BACKGROUND AND AIM: Methylated SEPT9 is a novel circulating tumor DNA marker for colorectal cancer, while the effects of various colorectal cancer clinicopathological factors on its detection performance have not been fully evaluated. This study aims to investigate the significance of the clinicopathological factors on methylated SEPT9 performance in a ... More
mTORC1/2 inhibition re-sensitizes platinum-resistant ovarian cancer by disrupting selective translation of DNA damage and survival mRNAs.
AuthorsDavid-West G,Ernlund A,Gadi A,Schneider RJ
JournalOncotarget
PubMed ID30237852
Platinum resistance is a major cause of treatment failure and mortality in epithelial ovarian cancer. mTORC1/2 inhibitors, which impair mRNA translation, can re-sensitize resistant ovarian cancer cells to platinum chemotherapy but the mechanism remains poorly described. Using platinum-resistant OVCAR-3 cells treated with the selective mTORC1/2 inhibitor INK128/MLN128, we conducted genome-wide ... More
Role of the vacuolar ATPase in the Alphavirus replication cycle.
We have shown that Alphaviruses can enter cells by direct penetration at the plasma membrane (R. Vancini, G. Wang, D. Ferreira, R. Hernandez, and D. Brown, J Virol, 87:4352â4359, 2013). Direct penetration removes the requirement for receptor-mediated endocytosis exposure to low pH and membrane fusion in the process of RNA ... More
Expression profiling identified IL-8 as a regulator of homotypic cell-in-cell formation.
Homotypic cell-in-cell (CIC) structures forming between cancer cells were proposed to promote tumor evolution via entosis, a nonapoptotic cell death process. However, the mechanisms underlying their formation remained poorly understood. We performed a microarray analysis to identify genes associated with homotypic CIC formation. Cancer cells differing in their ability to ... More
Validation of a Host Response Assay, SeptiCyte LAB, for Discriminating Sepsis from Systemic Inflammatory Response Syndrome in the ICU.
JournalAmerican journal of respiratory and critical care medicine
PubMed ID29624409
Rationale: A molecular test to distinguish between sepsis and systemic inflammation of noninfectious etiology could potentially have clinical utility. Objectives: This study evaluated the diagnostic performance of a molecular host response assay (SeptiCyte LAB) designed to distinguish between sepsis and noninfectious systemic inflammation in critically ill adults. Methods: The study ... More
Molecular and functional interaction between GPR18 and cannabinoid CB(2) G-protein-coupled receptors. Relevance in neurodegenerative diseases.
BACKGROUND: Due to the high rate of antigenic variation of influenza virus, seasonal characterization of the virus is crucial to assess and monitor the emergence of new pathogenic variants and hence formulate effective control measures. However, no study has yet been conducted in Mozambique to assess genetic, antigenic and antiviral ... More
Enhanced throughput of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) real-time RT-PCR panel by assay multiplexing and specimen pooling.
AuthorsLu X,Sakthivel SK,Wang L,Lynch B,Dollard SM
JournalJournal of virological methods
PubMed ID33839185
A multiplex real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) assay for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was developed based on the same primer and probe sequences of an existing U.S. CDC Emergency Use authorized test panel, targeting SARS-CoV-2 N1, N2 and human RNase P genes in singleplex. ... More
Heterozygous Recurrent Mutations Inducing Dysfunction of ROR2 Gene in Patients With Short Stature.
JournalFrontiers in cell and developmental biology
PubMed ID33937263
PURPOSE: ROR2, a member of the ROR family, is essential for skeletal development as a receptor of Wnt5a. The present study aims to investigate the mutational spectrum of ROR2 in children with short stature and to identify the underlying molecular mechanisms. METHODS: We retrospectively analyzed clinical phenotype and whole-exome sequencing ... More
Wastewater surveillance-based city zonation for effective COVID-19 pandemic preparedness powered by early warning: A perspectives of temporal variations in SARS-CoV-2-RNA in Ahmedabad, India.
AuthorsKumar M,Joshi M,Shah AV,Srivastava V,Dave S
JournalThe Science of the total environment
PubMed ID34465041
Following the proven concept, capabilities, and limitations of detecting the RNA of Severe Acute Respiratory Coronavirus 2 (SARS-CoV-2) in wastewater, it is pertinent to understand the utility of wastewater surveillance data on various scale. In the present work, we put forward the first wastewater surveillance-based city zonation for effective COVID-19 ... More
hsa‑miR‑5580‑3p inhibits oral cancer cell viability, proliferation and migration by suppressing LAMC2.
AuthorsFang R,Lu Q,Xu B
JournalMolecular medicine reports
PubMed ID33880581
The present study aimed to explore whether and how microRNA-5580-3p (miR-5580-3p) affected oral cancer (OC) cell phenotypes via regulation of laminin subunit γ2 (LAMC2). Bioinformatics analysis was used to identify miR-5580-3p/LAMC2, a novel interactome that, to the best of our knowledge, has not been studied previously in OC. In the ... More
Duplex real-time RT-PCR assay for detection and subgroup-specific identification of human respiratory syncytial virus.
Human respiratory syncytial virus (HRSV) is a leading cause of acute respiratory illness in young children worldwide. Reliable detection and identification of HRSV subgroup A and B infections are essential for accurate disease burden estimates in anticipation of licensure of novel HRSV vaccines and immunotherapies. To ensure continued reliability, molecular ... More
Antidrug resistance in the Indian ambient waters of Ahmedabad during the COVID-19 pandemic.
