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Applied Biosystems™

AmpliTaq Gold™ 360 DNA Polymerase

Green features
Catalog number: 4398813
Applied Biosystems™

AmpliTaq Gold™ 360 DNA Polymerase

Green features
Catalog number: 4398813
Catalog Number
4398813
Unit Size
100 units
Price (USD)
135.00
Your Price:
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***
Quantity
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Unit Size
250 units
Price (USD)
332.00
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***
Quantity
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Unit Size
1,000 units
Price (USD)
1,158.00
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***
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1,500 units
Price (USD)
1,608.00
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5,000 units
Price (USD)
4,670.00
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3,000 units
Price (USD)
3,005.00
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25 x 1,000 units
Price (USD)
17,910.00
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
4398813100 units
135.00
Your Price:
***
4398823250 units
332.00
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43988331,000 units
1,158.00
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43988921,500 units
1,608.00
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43988965,000 units
4,670.00
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43988943,000 units
3,005.00
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439889825 x 1,000 units
17,910.00
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The Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold™ 360 DNA Polymerase delivers 360° coverage for a full range of targets.
* Optimized for the broadest range of targets—from everyday to challenging
* Unmatched sensitivity, specificity, and yield
* Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
* Achieves the highest quality sequencing data

Optimized for Easy and Challenging Targets

Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (Figure 1). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold™ 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Table 1).
As shown in Figure 1, GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.

Optimized for Hot-Start PCR

AmpliTaq Gold 360 DNA Polymerase provides the same hot-start specificity as AmpliTaq Gold DNA polymerase. A high-temperature incubation step is required to activate AmpliTaq Gold DNA Polymerase, which ensures that the active enzyme is generated only at temperatures in which the DNA is fully denatured and when the primers are not annealed.
When the polymerase is added to the reaction mixture at room temperature, primer extension does not occur because the enzyme is inactivated. Any low stringency mispriming events that may have occurred will not be enzymatically extended and will not be amplified. Hence, PCR setup can be performed at room temperature without concern for extension at misprimed sites. The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figure 2. The exquisite specificity allows easier multiplexing and allelic discrimination.
The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figures 2A & B. The exquisite specificity allows easier multiplexing and allelic discrimination.

Superior Sensitivity and Amplification Length

Compared to the original AmpliTaq Gold™ DNA Polymerase, AmpliTaq Gold™ 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes.
AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number (Figure 3), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, and amplification of targets from degraded DNA samples. The extreme purity of the enzyme contributes to its unmatched sensitivity. AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 Kb) sequences. Figure 4 demonstrates robust PCR amplification of long human and plasmid DNA.
 

Note:

See user's manual or package insert for limited label license, and trademark information.
For Research Use Only. Not for use in diagnostics procedures.

Specifications

Detection Method
Primer-Probe
Fidelity (vs. Taq)
1 X
GC-Rich PCR Performance
High
Hot Start
Built-In Hot Start
Overhang
3'-A
Polymerase
AmpliTaq Gold 360 DNA Polymerase
Reaction Format
Separate Components
Reaction Speed
Standard
Size (Final Product)
5 kb or less
Exonuclease Activity
5' - 3'
Green Features
Sustainable packaging
Product Line
AmpliTaq Gold™
Product Type
DNA Polymerase
Quantity
100 units
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice

Contents & Storage

• 1 x 100 U AmpliTaq Gold 360 DNA Polymerase
• 1 x 1.5 mL AmpliTaq Gold 360 Buffer, 10X
• 1 x 1.5 mL 25 mM MgCl2
• 1 x 1.5 mL 360 GC Enhancer

Store at -15 to -25°C.

Figures