miR-185-5p Regulates Inflammation and Phagocytosis through CDC42/JNK Pathway in Macrophages.
AuthorsMa X,Liu H,Zhu J,Zhang C,Peng Y,Mao Z,Jing Y,Chen F
JournalGenes
PubMed ID35328023
Macrophage activation is an essential component of systemic chronic inflammation and chronic inflammatory diseases. Emerging evidence implicates miR-185-5p in chronic inflammation diseases. However, the regulatory role of miR-185-5p in macrophage pro-inflammatory activation has not been studied previously. Here, we identified that miR-185-5p was one of the top genes and effectively ... More
Fetal Therapy Model of Myelomeningocele with Three-Dimensional Skin Using Amniotic Fluid Cell-Derived Induced Pluripotent Stem Cells.
Myelomeningocele (MMC) is a congenital disease without genetic abnormalities. Neurological symptoms are irreversibly impaired after birth, and no effective treatment has been reported to date. Only surgical repairs have been reported so far. In this study, we performed antenatal treatment of MMC with an artificial skin using induced pluripotent stem ... More
Turmeric Bioactive Compounds Alleviate Spinal Nerve Ligation-Induced Neuropathic Pain by Suppressing Glial Activation and Improving Mitochondrial Function in Spinal Cord and Amygdala.
This study examined the effects of turmeric bioactive compounds, curcumin C3 complex® (CUR) and bisdemethoxycurcumin (BDMC), on mechanical hypersensitivity and the gene expression of markers for glial activation, mitochondrial function, and oxidative stress in the spinal cord and amygdala of rats with neuropathic pain (NP). Twenty-four animals were randomly assigned ... More
Association between SNPs in microRNAs and microRNAs-Machinery Genes with Susceptibility of Leprosy in the Amazon Population.
JournalInternational journal of molecular sciences
PubMed ID36142557
Leprosy is a chronic neurodermatological disease caused by the bacillus Mycobacterium leprae. Recent studies show that SNPs in genes related to miRNAs have been associated with several diseases in different populations. This study aimed to evaluate the association of twenty-five SNPs in genes encoding miRNAs related to biological processes and ... More
Toll-Like Receptors 2, 4, and 7, Interferon-Gamma, Interleukin 10, and Programmed Death Ligand 1 Transcripts in Leishmanin Skin Test-Positive Reactions of Ibizan Hound Dogs.
AuthorsOrdeix L,Montserrat-Sangrà S,Martínez-Orellana P,Solano-Gallego L
JournalJournal of immunology research
PubMed ID32211445
The leishmanin skin test (LST) is an in vivo technique commonly used to evaluate the Leishmania-specific cellular immune response in dogs. However, information regarding the local immune response in LST-positive reactions is scarce. We examined the pattern of toll-like receptor 2 (TLR2), TLR4, TLR7, interleukin- (IL-) 10, interferon gamma (IFN-γ), ... More
Preclinical immunogenicity and efficacy of a candidate COVID-19 vaccine based on a vesicular stomatitis virus-SARS-CoV-2 chimera.
BACKGROUND: To investigate a vaccine technology with potential to protect against coronavirus disease 2019 (COVID-19) and reduce transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with a single vaccine dose, we developed a SARS-CoV-2 candidate vaccine using the live vesicular stomatitis virus (VSV) chimeric virus approach previously used to ... More
The miR-124-AMPAR pathway connects polygenic risks with behavioral changes shared between schizophrenia and bipolar disorder.
Schizophrenia (SZ) and bipolar disorder (BP) are highly heritable major psychiatric disorders that share a substantial portion of genetic risk as well as their clinical manifestations. This raises a fundamental question of whether, and how, common neurobiological pathways translate their shared polygenic risks into shared clinical manifestations. The present study ... More
Comparison of MicroRNA Expression in Aqueous Humor of Normal and Primary Open-Angle Glaucoma Patients Using PCR Arrays: A Pilot Study.
PURPOSE: MicroRNAs (miRNAs) are small, endogenous noncoding RNAs that have been detected in human aqueous humor (AH). Prior studies have pooled samples to obtain sufficient quantities for analysis or used next-generation sequencing. Here, we used PCR arrays with preamplification to identify and compare miRNAs from individual AH samples between patients ... More
Striatal Neurons Partially Expressing a Dopaminergic Phenotype: Functional Significance and Regulation.
AuthorsTroshev D,Bannikova A,Blokhin V,Kolacheva A,Pronina T,Ugrumov M
JournalInternational journal of molecular sciences
PubMed ID36232359
Since the discovery of striatal neurons expressing dopamine-synthesizing enzymes, researchers have attempted to identify their phenotype and functional significance. In this study, it was shown that in transgenic mice expressing green fluorescent protein (GFP) under the tyrosine hydroxylase (TH) gene promoter, (i) there are striatal neurons expressing only TH, only ... More
The retrograde signaling regulator ANAC017 recruits the MKK9-MPK3/6, ethylene, and auxin signaling pathways to balance mitochondrial dysfunction with growth.
AuthorsHe C,Liew LC,Yin L,Lewsey MG,Whelan J,Berkowitz O
JournalThe Plant cell
PubMed ID35708648
In plant cells, mitochondria are ideally positioned to sense and balance changes in energy metabolism in response to changing environmental conditions. Retrograde signaling from mitochondria to the nucleus is crucial for adjusting the required transcriptional responses. We show that ANAC017, the master regulator of mitochondrial stress, directly recruits a signaling ... More
TRF1 uses a noncanonical function of TFIIH to promote telomere replication.
