Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real-time PCR instruments and software. TaqMan Copy Number Assays are run together with a TaqMan Copy Number Reference Assay in a duplex qPCR reaction; the copy number assay detects the target sequence, and the reference assay detects a sequence that is known to be present in two copies in the diploid genome. The results are then analyzed by the relative quantitation method using Applied Biosystems CopyCaller Software.

Custom TaqMan Copy Number Assays are an option for targets of interest that are not covered by the predesigned assay collection. The GeneAssist Copy Number Assay Tool enables you to submit your own premasked custom target sequences for assay design or primer/probe pair sequences for assay formulation, at no extra charge. The design process for Custom TaqMan Copy Number Assays does not include up-front bioinformatic sequence analysis or in silico QC, but the designs can be compared with the human/mouse reference assays for compatibility in duplex reactions.

Benefits:
• Flexible—generate assays for any target of interest using the GeneAssist Copy Number Assay Tool, with no species limitations
• Specific—gold standard TaqMan chemistry with minor groove binder (MGB) probes increase assay specificity by enabling shorter probes
• Reproducible—robust assay designs developed with our optimized algorithms deliver accurate, reproducible, and reliable results
• Easy to interpret—CopyCaller Software provides the calculated copy number and predicted copy number, along with confidence value and z-score quality metrics
• Fast and simple—setup to primary analysis in 3–4 hours

Approximate delivery time
4–6 days in North America and 6–10 days in Europe

TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

TaqMan Copy Number Reference Assays are sold separately.

Recommended master mix (sold separately): TaqMan Genotyping Master Mix