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Invitrogen
Phospho-p70 S6 Kinase (Thr229) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-p70 S6 Kinase (Thr229) Antibody (44-918G) in ICC/IF
Immunofluorescence analysis of Phospho-p70S6K (Product # 44-918G) was done on 70% confluent log phase A431 cells treated with 200 ng of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-p70S6K (Thr229) Rabbit Polyclonal Antibody (Pr... View More
Product Details
44-918G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human p70-S6K that contains threonine 229. The sequence is conserved in rat.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 70 kDa ribosomal protein S6 kinase 1; EC 2.7.11.1; kinase p70S6K; KS6B1; p70 p70-S6K; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70S6K-alpha; P70S6K1; p80-S6K 1; p85 p85S6K; Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kDa, polypeptide 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; unnamed protein product
Gene Aliases: p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; RPS6KB1; S6K; S6K-beta-1; S6K1; STK14A
UniProt ID: (Human) P23443
Entrez Gene ID: (Human) 6198
autophagosome assembly
G1/S transition of mitotic cell cycle
behavioral fear response
cytoplasmic translation
regulation of translation
ubiquitin-dependent protein catabolic process
apoptotic process
signal transduction
germ cell development
insulin receptor signaling pathway
negative regulation of autophagy
positive regulation of autophagy
peptidyl-serine phosphorylation
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
response to nutrient levels
cellular response to nutrient levels
cellular response to nutrient
TOR signaling
cellular response to insulin stimulus
TORC1 signaling
TORC2 signaling
negative regulation of apoptotic process
protein kinase B signaling
long-chain fatty acid import
positive regulation of translation
positive regulation of mitotic cell cycle
positive regulation of translational initiation
negative regulation of insulin receptor signaling pathway
phosphatidylinositol-mediated signaling
modulation of synaptic transmission
positive regulation of protein kinase B signaling
cellular response to interferon-gamma
cellular response to growth factor stimulus
cellular response to dexamethasone stimulus
negative regulation of TORC2 signaling
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
negative regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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