AuthorsYang Z,Sharma K,de Lange T
JournalGenes & development
PubMed ID36229075
Telomeric DNA challenges the replisome and requires TRF1 for efficient duplication. TRF1 recruits the BLM helicase, but BLM loss does not explain the extensive telomere fragility, ATR signaling, and sister telomere associations (STAs) induced by TRF1 deletion. Here, we document that Helix2 of the TRFH domain and Helix1 of the ... More
Ceramides are necessary and sufficient for diet-induced impairment of thermogenic adipocytes.
AuthorsChaurasia B,Ying L,Talbot CL,Maschek JA,Cox J,Schuchman EH,Hirabayashi Y,Holland WL,Summers SA
JournalMolecular metabolism
PubMed ID33352310
OBJECTIVE: Aging and weight gain lead to a decline in brown and beige adipocyte functionality that exacerbates obesity and insulin resistance. We sought to determine whether sphingolipids, such as ceramides, a class of lipid metabolites that accumulate in aging and overnutrition, are sufficient or necessary for the metabolic impairment of ... More
Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model.
The developmental origins of health and disease concept links adult diseases with early-life exposure to inappropriate environmental conditions. Intrauterine and postnatal malnutrition may lead to an increased incidence of type 2 diabetes, obesity, and cardiovascular diseases. Maternal malnutrition (MM) has also been associated with prostate carcinogenesis. However, the molecular mechanisms ... More
Tracheal Aspirate as an Alternative Biologic Sample for Pharmacogenomics Testing in Mechanically Ventilated Pediatric Patients.
AuthorsHargreaves KA,Pratt VM,Medeiros EB,Lynnes TC,Granfield CA,Skaar TC,Iwata-Otsubo A,Tillman EM
JournalClinical and translational science
PubMed ID32702149
Patients in the pediatric intensive care unit are exposed to multiple medications and are at high risk for adverse drug reactions. Pharmacogenomic (PGx) testing could help decrease their risk of adverse reactions. Although whole blood is preferred for PGx testing, blood volume in this population is often limited. However, for ... More
Implication of miR-126 and miR-139-5p in Plasmacytoid Dendritic Cell Dysregulation in Systemic Sclerosis.
Compelling evidence shows the involvement of plasmacytoid dendritic cells (pDCs) in systemic sclerosis (SSc) pathogenesis. This study investigated whether microRNAs (miRNAs) are involved in the dysregulation of pDCs in SSc patients already at early stages. RNA from circulating pDCs was isolated from two independent cohorts of SSc patients with different ... More
Identification of circular RNAs in porcine sperm and evaluation of their relation to sperm motility.
AuthorsGòdia M,Castelló A,Rocco M,Cabrera B,Rodríguez-Gil JE,Balasch S,Lewis C,Sánchez A,Clop A
JournalScientific reports
PubMed ID32409652
Circular RNAs (circRNAs) are emerging as a novel class of noncoding RNAs which potential role as gene regulators is quickly gaining interest. circRNAs have been studied in different tissues and cell types across several animal species. However, a thorough characterization of the circRNAome in ejaculated sperm remains unexplored. In this ... More
Host genotype and amoxicillin administration affect the incidence of diarrhoea and faecal microbiota of weaned piglets during a natural multiresistant ETEC infection.
Tissue-scale tensional homeostasis in skin regulates structure and physiological function.
AuthorsKimura S,Tsuchiya A,Ogawa M,Ono M,Suda N,Sekimoto K,Takeo M,Tsuji T
JournalCommunications biology
PubMed ID33127987
Tensional homeostasis is crucial for organ and tissue development, including the establishment of morphological and functional properties. Skin plays essential roles in waterproofing, cushioning and protecting deeper tissues by forming internal tension-distribution patterns, which involves aligning various cells, appendages and extracellular matrices (ECMs). The balance of traction force is thought ... More
A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells.
Cigarette Smoke Induces Metabolic Reprogramming of the Tumor Stroma in Head and Neck Squamous Cell Carcinoma.
AuthorsDomingo-Vidal M,Whitaker-Menezes D,Martos-Rus C,Tassone P,Snyder CM,Tuluc M,Philp N,Curry J,Martinez-Outschoorn U
JournalMolecular cancer research : MCR
PubMed ID31239287
Head and neck squamous cell carcinoma (HNSCC) is comprised of metabolically linked distinct compartments. Cancer-associated fibroblasts (CAFs) and non-proliferative carcinoma cells display a glycolytic metabolism, whilst proliferative carcinoma cells rely on mitochondrial oxidative metabolism fueled by the catabolites provided by the adjacent CAFs. Metabolic coupling between these reprogrammed compartments contributes ... More
Response of human oral mucosal epithelial cells to different storage temperatures: A structural and transcriptional study.
PURPOSE: Seeking to improve the access to regenerative medicine, this study investigated the structural and transcriptional effects of storage temperature on human oral mucosal epithelial cells (OMECs). METHODS: Cells were stored at four different temperatures (4°C, 12°C, 24°C and 37°C) for two weeks. Then, the morphology, cell viability and differential ... More
Cystic Fibrosis Polymorphic Variants in a Russian Population.
PURPOSE: Cystic fibrosis (CF) is one of the most common monogenic diseases with an autosomal recessive inheritance. Carrier screening leads to a reduction in the number of children born with CF disease. The aim of this study was to develop the custom panel for the diagnosis of heterozygous carriage of ... More
Cadmium-Induced Renal Cell Toxicity Is Associated With MicroRNA Deregulation.
AuthorsLemaire J,Van der Hauwaert C,Savary G,Dewaeles E,Perrais M,Lo Guidice JM,Pottier N,Glowacki F,Cauffiez C
JournalInternational journal of toxicology
PubMed ID31934807
The strength and form of natural selection on transcript abundance in the wild.
