Applied Biosystems™

TaqMan™ Universal Master Mix II, with UNG

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

TaqMan® Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). You can use TaqMan® Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan® Universal Master Mix II is supplied as a 2X concentrate and contains:

• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX™ Passive Reference
• Uracil-N glycosylase (UNG)
• Optimized buffer components

Note: This TaqMan® Universal Master Mix II contains Uracil-N glycoslyase (UNG). See a complete listing of all TaqMan® Universal Master Mix II products, with and without UNG here.

TaqMan® Universal Master Mix II has been optimized for use with primers and TaqMan® probes that have been designed according to Life Technologies' guidelines. The master mix can be used with custom TaqMan® assays available from our custom assay service, or with pre-optimized assays such as:

• TaqMan® Gene Expression Assays
• TaqMan® MicroRNA Assays
• TaqMan® Drug Metabolism Genotyping Assays
• TaqMan® SNP Genotyping Assays

For RNA quantitation experiments, the TaqMan® Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Life Technologies.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7300 System, 7500 System, 7900HT System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: TaqMan™
Product Size: 1 x 5 mL
Purity or Quality Grade: UP (Ultra Pure)
Sample Type (Specific): DNA (Genomic), cDNA
Shipping Condition: Wet Ice

Contents & storage

Store at 4°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols