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QuantStudio™ 12K Flex Real-Time PCR System, OpenArray™ block, Accufill™ System

Catalog number: 4471090

Applied Biosystems™  Related applications: Digital PCR | Genotyping & Genomic Profiling | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research. Note: this particular system includes an OpenArray block and AccuFill™ sample handling system. For a package that does not include an AccuFill system, please see Cat. No. 4472380.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray® components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

Flexibility & Scalability
Includes five interchangeable blocks: The QuantStudio™ 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan® array cards, and OpenArray® plates, for effortless scaling from 1 to 12,000 data points in a single 100 minute run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan® array cards and OpenArray® plates.
Compatible with a full range of TaqMan® assays: The QuantStudio™ 12K Flex System is optimized to enable clear, clean data for all TaqMan® assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.
Provides a seamless switch from qPCR to digital PCR: Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio® Digital PCR kits and DigitalSuite Software with the QuantStudio™ OpenArray ® block.
Delivers peace of mind: Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

Enhanced OptiFlex™ System: Building upon Applied Biosystems’ proprietary technology, the Enhanced OptiFlex™ System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single 100 minute run.
Unprecedented coverage and sample throughput: The OpenArray® format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.
Simple OpenArray® workflow: QuantStudio™ Open Array® plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio™ 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.
Tools to maximize your resources: New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

QuantStudio™ 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments

Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.
High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.
DigitalSuite Software v1.0: For OpenArray™ block users needing sensitivity for rare targets and precision to measure small differences in target abundance.
ExpressionSuite software v1.0: our latest stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray® plates.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.


Format: OpenArray® Plate
Weight: 69 kg (152 lbs)
Capacity: 4 OpenArray® (3072 Through-Hole) Plates
Dimensions: 51 cm (W) x 74 cm (H) x 67 cm (D)
Power Limit: 60 Hz
Sensitivity: Down to 1 copy
Block Format: Interchangeable
Product Size: 1 system
Dynamic Range: Up to 7 logs of linear dynamic range (OpenArray™)
Input Voltage: 100-240 VAC
Display Interface: Touchscreen
External Computer: Computer and Monitor
Other Format Options: 96-well Fast Block Upgrade Option, 384-well Block Upgrade Option, TaqMan® Array Card Block Upgrade Option, 96-well Standard Block Upgrade Option
For Use With (Equipment): QuantStudio™


Manuals & protocols