Veriti™ Dx 96-well Thermal Cycler, 0.2 mL - FAQs

View additional product information for Veriti™ Dx 96-well Thermal Cycler, 0.2 mL - FAQs (4452300)

2 product FAQs found

Which thermal cyclers have been tested for use with Cytoscan HD?

We recommend using the following thermal cyclers with Cytoscan HD:

For pre-PCR, we recommend 2720 Thermal Cycler (Cat. No. 4359659)

For pre- and post-PCR , we recommend the following thermal cyclers:
- GeneAmp PCR System 9700 Gold (Cat. No. 4314878) (Note: This instrument has been discontinued and therefore cannot be ordered)
- GeneAmp PCR System 9700 Silver (Cat. No. N8050001) (Note: This instrument has been discontinued and therefore cannot be ordered)
- Applied Biosystems Veriti Dx 96-Well Thermal Cycler (Cat. No. 4452300)
- Applied Biosystems Veriti 96-Well Thermal Cycler (Cat. No. 4375786)
- Bio-Rad® DNA Engine® PTC-200 Thermal Cycler
- Eppendorf® Mastercycler® Pro S Thermal Cycler

Note: You must use thermal cyclers with silver or gold-plated silver blocks. Do not use thermal cyclers with aluminum blocks.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.