitivity and specificity. We have developed a real-time PCR assay for quantifying M. leprae DNA in biological samples. Primers were identified to amplify a shared region ... More
Negative feedback regulation of UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis.
AuthorsGruber H, Heijde M, Heller W, Albert A, Seidlitz HK, Ulm R
JournalProc Natl Acad Sci U S A
PubMed ID21041653
'Plants respond to low levels of UV-B radiation with a coordinated photomorphogenic response that allows acclimation to this environmental stress factor. The key players in this UV-B response are COP1 (an E3 ubiquitin ligase), UVR8 (a beta-propeller protein), and HY5 (a bZIP transcription factor). We have shown previously that an ... More
The Fruitless gene in Nasonia displays complex sex-specific splicing and contains new zinc finger domains.
AuthorsBertossa RC, van de Zande L, Beukeboom LW
JournalMol Biol Evol
PubMed ID19349644
'The transcription factor Fruitless exerts a broad range of functions during Drosophila development, the most apparent of which is the determination of sexual behavior in males. Although fruitless sequences are found in other insect orders, little is known about fruitless structure and function outside Diptera. We have performed a thorough ... More
In Vivo Physiological Transdifferentiation of Adult Adipose Cells
'Grafts of adipose tissue from adult Rosa26 mice from different sites of the body, irrespective of the sex of the donor, share with the mammary fat the property of giving rise to milk-secreting epithelial cells when exposed to the microenvironment of the mammary gland in pregnant and lactating females. To ... More
The global regulator CodY regulates toxin gene expression in Bacillus anthracis and is required for full virulence.
Authorsvan Schaik W, Chateau A, Dillies MA, Coppee JY, Sonenshein AL, Fouet A
JournalInfect Immun
PubMed ID19651859
'In gram-positive bacteria, CodY is an important regulator of genes whose expression changes upon nutrient limitation and acts as a repressor of virulence gene expression in some pathogenic species. Here, we report the role of CodY in Bacillus anthracis, the etiologic agent of anthrax. Disruption of codY completely abolished ... More
IL-13 is required for eosinophil entry into the lung during respiratory syncytial virus vaccine-enhanced disease.
AuthorsCastilow EM, Meyerholz DK, Varga SM
JournalJ Immunol
PubMed ID18250447
'Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract disease in children. Children previously vaccinated with a formalin-inactivated RSV vaccine experienced enhanced morbidity and mortality upon natural RSV infection. Histological analysis revealed the presence of eosinophils in the pulmonary infiltrate of the vaccinated children. Eosinophils are characteristic ... More
Transactivation of lung lysozyme expression by Ets family member ESE-1.
AuthorsLei W, Jaramillo RJ, Harrod KS
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID17905856
'Epithelial-specific Ets (ESE) transcription factors, consisting of ESE-1, ESE-2, and ESE-3, are constitutively expressed in distinct epithelia of mucosal tissues, including the lung. Each ESE member exhibits alternative splicing and yields at least two isoforms (a and b) with transcriptional targets largely unidentified. The studies described herein define a ... More
Adenoviral delivery of interleukin-10 fails to attenuate experimental Lyme disease.
AuthorsBrown CR, Lai AY, Callen ST, Blaho VA, Hughes JM, Mitchell WJ
JournalInfect Immun
PubMed ID18824530
'Production of interleukin-10 (IL-10) by C57BL/6 mice following infection with Borrelia burgdorferi has been proposed as a mechanism whereby resistance to the development of experimental Lyme arthritis is maintained. In the current study, we sought to determine the role of IL-10 during infection of arthritis- and carditis-susceptible C3H mice. Infection ... More
Differential epigenetic regulation of Aiolos expression in human tumoral cell lines and primary cells
AuthorsDuhamel, M; Navarro, P; Cario-Andre, M; Billot, K; Arrouss, I; Rebollo, A
Journal
PubMed ID
'In order to investigate the epigenetic component of Aiolos regulation, we analyzed the methylation status of its 5'' CpG island in relation to historic modifications. Inhibition of CpG methylation restores Aiolos expression, as well as euchromatin-associated markers, in U937 and 1106 mel cell lines. DNA methylation and low levels of ... More
Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells.
AuthorsHaraguchi T, Ozaki Y, Iba H
JournalNucleic Acids Res
PubMed ID19223327
'Whereas the strong and stable suppression of specific microRNA activity would be essential for the functional analysis of these molecules, and also for the development of therapeutic applications, effective inhibitory methods to achieve this have not yet been fully established. In our current study, we tested various RNA ... More
A novel Entamoeba histolytica cysteine proteinase, EhCP4, is key for invasive amebiasis and a therapeutic target.
AuthorsHe C, Nora GP, Schneider EL, Kerr ID, Hansell E, Hirata K, Gonzalez D, Sajid M, Boyd SE, Hruz P, Cobo ER, Le C, Liu WT, Eckmann L, Dorrestein PC, Houpt ER, Brinen LS, Craik CS, Roush WR, McKerrow J, Reed SL
JournalJ Biol Chem
PubMed ID20378535
'Entamoeba histolytica cysteine proteinases (EhCPs) play a key role in disrupting the colonic epithelial barrier and the innate host immune response during invasion of E. histolytica, the protozoan cause of human amebiasis. EhCPs are encoded by 50 genes, of which ehcp4 (ehcp-a4) is the most up-regulated during invasion and ... More
Telomere attrition due to infection.
AuthorsIlmonen P, Kotrschal A, Penn DJ
JournalPlos One
PubMed ID18478110
'BACKGROUND: Telomeres--the terminal caps of chromosomes--become shorter as individuals age, and there is much interest in determining what causes telomere attrition since this process may play a role in biological aging. The leading hypothesis is that telomere attrition is due to inflammation, exposure to infectious agents, and other types of ... More
Effect of nandrolone decanoate administration on recovery from bupivacaine-induced muscle injury
AuthorsWhite, JP; Baltgalvis, KA; Sato, S; Wilson, LB; Carson, JA
Journal
PubMed ID
'White JP, Baltgalvis KA, Sato S, Wilson LB, Carson JA. Effect of nandrolone decanoate administration on recovery from bupivacaine-induced muscle injury. J Appl Physiol 107: 1420-1430, 2009. First published September 10, 2009; doi: 10.1152/japplphysiol.00668.2009.-Although testosterone administration elicits well-documented anabolic effects on skeletal muscle mass, the enhancement of muscle regeneration after ... More
Fasudil-induced hypoxia-inducible factor-1alpha degradation disrupts a hypoxia-driven vascular endothelial growth factor autocrine mechanism in endothelial cells.
'Hypoxic response of endothelial cells (EC) is an important component of tumor angiogenesis. Especially, hypoxia-inducible factor-1 (HIF-1)-dependent EC-specific mechanism is an essential component of tumor angiogenesis. Recently, the Rho/Rho-associated kinase (ROCK) signaling has been shown to play a key role in HIF-1alpha induction in renal cell carcinoma and ... More
Phospholipase d promotes lipid microdomain-associated signaling events in mast cells.
AuthorsLisboa FA, Peng Z, Combs CA, Beaven MA
JournalJ Immunol
PubMed ID19794068
'Initial IgE-dependent signaling events are associated with detergent-resistant membrane microdomains. Following Ag stimulation, the IgE-receptor (Fc(epsilon)RI ) accumulates within these domains. This facilitates the phosphorylation of Fc(epsilon)RI subunits by the Src kinase, Lyn, and the interaction with adaptor proteins, such as the linker for activation of T cells. Among ... More