AuthorsAhmad F,Debes PV,Nousiainen I,Kahar S,Pukk L,Gross R,Ozerov M,Vasemägi A
JournalMolecular ecology
PubMed ID33219570
Gene transcription variation is known to contribute to disease susceptibility and adaptation, but we currently know very little about how contemporary natural selection shapes transcript abundance. Here, we propose a novel analytical framework to quantify the strength and form of ongoing natural selection at the transcriptome level in a wild ... More
Functional lipidomics of vascular endothelial cells in response to laminar shear stress.
AuthorsHirata T,Yamamoto K,Ikeda K,Arita M
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
PubMed ID33421194
Social Experience Regulates Endocannabinoids Modulation of Zebrafish Motor Behaviors.
AuthorsOrr SA,Ahn S,Park C,Miller TH,Kassai M,Issa FA
JournalFrontiers in behavioral neuroscience
PubMed ID34045945
Social status-dependent modulation of neural circuits has been investigated extensively in vertebrate and invertebrate systems. However, the effects of social status on neuromodulatory systems that drive motor activity are poorly understood. Zebrafish form a stable social relationship that consists of socially dominant and subordinate animals. The locomotor behavior patterns differ ... More
Biotechnical development of genetic addiction risk score (GARS) and selective evidence for inclusion of polymorphic allelic risk in substance use disorder (SUD).
AuthorsBlum K,Bowirrat A,Baron D,Lott L,Ponce JV,Brewer R,Siwicki D,Boyett B,Gondre-Lewis MC,Smith DE,Panayotis K T,Badgaiyan S,Hauser M,Fried L,A R 3rd,Downs BW,Badgaiyan RD
JournalJournal of systems and integrative neuroscience
PubMed ID33614164
Research into the neurogenetic basis of addiction identified and characterized by Reward Deficiency Syndrome (RDS) includes all drug and non-drug addictive, obsessive and compulsive behaviors. We are proposing herein that a new model for the prevention and treatment of Substance Use Disorder (SUD) a subset of RDS behaviors, based on ... More
Reduced Gonadotrophin Receptor Expression Is Associated with a More Aggressive Ovarian Cancer Phenotype.
AuthorsCheung J,Lokman NA,Abraham RD,Macpherson AM,Lee E,Grutzner F,Ghinea N,Oehler MK,Ricciardelli C
JournalInternational journal of molecular sciences
PubMed ID33374698
Follicle-stimulating hormone (FSH) and luteinising hormone (LH) play important roles in regulating cell growth and proliferation in the ovary. However, few studies have explored the expression of FSH and LH receptors (FSHR and LHCGR) in ovarian cancer, and their functional roles in cancer progression remain inconclusive. This study investigated the ... More
Pregnancy-associated changes in cervical noncoding RNA.
AIM: To identify pregnancy-associated changes in cervical noncoding RNA (ncRNA), including miRNA and long noncoding RNA (lncRNA), and their potential effects on biologic processes. MATERIALS & METHODS: We enrolled 21 pregnant women with term deliveries (â¥37 weeksâ gestation) in a prospective cohort and collected cervical swabs before 28 weeksâ gestation. We enrolled ... More
The peripheral taste and olfactory systems in mammals are separate and independent sensory systems. In the current model of chemosensation, gustatory, and olfactory receptors are genetically divergent families expressed in anatomically distinct locations that project to disparate downstream targets. Although information from the 2 sensory systems merges to form the ... More
Fatty Acid and Carnitine Metabolism Are Dysregulated in Systemic Sclerosis Patients.
AuthorsOttria A,Hoekstra AT,Zimmermann M,van der Kroef M,Vazirpanah N,Cossu M,Chouri E,Rossato M,Beretta L,Tieland RG,Wichers CGK,Stigter E,Gulersonmez C,Bonte-Mineur F,Berkers CR,Radstake TRDJ,Marut W
JournalFrontiers in immunology
PubMed ID32528464
Systemic sclerosis (SSc) is a rare chronic disease of unknown pathogenesis characterized by fibrosis of the skin and internal organs, vascular alteration, and dysregulation of the immune system. In order to better understand the immune system and its perturbations leading to diseases, the study of the mechanisms regulating cellular metabolism ... More
The MS4A gene cluster is a key modulator of soluble TREM2 and Alzheimer's disease risk.
Soluble triggering receptor expressed on myeloid cells 2 (sTREM2) in the cerebrospinal fluid (CSF) has been associated with Alzheimer disease (AD). TREM2 plays a critical role in microglial activation, survival, and phagocytosis; however, the pathophysiological role of sTREM2 in AD is not well understood. Understanding the role of sTREM2 in ... More
Interaction between catechol-O-methyltransferase polymorphism and childhood trauma in suicidal ideation of patients with post-traumatic stress disorder.
AuthorsKwon A,Min D,Kim Y,Jin MJ,Lee SH
JournalBrain and behavior
PubMed ID32618128
INTRODUCTION: Suicidal behavior of postâtraumatic stress disorder (PTSD) patients is influenced by genetic and environmental factors. The catecholâOâmethyltransferase (COMT) gene has been known to be associated with suicidal ideation. The present study aimed to explore the relationship of COMT polymorphism, childhood trauma, and suicidal ideation in patients with PTSD. METHODS: ... More
Functional analysis of miR-21-3p, miR-30b-5p and miR-150-5p shuttled by extracellular vesicles from diabetic subjects reveals their association with diabetic retinopathy.
AuthorsMazzeo A,Lopatina T,Gai C,Trento M,Porta M,Beltramo E
JournalExperimental eye research
PubMed ID31002820
Non-Coding RNAs as Prognostic Biomarkers: A miRNA Signature Specific for Aggressive Early-Stage Lung Adenocarcinomas.
