AmpliTaq Gold™ 360 DNA Polymerase
Upgrade to Platinum II Taq Hot-Start DNA Polymerase for universal annealing and a 4X faster cycling protocol.
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AmpliTaq Gold™ 360 DNA Polymerase
Applied Biosystems™

AmpliTaq Gold™ 360 DNA Polymerase

Green features
The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.
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Catalog NumberQuantity
4398813100 Units
4398823250 Units
43988331000 Units
439889825 x 1000 Units
43988921500 Units
43988943000 Units
43988965000 Units
Catalog number 4398813
Price (USD)/ 100 units
151.65
Online exclusive
159.00 
Save 7.35 (5%)
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Add to cart
Quantity:
100 Units
Request bulk or custom format
Price (USD)/ 100 units
151.65
Online exclusive
159.00 
Save 7.35 (5%)
Add to cart
AmpliTaq Gold™ 360 DNA Polymerase
Catalog number4398813
Price (USD)/ 100 units
151.65
Online exclusive
159.00 
Save 7.35 (5%)
-
Add to cart
The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with AmpliTaq Gold 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. This polymerase efficiently amplifies targets present at low copy number, even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection and amplification of targets from degraded DNA samples. AmpliTaq Gold 360 DNA Polymerase delivers 360° coverage for a full range of targets.

AmpliTaq Gold 360 DNA Polymerase is purified by a proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes.

Features

  • Optimized for the broadest range of targets
  • High sensitivity, specificity, and yield
  • Robust amplification of GC-rich sequences with 360 GC Enhancer
  • High-quality sequencing data
  • Efficiently and reproducibly amplifies sequences up to 5 Kb

Optimized for easy and challenging targets

Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (see figure).

Optimized for hot-start PCR

AmpliTaq Gold 360 DNA Polymerase provides the same hot-start specificity as AmpliTaq Gold DNA Polymerase. A high-temperature incubation step is required to activate AmpliTaq Gold DNA Polymerase, which ensures that the active enzyme is generated only at temperatures in which the DNA is fully denatured and when the primers are not annealed.

When the polymerase is added to the reaction mixture at room temperature, primer extension does not occur because the enzyme is inactivated. Any low stringency mispriming events that may have occurred will not be enzymatically extended and will not be amplified. Hence, PCR setup can be performed at room temperature without concern for extension at misprimed sites. The amount of AmpliTaq Gold 360 DNA Polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers.

Notes

  • See figure below for a demonstration of robust PCR amplification of long human and plasmid DNA.
  • See user's manual or package insert for limited label license and trademark information.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatTube
GC-Rich PCR PerformanceHigh
PolymeraseAmpliTaq Gold 360 DNA Polymerase
Reaction SpeedStandard
Exonuclease Activity5' - 3'
Green FeaturesSustainable packaging
Product TypeDNA Polymerase
Quantity100 Units
Shipping ConditionRoom Temp or Wet Ice
For Use With (Application)Hot-start PCR
Concentration5 U/μL
Fidelity (vs. Taq)1X
Hot StartBuilt-In Hot Start
No. of Reactions80 Reactions
Overhang3'-A
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Starting MaterialDNA
Unit Size100 units
Contents & Storage
• AmpliTaq Gold 360 DNA Polymerase (5 U/μL), 1 x 20 μL
• 10X AmpliTaq Gold 360 Buffer, 1 x 1.5 mL
• 25 mM MgCl2, 1 x 1.5 mL
• 360 GC Enhancer, 1 x 1.5 mL

Store at -15°C to -25°C.