The ongoing COVID-19 pandemic increases the consumption of antimicrobial substances (ABS) due to the unavailability of approved vaccine(s). To assess the effect of imprudent consumption of ABS during the COVID-19 pandemic, we compare the 2020 prevalence of antidrug resistance (ADR) of Escherichia coli (E. coli) with a similar survey carried ... More
Multiplex Real-Time Reverse Transcription PCR for Influenza A Virus, Influenza B Virus, and Severe Acute Respiratory Syndrome Coronavirus 2.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in late 2019, and the outbreak rapidly evolved into the current coronavirus disease pandemic. SARS-CoV-2 is a respiratory virus that causes symptoms similar to those caused by influenza A and B viruses. On July 2, 2020, the US Food and Drug Administration ... More
Triptolide inhibits angiogenesis in microvascular endothelial cells through regulation of miR-92a.
AuthorsXu X,Tian L,Zhang Z
JournalJournal of physiology and biochemistry
PubMed ID31691162
Microbial Source Tracking Approach to Investigate Fecal Waste at the Strawberry Creek Watershed and Clam Beach, California, USA.
AuthorsCorrigan JA,Butkus SR,Ferris ME,Roberts JC
JournalInternational journal of environmental research and public health
PubMed ID34199071
Clam Beach is located in Northern California, USA, and is listed as an impaired waterway by the federal government. The scope of this study was to investigate this beach and surrounding watershed to determine, if possible, the source of the impairment by conducting an 11-h beach study and 8-week watershed ... More
PARP1 Suppresses the Transcription of PD-L1 by Poly(ADP-Ribosyl)ating STAT3.
Exosomal Circular RNA as a Biomarker Platform for the Early Diagnosis of Immune-Mediated Demyelinating Disease.
AuthorsHe J,Ren M,Li H,Yang L,Wang X,Yang Q
JournalFrontiers in genetics
PubMed ID31611906
Exosomes can pass through the blood-brain barrier and are present in the cerebrospinal fluid (CSF). The components in exosomes, such as DNA, RNA, protein, and lipids, change greatly and are closely related to disease progression. Circular RNA (circRNA) is stable in structure and has a long half-life in exosomes without ... More
Circulating follicular helper T cells are increased in systemic sclerosis and promote plasmablast differentiation through the IL-21 pathway which can be inhibited by ruxolitinib.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious and has caused significant medical/socioeconomic impacts. Other than vaccination, effective public health measures, including contact tracing, isolation, and quarantine, is critical for deterring viral transmission, preventing infection progression and resuming normal activities. Viral transmission is affected by many factors, but ... More
V348I mutation in UL23 gene of human herpesvirus 1 in a case of herpetic hepatitis and haemophagocytic lymphohistiocytosis.
AuthorsCorcione S,Sidoti F,Costa C,Mornese Pinna S,Bonetto C,Urbino R,Cavallo R,De Rosa FG
JournalNew microbes and new infections
PubMed ID30988955
We herein report the case of a young immunocompetent adult patient with a rapidly fatal haemophagocytic lymphohistiocytosis syndrome related to human herpesvirus 1 (HHV-1) infection, with herpetic hepatitis and persistent high-level viraemia despite treatment with acyclovir. Haemophagocytic lymphohistiocytosis was confirmed in the patient's spleen and bone marrow. HHV-1 DNA was ... More
Development and evaluation of a novel Campylobacter spp. enrichment medium.
AuthorsAl S,Incili GK,Akcay A,Koluman A
JournalJournal of microbiological methods
PubMed ID30641093
Performance Evaluation of Serial SARS-CoV-2 Rapid Antigen Testing During a Nursing Home Outbreak.
AuthorsMcKay SL,Tobolowsky FA,Moritz ED,Hatfield KM,Bhatnagar A,LaVoie SP,Jackson DA,Lecy KD,Bryant-Genevier J,Campbell D,Freeman B,Gilbert SE,Folster JM,Medrzycki M,Shewmaker PL,Bankamp B,Radford KW,Anderson R,Bowen MD,Negley J,Reddy SC,Jernigan JA,Brown AC,McDonald LC,Kutty PK,CDC Infection Prevention and Control Team and the CDC COVID-19 Surge Laboratory Group
JournalAnnals of internal medicine
PubMed ID33900791
BACKGROUND: To address high COVID-19 burden in U.S. nursing homes, rapid SARS-CoV-2 antigen tests have been widely distributed in those facilities. However, performance data are lacking, especially in asymptomatic people. OBJECTIVE: To evaluate the performance of SARS-CoV-2 antigen testing when used for facility-wide testing during a nursing home outbreak. DESIGN: ... More
A high-cholesterol diet promotes steatohepatitis and liver tumorigenesis in HCV core gene transgenic mice.
Cost Effectiveness of Sample Pooling to Test for SARS-CoV-2.
AuthorsAbdalhamid B,Bilder CR,Garrett JL,Iwen PC
JournalJournal of infection in developing countries
PubMed ID33175708
Genome sequencing of SARS-CoV-2 in a cohort of Egyptian patients revealed mutation hotspots that are related to clinical outcomes.
AuthorsZekri AN,Mohanad M,Hafez MM,Soliman HK,Hassan ZK,Abouelhoda M,Amer KE,Seadawy MG,Ahmed OS
JournalBiochimica et biophysica acta. Molecular basis of disease
PubMed ID33932525
BACKGROUND: Severe acute respiratory syndrome-2 (SARS-CoV-2) exhibits a broad spectrum of clinical manifestations. Despite the fact that SARS-CoV-2 has slower evolutionary rate than other coronaviruses, different mutational hotspots have been identified along the SARS-CoV-2 genome. METHODS: We performed whole-genome high throughput sequencing on isolates from 50 Egyptian patients to see ... More
SMARCC1 Suppresses Tumor Progression by Inhibiting the PI3K/AKT Signaling Pathway in Prostate Cancer.
JournalFrontiers in cell and developmental biology
PubMed ID34249931
BACKGROUND: SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily C member 1 (SMARCC1) protein is a potential tumor suppressor in various cancers. However, its role in prostate cancer (PCa) remains controversial. The aim of this study was to determine the biological function of SMARCC1 in PCa and explore the underlying regulatory ... More
The relationship between MMP9 and ADRA2A gene polymorphisms and mothers-newborns' nutritional status: an exploratory path model (STROBE compliant article).
AuthorsMărginean CO,Mărginean C,Bănescu C,Meliţ LE,Tripon F,Iancu M
JournalPediatric research
PubMed ID30791043
BACKGROUND: The aim of this study was to evaluate the direct effects of matrix metalloproteinase (MMP9 rs17577, MMP9 rs17576) and alfa 2 adrenergic receptor (ADRA2A rs553668) gene polymorphisms investigated in mothers and their newborns on maternal weight gain (MWG) during pregnancy and the newbornâs birth weight (BW), taking into account ... More
Gene Expression Profile of Vascular Endothelial Growth Factors (VEGFs) and Platelet-derived Growth Factors (PDGFs) in the Normal Cornea.
AuthorsDan Cosnita AR,Raica M,Sava MP,Cimpean AM
JournalIn vivo (Athens, Greece)
PubMed ID33622873
Background/Aim: Angiogenic growth factors expression is not known in the normal cornea. The aim was to study corneal gene expression profile of VEGF and PDGF pathways influencing the avascular state of cornea. Materials and Methods: cDNA synthesis was performed from mRNA extracted from five fresh pig corneas followed by cDNA ... More
Blueprint for a pop-up SARS-CoV-2 testing lab.
AuthorsIGI Testing Consortium
JournalNature biotechnology
PubMed ID32555529
Overexpression of GmGAMYB Accelerates the Transition to Flowering and Increases Plant Height in Soybean.
AuthorsYang X,Li X,Shan J,Li Y,Zhang Y,Wang Y,Li W,Zhao L
JournalFrontiers in plant science
PubMed ID34040624
The flowering time and plant height of soybean are important agronomic characters, which control the adaptability and yield of soybean. R2R3 MYB transcription factor plays an important regulatory role in plant growth and development. In this study, soybean GmGAMYB gene of R2R3-MYB type was induced by long-days (LDs). GmGAMYB showed ... More
Molecular detection of enterovirus D68 among children with acute respiratory tract infection in Ghana.
AuthorsKubi JA,Mutocheluh M,Bonney JHK,Ampofo WK,Odoom JK
JournalAfrican journal of laboratory medicine
PubMed ID31309045
BACKGROUND: Acute respiratory tract infections of viral origin remain a leading cause of morbidity, mortality and economic loss regardless of age or gender. A small number of acute respiratory tract infection cases caused by enterovirus D68 (EV-D68) have been reported regularly to Centers for Disease Control and Prevention since 1987 ... More
LncRNA NEAT1 Acts as an miR-148b-3p Sponge to Regulate ROCK1 Inhibition of Retinoblastoma Growth.
AuthorsLu H,Zhang Z,Lu Y,Xiu W,Cui J
JournalCancer management and research
PubMed ID34285579
BACKGROUND: It is reported that long non-coding RNA nuclear paraspeckle assembly transcript 1 (LncRNA NEAT1) is involved in the occurrence and development of various cancers. However, the detailed biological function and mechanism of LncRNA NEAT1 in retinoblastoma are still unclear. So we will explore the biological function and possible mechanism ... More
Evaluation of a High-Definition PCR Assay for the Detection of SARS-CoV-2 in Extracted and Nonextracted Respiratory Specimens Collected in Various Transport Media.
AuthorsBuchan BW,Gerstbrein D,Cruz A,Hoff J,Sievert E,Ledeboer NA,Faron ML
JournalAmerican journal of clinical pathology
PubMed ID33940605
OBJECTIVES: We conducted an analytic and clinical comparison of a novel high-definition polymerase chain reaction PCR (HDPCR) assay to traditional real-time PCR (RT-PCR) for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in upper respiratory specimens. METHODS: Analytic performance of RT-PCR, HDPCR, and extraction-free HDPCR was established through ... More
Vaccine Breakthrough Infections with SARS-CoV-2 Variants.
Emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are of clinical concern. In a cohort of 417 persons who had received the second dose of BNT162b2 (PfizerâBioNTech) or mRNA-1273 (Moderna) vaccine at least 2 weeks previously, we identified 2 women with vaccine breakthrough infection. Despite evidence of vaccine ... More
Reciprocal regulation of miR-206 and IL-6/STAT3 pathway mediates IL6-induced gefitinib resistance in EGFR-mutant lung cancer cells.
AuthorsYang Y,Wang W,Chang H,Han Z,Yu X,Zhang T
JournalJournal of cellular and molecular medicine
PubMed ID31507089
Persistently activated ILâ6/STAT3 pathway promotes acquired resistance to targeted therapy with epidermal growth factor receptorâtyrosine kinase inhibitors (EGFRâTKIs) in nonâsmallâcell lung cancer (NSCLC) treatment. miRâ206 has been verified to be dysregulated and plays as a negative regulator in lung cancer. However, whether miRâ206 may overcome IL6âinduced gefitinib resistance in EGFRâmutant ... More
Post-exposure immunotherapy for two ebolaviruses and Marburg virus in nonhuman primates.
The 2013â2016 Ebola virus (EBOV) disease epidemic demonstrated the grave consequences of filovirus epidemics in the absence of effective therapeutics. Besides EBOV, two additional ebolaviruses, Sudan (SUDV) and Bundibugyo (BDBV) viruses, as well as multiple variants of Marburg virus (MARV), have also caused high fatality epidemics. Current experimental EBOV monoclonal ... More
A rapid near-patient detection system for SARS-CoV-2 using saliva.
