Applied Biosystems™

Custom TaqMan™ Copy Number Assay This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  4400296

Related applications:

Real Time PCR (qPCR)

Back to top

Description

Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real-time PCR instruments and software. TaqMan Copy Number Assays are run together with a TaqMan Copy Number Reference Assay in a duplex qPCR reaction; the copy number assay detects the target sequence, and the reference assay detects a sequence that is known to be present in two copies in the diploid genome. The results are then analyzed by the relative quantitation method using Applied Biosystems CopyCaller Software.

Custom TaqMan Copy Number Assays are an option for targets of interest that are not covered by the predesigned assay collection. The GeneAssist Copy Number Assay Tool enables you to submit your own premasked custom target sequences for assay design or primer/probe pair sequences for assay formulation, at no extra charge. The design process for Custom TaqMan Copy Number Assays does not include up-front bioinformatic sequence analysis or in silico QC, but the designs can be compared with the human/mouse reference assays for compatibility in duplex reactions.

Benefits:
Flexible—generate assays for any target of interest using the GeneAssist Copy Number Assay Tool, with no species limitations
Specific—gold standard TaqMan chemistry with minor groove binder (MGB) probes increase assay specificity by enabling shorter probes
Reproducible—robust assay designs developed with our optimized algorithms deliver accurate, reproducible, and reliable results
Easy to interpret—CopyCaller Software provides the calculated copy number and predicted copy number, along with confidence value and z-score quality metrics
Fast and simple—setup to primary analysis in 3–4 hours

Approximate delivery time
4–6 days in North America and 6–10 days in Europe

TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

TaqMan Copy Number Reference Assays are sold separately.

Recommended master mix (sold separately): TaqMan Genotyping Master Mix

For Research Use Only. Not for use in diagnostic procedures.

Specifications

CTA Type: Magellan_PDP_OrderNow
Green Features: Sustainable packaging
Product Line: TaqMan™
Product Size: L (2900 reactions), made to order
Shipping Condition: Room Temperature

Contents & storage

1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.