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Invitrogen
Phospho-ETS1 (Ser282, Ser285) Polyclonal Antibody
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FIGURE: 1 / 7
Phospho-ETS1 (Ser282, Ser285) Antibody (44-1111G) in ICC/IF
Immunofluorescence analysis of ETS (pS282/pS285) was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ETS (pS282/pS285) Rabbit Polyclonal Antibody (Product # 44-1111G) at 1:250 dilution in 1% BSA and incubated for ... View More
Product Details
44-1111G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ETS1 that contains both serine 282 and 285.
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homlog 1 (avian); ETS-1; EWSR2; p54), a 441 A. A protein, is a member of DNA-binding ETS protein family. It is a multifunctional protein consisting of an N-terminal pointed domain, a central transactivation domain and 2 auto inhibitory domains flanked on both sides of conserved C-terminal Ets domain. It interacts with factors including PEBP2alphaA, p300/CBP and mediates transactivation of Osteopontin, Presenilin-1, Stromelysin and Collagenase. It is activated by Ras/MAPK signaling and its expression is up-regulated in resting T-cells. Its activity is modulated by various factors including AP-1, Sp-1, c-myb and MafB. Sp100 activates ETS1 in nuclear bodies. It interacts with EAP1/Daxx and causes repression of MMP1 and BCL2 transactivation. It interacts with STAT6 and modulates Socs-1 expression induced by IL-4. It interacts with GATA3 and positively regulates Tax-1 dependent IL-5 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1; Ets avian erythroblastosis virus E2 oncogene homolog 1 (tumor progression locus 1); ets protein; ets-1 protein; Ets-1Delta(III-VI); ETS1; FLJ10768; p54; Protein C-ets-1; unnamed protein product; v-ets avian erythroblastosis virus E2 oncogene homolog 1; v-ets avian erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1
Gene Aliases: c-ets-1; ETS-1; ETS1; Etsoncb; EWSR2; p54; Tpl1
UniProt ID: (Human) P14921, (Rat) P41156
Entrez Gene ID: (Human) 2113, (Rat) 24356
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription corepressor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
histone acetyltransferase binding
winged helix/forkhead transcription factor
immune system process
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
immune response
negative regulation of cell proliferation
positive regulation of endothelial cell migration
positive regulation of gene expression
cell differentiation
PML body organization
regulation of apoptotic process
positive regulation of blood vessel endothelial cell migration
positive regulation of erythrocyte differentiation
regulation of angiogenesis
positive regulation of angiogenesis
negative regulation of cell cycle
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to antibiotic
cell motility
positive regulation of inflammatory response
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of leukocyte adhesion to vascular endothelial cell
response to hypoxia
female pregnancy
positive regulation of cell proliferation
response to wounding
response to mechanical stimulus
regulation of extracellular matrix disassembly
hypothalamus development
pituitary gland development
positive regulation of cell migration
response to estradiol
response to laminar fluid shear stress
estrous cycle
positive regulation of cellular component movement
angiogenesis involved in wound healing
cellular response to hydrogen peroxide
response to interleukin-1
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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