Applied Biosystems™

TaqMan™ Universal Master Mix II, no UNG

Catalog number: 4440047
Applied Biosystems™

TaqMan™ Universal Master Mix II, no UNG

Catalog number: 4440047
Catalog Number
4440047
Unit Size
2 x 5 mL
Price (USD)
Price: 988.00
Your Price:
Availability
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Quantity
Catalog Number
Unit Size
1 x 1 mL
Price (USD)
Price: 113.00
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Availability
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Quantity
Catalog Number
Unit Size
1 x 5 mL
Price (USD)
Price: 520.00
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Quantity
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Unit Size
5 x 5 mL
Price (USD)
Price: 2,415.00
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Quantity
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Unit Size
10 x 5 mL
Price (USD)
Price: 4,575.00
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Quantity
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Unit Size
1 x 50 mL
Price (USD)
Price: 4,590.00
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
44400472 x 5 mL
Price: 988.00
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44400431 x 1 mL
Price: 113.00
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44400401 x 5 mL
Price: 520.00
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44400485 x 5 mL
Price: 2,415.00
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444004910 x 5 mL
Price: 4,575.00
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44400411 x 50 mL
Price: 4,590.00
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Product Overview
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Additional Information
Recommendations
Recommendations
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Optimized buffer components

Note: This TaqMan Universal Master Mix II does not contain uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service or with pre-optimized assays, such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping Assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Real Time PCR (qPCR)
Concentration
2X
Detection Method
Primer-Probe
Fidelity (vs. Taq)
2 X
GC-Rich PCR Performance
High
PCR Method
qPCR
Polymerase
AmpliTaq Gold DNA Polymerase
Reaction Speed
Standard
Sample Type
DNA (Genomic), cDNA
Volume
10 mL
For Use With (Equipment)
7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Passive Reference Dye
ROX (Pre-mixed)
Primer Probe Compatibility
TaqMan™
Product Line
TaqMan™
Product Type
Universal Master Mix II
Purity or Quality Grade
UP (Ultra Pure)
Quantity
2 x 5 mL

Contents & Storage

Store at 4°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

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