Lung cancer burden can be reduced by adopting primary and secondary prevention strategies such as anti-smoking campaigns and low-dose CT screening for high risk subjects (aged >50 and smokers >30 packs/year). Recent CT screening trials demonstrated a stage-shift towards earlier stage lung cancer and reduction of mortality (~20%). However, a ... More
Transcriptome Profiling of Potato (Solanum tuberosum L.) Responses to Root-Knot Nematode (Meloidogyne javanica) Infestation during A Compatible Interaction.
AuthorsMacharia TN,Bellieny-Rabelo D,Moleleki LN
JournalMicroorganisms
PubMed ID32967109
Root-knot nematode (RKN) Meloidogyne javanica presents a great challenge to Solanaceae crops, including potato. In this study, we investigated transcriptional responses of potato roots during a compatible interaction with M. javanica. In this respect, differential gene expression of Solanum tuberosum cultivar (cv.) Mondial challenged with M. javanica at 0, 3 ... More
SARS-CoV-2 infection induces germinal center responses with robust stimulation of CD4 T follicular helper cells in rhesus macaques.
CD4 T follicular helper (T(fh)) cells are important for the generation of long-lasting and specific humoral protection against viral infections. The degree to which SARS-CoV-2 infection generates T(fh) cells and stimulates the germinal center response is an important question as we investigate vaccine options for the current pandemic. Here we ... More
Quantifying the Role of Ground Beetles for the Dispersal of Fusarium and Alternaria Fungi in Agricultural Landscapes.
The spread by arthropods (zoochory) is an essential dispersal mechanism for many microorganisms, like plant pathogens. Carabid beetles are very abundant and mobile ground-dwelling insects. However, their role in the dispersal of economically relevant phytopathogens, like Fusarium and Alternaria fungi is basically unknown. We quantified the total fungal, Fusarium, and ... More
Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death.
Interferon-inducible guanylate-binding proteins (GBPs) promote cell-intrinsic defense through host cell death. GBPs target pathogens and pathogen-containing vacuoles and promote membrane disruption for release of microbial molecules that activate inflammasomes. GBP1 mediates pyroptosis or atypical apoptosis of Salmonella Typhimurium (STm)- or Toxoplasma gondii (Tg)- infected human macrophages, respectively. The pathogen-proximal detection-mechanisms ... More
Mitochondrial function modulates touch signalling in Arabidopsis thaliana.
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID33452132
OCT4 is a fundamental component of the molecular circuitry governing pluripotency in vivo and in vitro. To determine how OCT4 establishes and protects the pluripotent lineage in the embryo, we used comparative single-cell transcriptomics and quantitative immunofluorescence on control and OCT4 null blastocyst inner cell masses at two developmental stages. ... More
MYO5B mutations in pheochromocytoma/paraganglioma promote cancer progression.
Identification of additional cancer-associated genes and secondary mutations driving the metastatic progression in pheochromocytoma and paraganglioma (PPGL) is important for subtyping, and may provide optimization of therapeutic regimens. We recently reported novel recurrent nonsynonymous mutations in the MYO5B gene in metastatic PPGL. Here, we explored the functional impact of these ... More
Spontaneous rescue of a FMR1 repeat expansion and review of deletions in the FMR1 non-coding region.
AuthorsErbs E,Fenger-Grøn J,Jacobsen CM,Lildballe DL,Rasmussen M
JournalEuropean journal of medical genetics
PubMed ID34022415
Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues.
Like all obligate intracellular pathogens, influenza A virus (IAV) reprograms host cellâs glucose and lipid metabolism to promote its own replication. However, the impact of influenza infection on white adipose tissue (WAT), a key tissue in the control of systemic energy homeostasis, has not been yet characterized. Here, we show ... More
The combination of resveratrol and exercise enhances muscle growth characteristics in pacu (Piaractus mesopotamicus).
AuthorsSalomão RAS,De Paula TG,Zanella BTT,Carvalho PLPF,da Silva Duran BO,Valente JS,de Almeida Fantinatti BE,Fernandes AA,Barros MM,Mareco EA,Carvalho RF,Dos Santos VB,Dal-Pai-Silva M
JournalComparative biochemistry and physiology. Part A, Molecular & integrative physiology
PubMed ID31077846
Urinary Extracellular Vesicles Carrying Klotho Improve the Recovery of Renal Function in an Acute Tubular Injury Model.
JournalMolecular therapy : the journal of the American Society of Gene Therapy
PubMed ID31818691
Acute kidney injury, defined by a rapid deterioration of renal function, is a common complication in hospitalized patients. Among the recent therapeutic options, the use of extracellular vesicles (EVs) is considered a promising strategy. Here we propose a possible therapeutic use of renal-derived EVs isolated from normal urine (urine-derived EVs ... More
Feasibility of a multiparametric MRI protocol for imaging biomarkers associated with neoadjuvant radiotherapy for soft tissue sarcoma.
OBJECTIVE: Soft tissue sarcoma (STS) is a rare malignancy with a 5âyear overall survival rate of 55%. Neoadjuvant radiotherapy is commonly used in preparation for surgery, but methods to assess early response are lacking despite pathological response at surgery being predictive of overall survival, local recurrence and distant metastasis. Multiparametric ... More
Detection of IgM, IgG and SARS-CoV-2 RNA among the personnel of the University of Milan, March through May 2020: the UNICORN study.
