Applied Biosystems™

TaqMan™ Universal Master Mix II, no UNG

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Description

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Optimized buffer components

Note: This TaqMan Universal Master Mix II does not contain uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service or with pre-optimized assays, such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping Assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
For Use With (Equipment): 7300 System, 7500 System, 7900HT System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: TaqMan™
Product Size: 2 x 5 mL
Purity or Quality Grade: UP (Ultra Pure)
Sample Type (Specific): DNA (Genomic), cDNA

Contents & storage

Store at 4°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Documents

Manuals & protocols