The highly infectious nature of SARS-CoV-2 necessitates the use of widespread testing to control the spread of the virus. Presently, the standard molecular testing method (reverse transcriptase-polymerase chain reaction, RT-PCR) is restricted to the laboratory, time-consuming, and costly. This increases the turnaround time for getting test results. This study sought ... More
Comparative evaluation of the Thermo fisher TaqPath™ COVID-19 combo kit with the Cepheid Xpert® Xpress SARS-CoV-2 assay for detecting SARS-CoV-2 in nasopharyngeal specimens.
AuthorsGranato PA,Kimball SR,Alkins BR,Cross DC,Unz MM
JournalBMC infectious diseases
PubMed ID34193072
PURPOSE: With over 50 SARS-CoV-2 gene amplification assays that have been EUA cleared with minimal experimental validation performed, it is likely that not all of these assays are comparable in their ability to detect SARS-CoV-2 in clinical specimens. Thermo Fisher Scientific is a relatively new company in the molecular diagnostics ... More
Unravelling the early warning capability of wastewater surveillance for COVID-19: A temporal study on SARS-CoV-2 RNA detection and need for the escalation.
AuthorsKumar M,Joshi M,Patel AK,Joshi CG
JournalEnvironmental research
PubMed ID33662347
Wastewater-based Epidemiological (WBE) surveillance offers a promising approach to assess the pandemic situation covering pre-symptomatic and asymptomatic cases in highly populated area under limited clinical tests. In the present study, we analyzed SARS-CoV-2 RNA in the influent wastewater samples (n = 43) from four wastewater treatment plants (WWTPs) in Gandhinagar, India, during ... More
Evaluation of a Novel High-Definition PCR Multiplex Assay for Simultaneous Detection of Tick-Borne Pathogens in Human Clinical Specimens.
AuthorsShakir SM,Mansfield CR,Hays ED,Couturier MR,Hillyard DR
JournalJournal of clinical microbiology
PubMed ID31852765
The incidence of tick-borne infections in the United States has risen significantly in the past decade. Ticks can transmit a variety of pathogens, including bacteria, protozoa, and viruses, that can cause serious illnesses. Therefore, the use of rapid, sensitive, and specific multiplex tests is important to identify the pathogen(s) in ... More
First comparison of conventional activated sludge versus root-zone treatment for SARS-CoV-2 RNA removal from wastewaters: Statistical and temporal significance.
AuthorsKumar M,Kuroda K,Joshi M,Bhattacharya P,Barcelo D
In the initial pandemic phase, effluents from wastewater treatment facilities were reported mostly free from Severe Acute Respiratory Coronavirus 2 (SARS-CoV-2) RNA, and thus conventional wastewater treatments were generally considered effective. However, there is a lack of first-hand data on i) comparative efficacy of various treatment processes for SARS-CoV-2 RNA ... More
SARS-CoV2 infection impairs the metabolism and redox function of cellular glutathione.
Viral infections sustain their replication cycle promoting a pro-oxidant environment in the host cell. In this context, specific alterations of the levels and homeostatic function of the tripeptide glutathione have been reported to play a causal role in the pro-oxidant and cytopathic effects (CPE) of the virus. In this study, ... More
Prevalence of SARS-CoV-2 infection and dynamics of antibodies response among previously undiagnosed healthcare workers in a university hospital: A prospective cohort study.
BACKGROUND: Healthcare workers (HCWs) are a presumed high-risk population for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Identifying factors associated with seroprevalence can help establish better practices in healthcare settings. In this study, we evaluate prevalence of SARS-CoV-2 infection among previously undiagnosed HCWs and describe profiling of antibody responses against ... More
Associations of homocysteine status and homocysteine metabolism enzyme polymorphisms with hypertension and dyslipidemia in a Chinese hypertensive population.
JournalClinical and experimental hypertension (New York, N.Y. : 1993)
PubMed ID30786773
MC4R and ENPP1 gene polymorphisms and their implication in maternal and neonatal risk for obesity.
AuthorsMărginean C,Mărginean CO,Iancu M,Meliț LE,Tripon F,Bănescu C
JournalScientific reports
PubMed ID31350533
The aims of this study were to establish the role of MC4Rrs17782313 and ENPP1rs1044498 gene polymorphisms on pre-pregnancy BMI and the newbornâs status. We performed a cross-sectional study on 185 mothers and their offspring. The groups were divided into: control group- underweight or normal mothers with BMI(initial)â<â25âkg/m(2) (n(1)â=â134) and study ... More
The mSHOX2 is capable of assessing the therapeutic effect and predicting the prognosis of stage IV lung cancer.
AuthorsPeng X,Liu X,Xu L,Li Y,Wang H,Song L,Xiao W
JournalJournal of thoracic disease
PubMed ID31372283
BACKGROUND: Instant monitoring of the therapeutic effect of systematic therapy in late-stage lung cancer is crucial for response assessment and strategy adjustment. Previous study found that specific plasma methylation markers may be applied to therapeutic effect assessment. In order to investigate the performance of plasma mSHOX2 in assessing the therapeutic ... More
A pilot study of a novel molecular host response assay to diagnose infection in patients after high-risk gastro-intestinal surgery.
Background: The complex interactions that exist between the pacemaker current, I (f), and the parasympathetic nervous system could significantly influence the course of patients undergoing chronic therapy with the I (f) blocker ivabradine. We thus aimed to assess the effects of chronic ivabradine therapy on autonomic modulation and on the ... More
Identification of a complex genomic rearrangement in TMPRSS3 by massively parallel sequencing in Chinese cases with prelingual hearing loss.
BACKGROUND: Genetic variants in TMPRSS3 have been causally linked to autosomal recessive nonsyndromic hearing loss (HL) at the DFNB8 and DFNB10 loci. These variants include both single nucleotide and copy number variations (CNVs). In this study, we aim to identify the genetic cause in three Chinese subjects with prelingual profound ... More
Effect of sevoflurane treatment on microglia activation, NF-kB and MAPK activities.
AuthorsYu X,Zhang F,Shi J
JournalImmunobiology
PubMed ID31451204
Long non-coding RNA GAS5 sensitizes renal cell carcinoma to sorafenib via miR-21/SOX5 pathway.
AuthorsLiu L,Pang X,Shang W,Xie H,Feng Y,Feng G
JournalCell cycle (Georgetown, Tex.)
PubMed ID29895198
Although the use of sorafenib appears to increase the survival rate of renal cell carcinoma (RCC) patients, there is also a proportion of patients who exhibit a poor primary response to sorafenib treatment. Therefore, it is critical to elucidate the mechanisms underlying sorafenib resistance and find representative biomarkers for sorafenib ... More
Assessment of eight nucleic acid amplification technologies for potential use to detect infectious agents in low-resource settings.
Nucleic acid amplification technologies (NAATs) are high-performance tools for rapidly and accurately detecting infectious agents. They are widely used in high-income countries to diagnose disease and improve patient care. The complexities associated with test methods, reagents, equipment, quality control and assurance require dedicated laboratories with trained staff, which can exclude ... More
Curcumin mediates reversion of HGF-induced epithelial-mesenchymal transition via inhibition of c-Met expression in DU145 cells.
AuthorsHu HJ,Lin XL,Liu MH,Fan XJ,Zou WW
JournalOncology letters
PubMed ID26893768
The hepatocyte growth factor (HGF)/c-Met signaling pathway results in cancer cell scattering and invasion, and has been reported to participate in several types of cancer, including prostate and colorectal cancer. The downstream phosphorylation cascade of HGF, particularly the mitogen-activated protein kinase and phosphoinositide 3-kinase/AKT signaling pathway, regulates epithelial-mesenchymal transition (EMT). ... More
One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples.
AuthorsAlm E,Lindegren G,Falk KI,Lagerqvist N
JournalBMC infectious diseases
PubMed ID26527283
BACKGROUND: Dengue is one of the leading causes of morbidity in tropical and subtropical regions and infection with any of the four dengue virus serotypes (DENV1-4) result in a wide range of clinical manifestations. Given the geographic expansion of DENV1-4, assays for serotyping are needed to be able to perform ... More
Long-term stabilization of DNA at room temperature using a one-step microwave assisted process.
AuthorsNarvaez Villarrubia CW,Tumas KC,Chauhan R,MacDonald T,Dattelbaum AM,Omberg K,Gupta G
JournalEmergent materials
PubMed ID33778372
Long-term stabilization of DNA is needed for forensic, clinical, in-field operations and numerous other applications. Although freezing (<â20 °C) and dry storage are currently the preferential methods for long-term storage, a noticeable pre-analytical degradation of DNA over time, upfront capital investment and recurring costs have demonstrated a need for an ... More
Algorithm Optimization in Methylation Detection with Multiple RT-qPCR.
Epigenetic markers based on differential methylation of DNA sequences are used in cancer screening and diagnostics. Detection of abnormal methylation at specific loci by real-time quantitative polymerase chain reaction (RT-qPCR) has been developed to enable high-throughput cancer screening. For tests that combine the results of multiple PCR replicates into a ... More
DNA-PK inhibitor peposertib enhances p53-dependent cytotoxicity of DNA double-strand break inducing therapy in acute leukemia.
Peposertib (M3814) is a potent and selective DNA-PK inhibitor in early clinical development. It effectively blocks non-homologous end-joining repair of DNA double-strand breaks (DSB) and strongly potentiates the antitumor effect of ionizing radiation (IR) and topoisomerase II inhibitors. By suppressing DNA-PK catalytic activity in the presence of DNA DSB, M3814 ... More
In vivo, ex vivo and in vitro dasatinib activity in chronic lymphocytic leukemia.
Dasatinib inhibits the breakpoint cluster region-Abelson murine leukemia 1 (BCR-ABL1) gene along with other kinases known to be overexpressed and abnormally active in patients with chronic lymphocytic leukemia (CLL). The current study used primary leukemic cells obtained from 53 patients with CLL that were treated with dasatinib. A 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) ... More
Usability of an At-Home Anterior Nares SARS-CoV-2 RT-PCR Sample Collection Kit: Human Factors Feasibility Study.
AuthorsStrong LE,Middendorf I,Turner M,Edwards V DK,Sama V,Mou J,Adams KC
JournalJMIR human factors
PubMed ID34609947
BACKGROUND: Readily available testing for SARS-CoV-2 is necessary to mitigate COVID-19 disease outbreaks. At-home collection kits, in which samples are self-collected without requiring a laboratory or clinic visit and sent to an external laboratory for testing, can provide convenient testing to those with barriers to access. They can prevent unnecessary ... More
"Double-duty" conventional dendritic cells in the amphibian Xenopus as the prototype for antigen presentation to B cells.
Two populations of dendritic cells (DCs) are found in mammals, one derived from hematopoietic precursors (conventional/cDC), and another derived from mesenchymal precursors, the follicular DC (FDC); the latter is specialized for antigen presentation to B cells, and has only been definitively demonstrated in mammals. Both cDC and FDC are necessary ... More
LncRNA NEAT1 promotes endometrial cancer cell proliferation, migration and invasion by regulating the miR-144-3p/EZH2 axis.