OBJECTIVES: In Italy, the pandemic of COVID-19 resulted in congestion of hospitals and laboratories and probably determined an underestimation of the number of infected subjects, as the molecular diagnosis of SARS-CoV-2 infection was mainly performed on hospitalised patients. Therefore, limited data are available about the number of asymptomatic/paucisymptomatic subjects in ... More
Effects of Different Exercise Training Protocols on Gene Expression of Rac1 and PAK1 in Healthy Rat Fast- and Slow-Type Muscles.
PURPOSE: Rac1 and its downstream target PAK1 are novel regulators of insulin and exercise-induced glucose uptake in skeletal muscle. However, it is not yet understood how different training intensities affect the expression of these proteins. Therefore, we studied the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) ... More
Scratch2, a Snail Superfamily Member, Is Regulated by miR-125b.
AuthorsGoes CP,Vieceli FM,De La Cruz SM,Simões-Costa M,Yan CYI
JournalFrontiers in cell and developmental biology
PubMed ID32984310
Scratch2 is a transcription factor expressed in a very restricted population of vertebrate embryonic neural cell precursors involved in their survival, differentiation, and migration. The mechanisms that control its expression remain unknown and could contribute towards our understanding of gene regulation during neural differentiation and evolution. Here we investigate the ... More
Capsaicin lacks tumor-promoting effects during colon carcinogenesis in a rat model induced by 1,2-dimethylhydrazine.
AuthorsCaetano BFR,Tablas MB,Ignoti MG,de Moura NA,Romualdo GR,Barbisan LF,Rodrigues MAM
JournalEnvironmental science and pollution research international
PubMed ID32886307
Impact of the Na(V)1.8 variant, A1073V, on post-sigmoidectomy pain and electrophysiological function in rat sympathetic neurons.
AuthorsCoates MD,Kim JS,Carkaci-Salli N,Vrana KE,Koltun WA,Puhl HL,Adhikary SD,Janicki PK,Ruiz-Velasco V
JournalJournal of neurophysiology
PubMed ID31642403
Na(V)1.8 channels play a crucial role in regulating the action potential in nociceptive neurons. A single nucleotide polymorphism in the human Na(V)1.8 gene SCN10A, A1073V (rs6795970, G>A), has been linked to the diminution of mechanical pain sensation as well as cardiac conduction abnormalities. Furthermore, studies have suggested that this polymorphism ... More
Promoterless Transposon Mutagenesis Drives Solid Cancers via Tumor Suppressor Inactivation.
SIMPLE SUMMARY: Over the past two decades, there have been many published studies reporting high-copy SB transgenic lines in which the transposon allele includes both gene-trap and internal promoter elements to drive tumorigenesis in vivo. Cancer gene discovery from end-stage solid tumors in these studies performed by our labs and ... More
Validation of a 40-gene expression profile test to predict metastatic risk in localized high-risk cutaneous squamous cell carcinoma.
Copy Number Alterations (CNAs) represent the most common genetic alterations identified in ovarian cancer cells, being responsible for the extensive genomic instability observed in this cancer. Here we report the identification of CNAs in a cohort of Italian patients affected by ovarian cancer performed by SNP-based array. Our analysis allowed ... More
Intranasal Administration of PACAP Is an Efficient Delivery Route to Reduce Infarct Volume and Promote Functional Recovery After Transient and Permanent Middle Cerebral Artery Occlusion.
AuthorsCherait A,Maucotel J,Lefranc B,Leprince J,Vaudry D
JournalFrontiers in endocrinology
PubMed ID33551991
Intranasal (IN) administration appears to be a suitable route for clinical use as it allows direct delivery of bioactive molecules to the central nervous system, reducing systemic exposure and sides effects. Nevertheless, only some molecules can be transported to the brain from the nasal cavity. This led us to compare ... More
Repeated Ethanol Exposure Alters DNA Methylation Status and Dynorphin/Kappa-Opioid Receptor Expression in Nucleus Accumbens of Alcohol-Preferring AA Rats.
AuthorsNiinep K,Anier K,Eteläinen T,Piepponen P,Kalda A
JournalFrontiers in genetics
PubMed ID34899839
Growing evidence suggests that epigenetic mechanisms, such as DNA methylation and demethylation, and histone modifications, are involved in the development of alcohol and drug addiction. However, studies of alcohol use disorder (AUD) that are focused on epigenetic DNA modifications and gene expression changes remain conflicting. Our aim was to study ... More
Displaced Myonuclei in Cancer Cachexia Suggest Altered Innervation.
JournalInternational journal of molecular sciences
PubMed ID32041358
An idiopathic myopathy characterized by central nuclei in muscle fibers, a hallmark of muscle regeneration, has been observed in cancer patients. In cancer cachexia skeletal muscle is incapable of regeneration, consequently, this observation remains unaccounted for. In C26-tumor bearing, cachectic mice, we observed muscle fibers with central nuclei in the ... More
Promiscuous Binding of Microprotein Mozart1 to γ-Tubulin Complex Mediates Specific Subcellular Targeting to Control Microtubule Array Formation.
AuthorsHuang TL,Wang HJ,Chang YC,Wang SW,Hsia KC
JournalCell reports
PubMed ID32610137
Mog1 knockout causes cardiac hypertrophy and heart failure by downregulating tbx5-cryab-hspb2 signalling in zebrafish.
When a T cell and an antigen-presenting cell form an immunological synapse, rapid dynein-driven translocation of the centrosome toward the contact site leads to reorganization of microtubules and associated organelles. Currently, little is known about how the regulation of microtubule dynamics contributes to this process. Here, we show that the ... More
Strategy to prevent epitope masking in CAR.CD19+ B-cell leukemia blasts.
