Search
Invitrogen
Phospho-Paxillin (Tyr118) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 49
Phospho-Paxillin (Tyr118) Antibody (44-722G) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of COS-7 (Lane 1), COS-7 treated with 100 uM Pervanadate for 1 hour (Lane 2), A431 (Lane 3), A431 treated with 100 uM Pervanadate for 1 hour (Lane 4), HeLa (Lane 5) and HeLa treated with 100 uM Pervanadate for 1 hour (Lane 6).The blots were probed with Anti-Phospho-Paxillin pTyr118 Rabbit Polyclonal Antibody (Product # 44-722G, 1:500 ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
44-722G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Chicken,
Dog,
Human,
Mouse,
Pig,
Rat,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains tyrosine 118.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 118.
Positive controls used with this antibody in western blotting were normal mouse mammary gland (NMuMG) cells, NMuMG transfected with EGFP-tagged paxillin +/- TGFbeta, and human epidermoid carcinoma cells (A431) +/- EGF.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ16691; hCG_1778014; PAX-1; PAXI; paxillin alpha; testicular tissue protein Li 134; unnamed protein product
Gene Aliases: Pax
UniProt ID: (Human) P49023, (Mouse) Q8VI36
Entrez Gene ID: (Human) 5829, (Mouse) 19303
cell adhesion
signal transduction
signal complex assembly
transforming growth factor beta receptor signaling pathway
cell migration
substrate adhesion-dependent cell spreading
cellular response to reactive oxygen species
endothelial cell migration
positive regulation of stress fiber assembly
growth hormone receptor signaling pathway
MAPK cascade
cytoskeleton organization
integrin-mediated signaling pathway
regulation of cell shape
peptidyl-tyrosine phosphorylation
lamellipodium assembly
lipopolysaccharide-mediated signaling pathway
positive regulation of angiogenesis
positive regulation of protein kinase activity
focal adhesion assembly
branching morphogenesis of an epithelial tube
positive regulation of substrate adhesion-dependent cell spreading
response to peptide
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support