BACKGROUND: Endometrial cancer (EC) is one of the most common gynaecological tumours in the worldwide. Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes cell proliferation, migration and invasion in EC cells. However, the molecular mechanisms of NEAT1 in EC have not been fully clarified. We conducted this ... More
Emodin Attenuates Severe Acute Pancreatitis via Antioxidant and Anti-inflammatory Activity.
AuthorsXia S,Ni Y,Zhou Q,Liu H,Xiang H,Sui H,Shang D
JournalInflammation
PubMed ID31605249
Evaluation of a Novel Multiplex High-Definition PCR Assay for Detection of Tick-Borne Pathogens in Whole-Blood Specimens.
AuthorsBuchan BW,Jobe DA,Mashock M,Gerstbrein D,Faron ML,Ledeboer NA,Callister SM
JournalJournal of clinical microbiology
PubMed ID31484700
The prevalence of tick-borne infections has been steadily increasing in both number and geographic distribution in the United States and abroad. This increase, in conjunction with the continued recognition of novel pathogens transmitted by ticks, has made accurate diagnosis of these infections challenging. Mainstay serologic tests are insensitive during the ... More
S-Like-Phase Cyclin-Dependent Kinases Stabilize the Epstein-Barr Virus BDLF4 Protein To Temporally Control Late Gene Transcription.
Temporally controlled gene expression is necessary for the propagation of herpesviruses. To achieve this, herpesviruses encode several transcriptional regulators. In Epstein-Barr virus, BcRF1 associates with five viral proteins (BDLF4, BGLF3, BFRF2, BVLF1, and BDLF3.5) to form the viral late (L) gene regulatory complex, which is called the viral preinitiation complex ... More
Geranylgeraniol and Neurological Impairment: Involvement of Apoptosis and Mitochondrial Morphology.
AuthorsMarcuzzi A,Piscianz E,Zweyer M,Bortul R,Loganes C,Girardelli M,Baj G,Monasta L,Celeghini C
JournalInternational journal of molecular sciences
PubMed ID26978350
Deregulation of the cholesterol pathway is an anomaly observed in human diseases, many of which have in common neurological involvement and unknown pathogenesis. In this study we have used Mevalonate Kinase Deficiency (MKD) as a disease-model in order to investigate the link between the deregulation of the mevalonate pathway and ... More
Treatment of Coronavirus Disease 2019 (COVID-19) Patients with Convalescent Plasma.
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2, has spread globally, and no proven treatments are available. Convalescent plasma therapy has been used with varying degrees of success to treat severe microbial infections for >100 years. Patients (n = 25) with severe and/or life-threatening COVID-19 disease were enrolled ... More
Strengthening laboratory capacity for detection of respiratory viral pathogens through the Global Health Security Agenda (GHSA) framework.
AuthorsWhitaker B,Alroy KA,Guthrie E,Schildecker S,Hiers S,Woodard J,Balajee SA
JournalAfrican journal of laboratory medicine
PubMed ID31392168
BACKGROUND: Endemic and emerging respiratory viruses are a threat to public health, and a robust public health laboratory system is essential to ensure global health security. OBJECTIVE: This program sought to expand molecular laboratory testing capacity to detect a broad range of respiratory pathogens in clinical respiratory specimens collected during ... More
Lateral Flow Immunoassays for Ebola Virus Disease Detection in Liberia.
Background.âLateral flow immunoassays (LFIs) are point-of-care diagnostic assays that are designed for single use outside a formal laboratory, with in-home pregnancy tests the best-known example of these tests. Although the LFI has some limitations over more-complex immunoassay procedures, such as reduced sensitivity and the potential for false-positive results when using ... More
Two Distinct Yersinia pestis Populations Causing Plague among Humans in the West Nile Region of Uganda.
BACKGROUND: Plague is a life-threatening disease caused by the bacterium, Yersinia pestis. Since the 1990s, Africa has accounted for the majority of reported human cases. In Uganda, plague cases occur in the West Nile region, near the border with Democratic Republic of Congo. Despite the ongoing risk of contracting plague ... More
Sonic hedgehog stimulates glycolysis and proliferation of breast cancer cells: Modulation of PFKFB3 activation.
AuthorsGe X,Lyu P,Gu Y,Li L,Li J,Wang Y,Zhang L,Fu C,Cao Z
JournalBiochemical and biophysical research communications
PubMed ID26171876
Ebola Virus Infections in Nonhuman Primates Are Temporally Influenced by Glycoprotein Poly-U Editing Site Populations in the Exposure Material.
Recent experimentation with the variants of the Ebola virus that differ in the glycoproteinâs poly-uridine site, which dictates the form of glycoprotein produced through a transcriptional stutter, has resulted in questions regarding the pathogenicity and lethality of the stocks used to develop products currently undergoing human clinical trials to combat ... More
Evaluation of a Real-Time Reverse Transcription-PCR Assay for Detection of Enterovirus D68 in Clinical Samples from an Outbreak in New York State in 2014.
AuthorsZhuge J,Vail E,Bush JL,Singelakis L,Huang W,Nolan SM,Haas JP,Engel H,Della Posta M,Yoon EC,Fallon JT,Wang G
JournalJournal of clinical microbiology
PubMed ID25854481
An outbreak of severe respiratory illness associated with enterovirus D68 (EV-D68) infection was reported in mid-August 2014 in the United States. In this study, we evaluated the diagnostic utility of an EV-D68-specific real-time reverse transcription-PCR (rRT-PCR) that was recently developed by the Centers for Disease Control and Prevention in clinical ... More
KRAS mutation analysis by PCR: a comparison of two methods.