Chimeric antigen receptor T-cells (CAR T-cells) for the treatment of relapsing/refractory B-cell precursor acute lymphoblastic leukemia have led to exciting clinical results. However, CAR T-cell approaches revealed a potential risk of CD19-/CAR+ leukemic relapse due to inadvertent transduction of leukemia cells. BACKGROUND: METHODS: We evaluated the impact of a high ... More
Ovarian transcriptomic signatures of zebrafish females resistant to different environmental perturbations.
AuthorsValdivieso A,Ribas L,Piferrer F
JournalJournal of experimental zoology. Part B, Molecular and developmental evolution
PubMed ID30945804
Assessing tissue transcription biomarkers of chronic rhinosinusitis: a comparison of sampling methodologies.
AuthorsHoggard M,Douglas RG,Taylor MW,Biswas K
JournalInternational forum of allergy & rhinology
PubMed ID32662249
Telomere length correlates with subtelomeric DNA methylation in long-term mindfulness practitioners.
Mindfulness and meditation techniques have proven successful for the reduction of stress and improvement in general health. In addition, meditation is linked to longevity and longer telomere length, a proposed biomarker of human aging. Interestingly, DNA methylation changes have been described at specific subtelomeric regions in long-term meditators compared to ... More
Effect of Bitis gabonica and Dendroaspis angusticeps snake venoms on apoptosis-related genes in human thymic epithelial cells.
JournalThe journal of venomous animals and toxins including tropical diseases
PubMed ID33402885
BACKGROUND: Certain environmental toxins permanently damage the thymic epithelium, accelerate immune senescence and trigger secondary immune pathologies. However, the exact underlying cellular mechanisms and pathways of permanent immune intoxication remain unknown. The aim of the present study was to demonstrate gene expressional changes of apoptosis-related cellular pathways in human thymic ... More
Regulation of Fibroblast Activation Protein by Transforming Growth Factor Beta-1 in Glioblastoma Microenvironment.
JournalInternational journal of molecular sciences
PubMed ID33494271
The proline-specific serine protease fibroblast activation protein (FAP) can participate in the progression of malignant tumors and represents a potential diagnostic and therapeutic target. Recently, we demonstrated an increased expression of FAP in glioblastomas, particularly those of the mesenchymal subtype. Factors controlling FAP expression in glioblastomas are unknown, but evidence ... More
Human Liver Stem Cell-Derived Extracellular Vesicles Target Hepatic Stellate Cells and Attenuate Their Pro-fibrotic Phenotype.
AuthorsChiabotto G,Ceccotti E,Tapparo M,Camussi G,Bruno S
JournalFrontiers in cell and developmental biology
PubMed ID34796180
Liver fibrosis occurs in response to chronic liver injury and is characterized by an excessive deposition of extracellular matrix. Activated hepatic stellate cells are primarily responsible for this process. A possible strategy to counteract the development of hepatic fibrosis could be the reversion of the activated phenotype of hepatic stellate ... More
Uncovering Tumour Heterogeneity through PKR and nc886 Analysis in Metastatic Colon Cancer Patients Treated with 5-FU-Based Chemotherapy.
AuthorsOrtega-García MB,Mesa A,Moya ELJ,Rueda B,Lopez-Ordoño G,García JÁ,Conde V,Redondo-Cerezo E,Lopez-Hidalgo JL,Jiménez G,Peran M,Martínez-González LJ,Del Val C,Zwir I,Marchal JA,García MÁ
JournalCancers
PubMed ID32045987
Colorectal cancer treatment has advanced over the past decade. The drug 5-fluorouracil is still used with a wide percentage of patients who do not respond. Therefore, a challenge is the identification of predictive biomarkers. The protein kinase R (PKR also called EIF2AK2) and its regulator, the non-coding pre-mir-nc886, have multiple ... More
Stenoparib, an Inhibitor of Cellular Poly(ADP-Ribose) Polymerase, Blocks Replication of the SARS-CoV-2 and HCoV-NL63 Human Coronaviruses In Vitro.
By late 2020, the coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), had caused tens of millions of infections and over 1 million deaths worldwide. A protective vaccine and more effective therapeutics are urgently needed. We evaluated a new poly(ADP-ribose) polymerase (PARP) inhibitor, stenoparib, ... More
The Q223R Polymorphism of the Leptin Receptor Gene as a Predictor of Weight Gain in Childhood Obesity and the Identification of Possible Factors Involved.
AuthorsMarcos-Pasero H,Aguilar-Aguilar E,Colmenarejo G,Ramírez de Molina A,Reglero G,Loria-Kohen V
JournalGenes
PubMed ID32429577
(1) Background: Childhood rapid weight gain during development has been postulated as a predictor of obesity. The objective of this study was to investigate the effect of single nucleotide polymorphisms (SNPs) on the annual weight gain and height growth, as well as identifying possible lifestyle factors involved. (2) Methods: As ... More
Impact of Acute Inflammation on Cytochromes P450 Activity Assessed by the Geneva Cocktail.
Cytochromes P450 (CYP) are subject to important interindividual variability in their activity due to genetic and environmental factors and some diseases. Limited human data support the idea that inflammation downregulates CYP activities. Our study aimed to evaluate the impact of orthopedic surgery (acute inflammation model) on the activity of six ... More
Expanding the Spectrum of FAT1 Nephropathies by Novel Mutations That Affect Hippo Signaling.
INTRODUCTION: Disease-causing mutations in the protocadherin FAT1 have been recently described both in patients with a glomerulotubular nephropathy and in patients with a syndromic nephropathy. METHODS: We identified 4 patients with FAT1-associated disease, performed clinical and genetic characterization, and compared our findings to the previously published patients. Patient-derived primary urinary ... More
Sex-Specific Regulation of Gene Expression Networks by Surfactant Protein A (SP-A) Variants in Alveolar Macrophages in Response to Klebsiella pneumoniae.
