MagMAX™ Pathogen RNA/DNA Kit

Catalog number: 4462359

Applied Biosystems™  Related applications: Animal Health Sample Preparation

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Description

The Applied Biosystems® MagMAX™ Pathogen RNA/DNA Kit enables purification of RNA and DNA from viruses and easy-to-lyse bacteria and parasites, using magnetic particle technology in a 96-well format. PCR inhibitors are effectively removed in the process, making the resulting nucleic acid ideal for real-time PCR and RT-PCR applications.

Key Features:

• Convenience and flexibility: one kit for RNA and DNA, suitable for a wide range of sample types sample input volumes
• Increased confidence in your results: fewer false-negatives due to effective PCR inhibitor removal
• Improved workflow efficiency: process 96 samples in approximately 45 minutes using the MagMAX™ Express-96 Deep Well Magnetic Particle Processor

The MagMAX™ Pathogen RNA/DNA Kit has been optimized to provide a convenient, flexible solution (Fig. 1). This single kit can accomodate:

Purification of RNA and DNA
• Purification from viruses and easy-to-lyse bacteria and parasites
• Low and high sample input volumes
• A wide range of sample types:
• Low cell content samples (e.g., serum and plasma; nasal, tracheal, and cloacal swabs; ear notches)
• Whole blood
• Semen
• Oral fluid
• Feces
• Biomed Diagnostics InPouch™ TF (Tritrichomonas foetus) culture

The MagMAX™ Pathogen RNA/DNA Kit is Easy to Automate
Purify RNA/DNA from 96 samples in approximately 45 minutes (Fig. 2) using the MagMAX™ Express-96 Deep Well Magnetic Particle Processor. Low and high sample input volumes can be used for most sample types, and multiple sample types can be processed at the same time on the same plate after sample-specific pre-processing, saving time and plastics.

Increase the Accuracy of Your Results
Reduce false-negative results due to the effective PCR inhibitor removal achieved by the MagMAX™ Pathogen RNA/DNA Kit. Magnetic particles bind RNA/DNA more efficiently and are more thoroughly washed than glass fiber filters, resulting in higher yields and purity of RNA/DNA. In addition, no sample cross contamination has been observed due to extensive optimization of the MagMAX™ Express-96 Deep Well Magnetic Particle Processor programs.
For Veterinary Use Only.

Specifications

Sample Type (General): Liquid Samples (e.g. Serum), Other Samples, Viral Samples, Blood, Bacteria
High Throughput Compatibility: Automated Protocols, Not High Throughput-Compatible (Manual), High Throughput-Compatible
Downstream Application: Reverse Transcriptase PCR (RT-PCR), PCR, Real-Time Quantitative PCR (qPCR)
Format: 96-well plate
Product Size: 480 preps
Final Product: Viral DNA⁄RNA, Pathogen DNA⁄RNA
Purification Time: 45 min
Number of Reactions: 480 Preps
Isolation Technology: Magnetic Bead
Sample Type (Specific): Plasma, Parasite Samples, BioMed Diagnostics InPouch™ TF Culture, Ear Notches, Mosquitoe, Viral Sample, Viral Transport Media Samples, Any Swab Type, Whole Blood, Cell-Free Fluids, Feces, Bacteria, Saliva, Tissue Homogenates, Serum
For Use With (Equipment): MagMAX™ Express-96 Deep Well Magnetic Particle Processor, KingFisher™ Flex Magnetic Particle Processor, MagMAX™ Express Magnetic Particle Processor, MagMAX™ Express-96 Standard Magnetic Particle Processor

Contents & storage

Store at room temperature:
1 bottle of Lysis⁄Binding Solution Concentrate
1 bottle of Wash Solution 1 Concentrate
2 bottles of Wash Solution 2 Concentrate
1 bottle of Elution Buffer

Store at 4 °C:
1 bottle of Nucleic Acid Binding Beads

Store at -20 °C:
1 bottle of Lysis ENHANCER
5 tubes of Carrier Nucleic Acid

Documents

Manuals & protocols

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