AuthorsBolton L,Reiman A,Lucas K,Timms J,Cree IA
JournalPloS one
PubMed ID25568935
BACKGROUND: KRAS mutation assays are important companion diagnostic tests to guide anti-EGFR antibody treatment of metastatic colorectal cancer. Direct comparison of newer diagnostic methods with existing methods is an important part of validation of any new technique. In this this study, we have compared the Therascreen (Qiagen) ARMS assay with ... More
Quantitative detection of SARS-CoV-2 RNA in nasopharyngeal samples from infected patients with mild disease.
AuthorsBustos P,Tambley C,Acevedo A,Andrade W,Leal G,Vidal D,Roldán F,Fasce R,Ramírez E
JournalJournal of medical virology
PubMed ID33368332
Management of a pet dog after exposure to a human patient with Ebola virus disease.
JournalJournal of the American Veterinary Medical Association
PubMed ID26295560
Anti-liver fibrosis effect of total flavonoids from Scabiosa comosa Fisch. ex Roem. et Schult. on liver fibrosis in rat models and its proteomics analysis.
Detection and serotyping of pneumococci in community acquired pneumonia patients without culture using blood and urine samples.
AuthorsElberse K,van Mens S,Cremers AJ,Meijvis SC,Vlaminckx B,de Jonge MI,Meis JF,Blauwendraat C,van de Pol I,Schouls LM
JournalBMC infectious diseases
PubMed ID25885896
BACKGROUND: Treatment of community acquired pneumonia (CAP) patients with antibiotics before laboratory-confirmed diagnosis leads to loss of knowledge on the causative bacterial pathogen. Therefore, an increasing number of pneumococcal infections is identified using non-culture based techniques. However, methods for serotyping directly on the clinical specimen remain scarce. Here we present ... More
Relationship between physical and mental health comorbidities and COVID-19 positivity, hospitalization, and mortality.
AuthorsEgede J,Campbell JA,Walker RJ,Garacci E,Dawson AZ,Egede LE
JournalJournal of affective disorders
PubMed ID33530015
BACKGROUND: Understanding the association between separate and combined mental and physical health diagnoses and COVID-19 outcomes is greatly needed to address the severity of illness. METHODS: Data on 24,034 patients screened for COVID-19 as of July 2020 were extracted from the Froedtert/Medical College of Wisconsin Epic medical record. COVID-19 outcomes ... More
Profiling and initial validation of urinary microRNAs as biomarkers in IgA nephropathy.
AuthorsWang N,Bu R,Duan Z,Zhang X,Chen P,Li Z,Wu J,Cai G,Chen X
JournalPeerJ
PubMed ID26056621
Background. MicroRNAs (miRNAs) have been found in virtually all body fluids and used successfully as biomarkers for various diseases. Evidence indicates that miRNAs have important roles in IgA nephropathy (IgAN), a major cause of renal failure. In this study, we looked for differentially expressed miRNAs in IgAN and further evaluated ... More
circTP63 functions as a ceRNA to promote lung squamous cell carcinoma progression by upregulating FOXM1.
Circular RNAs (circRNAs) are identified as vital regulators in a variety of cancers. However, the role of circRNA in lung squamous cell carcinoma (LUSC) remains largely unknown. Herein, we explore the expression profiles of circRNA and mRNA in 5 paired samples of LUSC. By analyzing the co-expression network of differentially ... More
Validation of a rapid one-step high sensitivity real-time quantitative PCR system for detecting major BCR-ABL1 mRNA on an International Scale.
AuthorsYoshida C,Nakamae H,Fletcher L,Koga D,Sogabe T,Matsumura I,Kanakura Y,Branford S,Naoe T
JournalSpringerPlus
PubMed ID27247866
BACKGROUND: Detection and quantitation of BCR-ABL1 transcripts are crucial for managing patients with chronic myeloid leukemia (CML). Although real-time quantitative polymerase chain reaction (RT-qPCR) can be measured on an International Scale (IS), this has not become fully universal. By using a WHO international standard panel established for calibrating secondary standards ... More
Cross-platform evaluation of commercial real-time reverse transcription PCR master mix kits using a quantitative 5'nuclease assay for Ebola virus.
AuthorsStephens KW,Hutchins RJ,Dauphin LA
JournalMolecular and cellular probes
PubMed ID20732412
Evaluation of nucleic acid stabilization products for ambient temperature shipping and storage of viral RNA and antibody in a dried whole blood format.
JournalThe American journal of tropical medicine and hygiene
PubMed ID25940193
Loss of sample integrity during specimen transport can lead to false-negative diagnostic results. In an effort to improve upon the status quo, we used dengue as a model RNA virus to evaluate the stabilization of RNA and antibodies in three commercially available sample stabilization products: Whatman FTA Micro Cards (GE ... More
Detection of SARS-CoV-2 Virus on Stainless-Steel Surfaces: AOAC Performance Tested MethodSM 022102.
AuthorsMehl M,Meinhardt P,Lorenzen E,Knoll C,Howaldt P,Geister J,Mergemeier S,Lacorn M
JournalJournal of AOAC International
PubMed ID34416762
BACKGROUND: The SureFast(®) SARS-CoV-2 PLUS Test is a reverse transcription qPCR (RT-qPCR) assay for the direct, qualitative detection of novel coronavirus (SARS-CoV-2) RNA from stainless-steel environmental sample swabs. OBJECTIVE: To validate the SureFast SARS-CoV-2 PLUS Kit as part of the AOAC Research Instituteâs Emergency Response Validation Performance Tested Method(s)(SM) program. ... More
Interleukin-17 alleviates erastin-induced alveolar bone loss by suppressing ferroptosis via interaction between NRF2 and p-STAT3.