AuthorsThorenoor N,Kawasawa YI,Gandhi CK,Floros J
JournalFrontiers in immunology
PubMed ID32670284
Surfactant protein A (SP-A) in addition to its surfactant-related functions interacts with alveolar macrophages (AM), the guardian cells of innate immunity in the lungs, and regulates many of its functions under basal condition and in response to various pressures, such as infection and oxidative stress. The human SP-A locus consists ... More
NOS3 rs1799983 and rs2070744 polymorphisms and their association with advanced chronic kidney disease and coronary heart disease in Canarian population with type 2 diabetes.
AuthorsBoronat M,Tugores A,Saavedra P,Garay P,Bosch E,Lorenzo D,Ibarra A,García-Cantón C
JournalActa endocrinologica (Bucharest, Romania : 2005)
PubMed ID35747853
CONTEXT: Different polymorphisms of the endothelial nitric oxide synthase gene (NOS3) have been related to diabetic kidney disease. OBJECTIVE: To evaluate the association between advanced diabetic chronic kidney disease (ACKD) and the rs1799983 and rs2070744 poymorphisms of NOS3 in a population from the Gran Canaria island. DESIGN: Cross-sectional case-control study. ... More
Transmission of SARS-CoV-2 in Surgical Smoke during Laparoscopy: A Prospective, Proof-of-concept Study.
STUDY OBJECTIVE: There are growing concerns regarding the potential risk of coronavirus disease transmission during surgery and in particular during minimally invasive procedures owing to the aerosolization of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) particles. However, no study has demonstrated this hypothesis. Here, we aimed to investigate the presence ... More
Association between the TPMT*3C (rs1142345) Polymorphism and the Risk of Death in the Treatment of Acute Lymphoblastic Leukemia in Children from the Brazilian Amazon Region.
AuthorsCardoso de Carvalho D,Pereira Colares Leitão L,Mello Junior FAR,Vieira Wanderley A,Souza TP,Borges Andrade de Sá R,Cohen-Paes A,Rodrigues Fernandes M,Santos S,Salim Khayat A,Pimentel de Assumpção P,Pereira Carneiro Dos Santos N
JournalGenes
PubMed ID32992962
Acute lymphoblastic leukemia (ALL) is the leading cause of death from pediatric cancer worldwide. However, marked ethnic disparities are found in the treatment of childhood ALL with less effective results and higher mortality rates being obtained in populations with a high level of Native American ancestry. Genetic variations of the ... More
Cellular Gene Modulation of HIV-Infected CD4 T Cells in Response to Serial Treatment with the Histone Deacetylase Inhibitor Vorinostat.
Histone deacetylase inhibitors (HDACi) are the most widely studied HIV latency-reversing agents (LRAs). The HDACi suberoylanilide hydroxamic acid (vorinostat [VOR]) has been employed in several clinical HIV latency reversal studies, as well as in vitro models of HIV latency, and has been shown to effectively induce HIV RNA and protein ... More
Development and Application of a Transcriptomic Signature of Bioactivation in an Advanced In Vitro Liver Model to Reduce Drug-induced Liver Injury Risk Early in the Pharmaceutical Pipeline.
JournalToxicological sciences : an official journal of the Society of Toxicology
PubMed ID32559289
Expression analysis of porcine miR-33a/b in liver, adipose tissue and muscle and its potential role in fatty acid metabolism.
AuthorsCriado-Mesas L,Ballester M,Crespo-Piazuelo D,Passols M,Castelló A,Sánchez A,Folch JM
JournalPloS one
PubMed ID33497399
mir-33a and mir-33b are co-transcribed with the SREBF2 and SREBF1 transcription factors, respectively. The main role of SREBF1 is the regulation of genes involved in fatty acid metabolism, while SREBF2 regulates genes participating in cholesterol biosynthesis and uptake. Our objective was to study the expression of both miR-33a and miR-33b, ... More
Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA.
Patients with COVID-19 shed SARS-CoV-2 RNA in stool, sometimes well after their respiratory infection has cleared. This may be significant for patient health, epidemiology, and diagnosis. However, methods to preserve stool, and to extract and quantify viral RNA are not standardized. We test the performance of three preservative approaches at yielding detectable ... More
Sphingosine signaling dysfunction in airway cells as a potential contributor to progression from protracted bacterial bronchitis to bronchiectasis in children.
AuthorsHodge S,Macowan M,Liu H,Hamon R,Chen AC,Marchant JM,Pizzutto SJ,Upham JW,Chang AB
JournalPediatric pulmonology
PubMed ID32176839
Characterization of Reference Materials for Genetic Testing of CYP2D6 Alleles: A GeT-RM Collaborative Project.
Pharmacogenetic testing increasingly is available from clinical and research laboratories. However, only a limited number of quality control and other reference materials currently are available for the complex rearrangements and rare variants that occur in the CYP2D6 gene. To address this need, the Division of Laboratory Systems, CDC-based Genetic Testing ... More
Screening for Potential Biomarkers in Peripheral Blood From Miners Exposed to Radon Radiation.
JournalDose-response : a publication of International Hormesis Society
PubMed ID32165872
In this cohort study of 144 miners, 72 miners worked underground (the study group) and 72 miners worked aboveground (the control group). Based on questionnaire data and of radon concentration measurements, the cumulative radon exposure dose was calculated for each miner using the parameters recommended in International Commission on Radiological ... More
Pulmonary immune cell transcriptome changes in double-hit model of BPD induced by chorioamnionitis and postnatal hyperoxia.
