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Invitrogen
Phospho-IKK alpha (Ser176, Ser180) Polyclonal Antibody
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FIGURE: 1 / 5
Phospho-IKK alpha (Ser176, Ser180) Antibody (44-714) in ICC/IF
Immunofluorescence analysis of Phospho-IKK alpha (Ser176, Ser180) was done on 70% confluent log phase HeLa cells. The cells were treated for 20 minutes with 50 ng/mL of TNF-Alpha and fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-IKK alpha (Ser176, Ser180) Rabbit... View More
Product Details
44-714
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IKK-alpha that contains serine 176 and serine 180.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
CHUK (IKK alpha) is a serine/threonine kinase involved in signal transduction and cellular communication. It belongs to the cytokine activated protein complex that is an inhibitor of the NF-kappa B complex. IKK alpha along with IKK beta and a regulatory protein NEMO (IKK gamma) phosphorylates IkB proteins which targets the later for ubiquitin mediated degradation. It is involved with several cellular processes with extensive studies on its role in inflammation and cell survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; FLJ40509; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; ikappaB kinase; IkB kinase alpha subunit; IKBKB; IKK-1; IKK-a kinase; IKK-B; IKK-beta; IKK2; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit alpha; kinase beta; MGC131801; NFKBIKA; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; protein kinase; TCF-16; Transcription factor 16
Gene Aliases: BPS2; CHUK; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16
UniProt ID: (Human) O15111
Entrez Gene ID: (Human) 1147
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
IkappaB kinase activity
kinase activity
transferase activity
protein homodimerization activity
macromolecular complex binding
protein heterodimerization activity
scaffold protein binding
transferrin receptor binding
pattern recognition receptor signaling pathway
skeletal muscle contraction
regulation of transcription from RNA polymerase II promoter
inflammatory response
immune response
I-kappaB kinase/NF-kappaB signaling
response to xenobiotic stimulus
response to virus
response to toxic substance
anatomical structure morphogenesis
response to acetate
negative regulation of NF-kappaB transcription factor activity
positive regulation of interferon-alpha production
response to hydroperoxide
tumor necrosis factor-mediated signaling pathway
toll-like receptor 4 signaling pathway
NIK/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to amino acid
innate immune response
positive regulation of transcription from RNA polymerase II promoter
positive regulation of NF-kappaB transcription factor activity
striated muscle cell differentiation
response to cholecystokinin
cellular response to tumor necrosis factor
cellular response to virus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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