AuthorsBao J,Wang Z,Yang Y,Yu X,Yuan W,Sun W,Chen L
JournalJournal of clinical periodontology
PubMed ID37961757
Validation of the COVID-19 Indoor Test™ by Phylagen for Detection of SARS-CoV-2 Virus on Stainless-Steel Surfaces: AOAC Performance Tested MethodSM 122004.
BACKGROUND: The COVID-19 Indoor Test⢠by Phylagen uses a real-time PCR Assay to detect nucleic acid from SARS-CoV-2, the causative agent of COVID-19, which is extracted from swabs sampled from environmental surfaces. This information can be used to detect the presence of the virus in indoor environments. OBJECTIVE: To validate ... More
US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was identified as the etiologic agent associated with coronavirus disease, which emerged in late 2019. In response, we developed a diagnostic panel consisting of 3 real-time reverse transcription PCR assays targeting the nucleocapsid gene and evaluated use of these assays for detecting SARS-CoV-2 ... More
Methylated SEPT9 combined with AFP and PIVKA-II is effective for the detection of HCC in high-risk population.
BACKGROUND: The methylation SEPT9 (mSEPT9) appeared to be effective for hepatocellular carcinoma (HCC) detection. However, its performance in high-risk population has not been validated. We designed a pilot study and aimed to investigate the performance of mSEPT9, AFP, PIVKA-II and their combination in hepatic cirrhosis (HC) population. METHODS: A training ... More
Racial Disparities in Incidence and Outcomes Among Patients With COVID-19.
IMPORTANCE: Initial public health data show that Black race may be a risk factor for worse outcomes of coronavirus disease 2019 (COVID-19). OBJECTIVE: To characterize the association of race with incidence and outcomes of COVID-19, while controlling for age, sex, socioeconomic status, and comorbidities. DESIGN, SETTING, AND PARTICIPANTS: This cross-sectional ... More
CNP blocks mitochondrial depolarization and inhibits SARS-CoV-2 replication in vitro and in vivo.
AuthorsLogue J,Melville VM,Ardanuy J,Frieman MB
JournalPLoS pathogens
PubMed ID38117830
The COVID-19 pandemic has claimed over 6.5 million lives worldwide and continues to have lasting impacts on the worldâs healthcare and economic systems. Several approved and emergency authorized therapeutics that inhibit early stages of the virus replication cycle have been developed however, effective late-stage therapeutical targets have yet to be ... More
Correction of Hair Shaft Defects through Allele-Specific Silencing of Mutant Krt75.
Dominant mutations in keratin genes can cause a number of inheritable skin disorders characterized by intraepidermal blistering, epidermal hyperkeratosis, or abnormalities in skin appendages, such as nail plate dystrophy and structural defects in hair. Allele-specific silencing of mutant keratins through RNA interference is a promising therapeutic approach for suppressing the ... More
Human polyclonal immunoglobulin G from transchromosomic bovines inhibits MERS-CoV in vivo.
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID26124093
Traditional approaches to antimicrobial drug development are poorly suited to combatting the emergence of novel pathogens. Additionally, the lack of small animal models for these infections hinders the in vivo testing of potential therapeutics. Here we demonstrate the use of the VelocImmune technology (a mouse that expresses human antibody-variable heavy ... More
Molecular response to imatinib & its correlation with mRNA expression levels of imatinib influx & efflux transporters in patients with chronic myeloid leukaemia in chronic phase.
AuthorsMalhotra H,Sharma P,Malhotra B,Bhargava S,Jasuja S,Kumar M
JournalThe Indian journal of medical research
PubMed ID26354214
BACKGROUND & OBJECTIVES: Imatinib is the standard first-line treatment for chronic myeloid leukaemia (CML) patients. About 20 to 30 per cent patients develop resistance to imatinib and fail imatinib treatment. One of the mechanisms proposed is varying expression levels of the drug transporters. This study was aimed to determine the ... More
Detection of the BLV provirus from nasal secretion and saliva samples using BLV-CoCoMo-qPCR-2: Comparison with blood samples from the same cattle.
Highly keratinized oral squamous cell carcinoma (OSCC) exhibits an improved response to treatment and prognosis compared with weakly keratinized OSCC. Therefore, we aimed to develop gene transcript signature and to identify novel full-length isoforms, fusion transcript and non-coding RNA to differentiate well-differentiated (WD) with Moderately Differentiated (MD)/Poorly Differentiated (PD)/WD-lymphadenopathy OSCC ... More
Effect of Developmental Stages on Genes Involved in Middle and Downstream Pathway of Volatile Terpene Biosynthesis in Rose Petals.
AuthorsKong Y,Wang H,Lang L,Dou X,Bai J
JournalGenes
PubMed ID35885960
Terpenoids are economically and ecologically important compounds, and they are vital constituents in rose flower fragrance and rose essential oil. The terpene synthase genes (TPSs), trans-prenyltransferases genes (TPTs), NUDX1 are involved in middle and downstream pathway of volatile terpene biosynthesis in rose flowers. We identified 7 complete RcTPTs, 49 complete ... More
Identification of SRXN1 and KRT6A as Key Genes in Smoking-Related Non-Small-Cell Lung Cancer Through Bioinformatics and Functional Analyses.
AuthorsZhou J,Jiang G,Xu E,Zhou J,Liu L,Yang Q
JournalFrontiers in oncology
PubMed ID35071014
BACKGROUND: Lung cancer is the leading cause of cancer-related mortality worldwide. Although cigarette smoking is an established risk factor for lung cancer, few reliable smoking-related biomarkers for non-small-cell lung cancer (NSCLC) are available. An improved understanding of these biomarkers would further the development of new biomarker-targeted therapies and lead to ... More