BACKGROUND: Preterm infants with bronchopulmonary dysplasia (BPD) have lifelong increased risk of respiratory morbidities associated with environmental pathogen exposure and underlying mechanisms are poorly understood. The resident immune cells of the lung play vital roles in host defense. However, the effect of perinatal events associated with BPD on pulmonary-specific immune ... More
Interneuron Accumulation of Phosphorylated tau Impairs Adult Hippocampal Neurogenesis by Suppressing GABAergic Transmission.
Intracellular Toxic Advanced Glycation End-Products Promote the Production of Reactive Oxygen Species in HepG2 Cells.
AuthorsSakasai-Sakai A,Takata T,Takeuchi M
JournalInternational journal of molecular sciences
PubMed ID32660150
Hepatocyte cell death is a key process in the pathogenesis of nonalcoholic steatohepatitis (NASH). However, the factors responsible for and mechanisms underlying NASH-related cell death have not yet been elucidated in detail. We herein investigated the effects of intracellular glyceraldehyde (GA)-derived advanced glycation end-products (AGEs), named toxic AGEs (TAGE), on ... More
Methods for quick, accurate and cost-effective determination of the type 1 diabetes genetic risk score (T1D-GRS).
AuthorsLocke JM,Latten MJ,Datta RY,Wood AR,Crockard MA,Lamont JV,Weedon MN,Oram RA
JournalClinical chemistry and laboratory medicine
PubMed ID31665112
A hybrid implementation-effectiveness randomized trial of CYP2D6-guided postoperative pain management.
JournalGenetics in medicine : official journal of the American College of Medical Genetics
PubMed ID33420349
PURPOSE: Cytochrome P450 2D6 (CYP2D6) genotype-guided opioid prescribing is limited. The purpose of this type 2 hybrid implementation-effectiveness trial was to evaluate the feasibility of clinically implementing CYP2D6-guided post-surgical pain management and determine that such an approach did not worsen pain control. METHODS: Adults undergoing total joint arthroplasty were randomized ... More
Kahweol Found in Coffee Inhibits IL-2 Production Via Suppressing the Phosphorylations of ERK and c-Fos in Lymphocytic Jurkat Cells.
AuthorsPark JB
JournalJournal of dietary supplements
PubMed ID32583704
Modelling the Impact of Chronic Cigarette Smoke Exposure in Obese Mice: Metabolic, Pulmonary, Intestinal, and Cardiac Issues.
Unhealthy lifestyle choices, such as bad eating behaviors and cigarette smoking, have major detrimental impacts on health. However, the inter-relations between obesity and smoking are still not fully understood. We thus developed an experimental model of high-fat diet-fed obese C57BL/6 male mice chronically exposed to cigarette smoke. Our study evaluated ... More
Silencing of Aberrant Secretory Protein Expression by Disease-Associated Mutations.
AuthorsTikhonova EB,Karamysheva ZN,von Heijne G,Karamyshev AL
JournalJournal of molecular biology
PubMed ID31100385
Signal Recognition Particle (SRP) recognizes signal sequences of secretory proteins and targets them to the endoplasmic reticulum membrane for translocation. Many human diseases are connected with defects in signal sequences. The current dogma states that the molecular basis of the disease-associated mutations in the secretory proteins is connected with defects ... More
Molecular Changes in the Non-Inflamed Terminal Ileum of Patients with Ulcerative Colitis.
AuthorsLee HS,Vancamelbeke M,Verstockt S,Wilms T,Verstockt B,Sabino J,Ferrante M,Vermeire S,Cleynen I
JournalCells
PubMed ID32731480
Ulcerative colitis is a chronic inflammatory disease confined to the colon. Although the etiopathogenesis remains unknown, small bowel dysfunctions like histological and permeability alterations have been described in ulcerative colitis. We evaluated the molecular gene signature in the non-inflamed terminal ileum of 36 ulcerative colitis patients (7 active, with Mayo ... More
miR-150-PTPMT1-cardiolipin signaling in pulmonary arterial hypertension.
Circulating levels of endothelial miR-150 are reduced in pulmonary arterial hypertension (PAH) and act as an independent predictor of patient survival, but links between endothelial miR-150 and vascular dysfunction are not well understood. We studied the effects of endothelial miR-150 supplementation and inhibition in PAH mice and cells from patients ... More
Respiratory Microbial Co-infection With SARS-CoV-2.
AuthorsMassey BW,Jayathilake K,Meltzer HY
JournalFrontiers in microbiology
PubMed ID32983056
Co-infection with additional pathogens is a well-known feature of pandemics. We determined the prevalence and type of a wide variety of respiratory pathogens in 12,075 United States subjects tested for SARS-CoV-2 infection in March and April 2020. Infections with other respiratory pathogens, which on their own produce at least some ... More
Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study.
BACKGROUND: Small noncoding microRNA (miRNA) have regulatory functions in polycystic ovary syndrome (PCOS) that differ to those in women without PCOS. However, little is known about miRNA expression in women with PCOS who are not insulin resistant (IR). METHODS: Circulating miRNAs were measured using quantitative polymerase chain reaction (qPCR) in ... More
Metabolic Effects of Aripiprazole and Olanzapine Multiple-Dose Treatment in a Randomised Crossover Clinical Trial in Healthy Volunteers: Association with Pharmacogenetics.
INTRODUCTION: Aripiprazole and olanzapine are atypical antipsychotics. Both drugs can induce metabolic changes; however, the metabolic side effects produced by aripiprazole are more benign. The aim of the study was to evaluate if aripiprazole and olanzapine alter prolactin levels, lipid and glucose metabolism and hepatic, haematological, thyroid and renal function. ... More