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Invitrogen
CD85g (ILT7) Monoclonal Antibody (eBio17G10.2 (17G10.2)), PerCP-Cyanine5.5, eBioscience™
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FIGURE: 1 / 2
CD85g (ILT7) Antibody (45-5179-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD123 FITC (Product # 11-1239-42) and Mouse IgG1 K Isotype Control PerCP-Cyanine5-5 (Product # 45-4714-82) (left) or Anti-Human CD85g (ILT7) PerCP-Cyanine5-5 (right). Cells in the monocyte gate were used for analysis.
Product Details
45-5179-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio17G10.2 (17G10.2)
Conjugate
Excitation/Emission Max
489/679 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBio17G10.2 monoclonal antibody reacts with human immunoglobulin-like transcript 7 (ILT7), also known as CD85g. ILT7 is part of the leukocyte immunoglobulin-like receptor (LIR) gene family and associates with FcεR1gamma to form a functional complex which transduces ITAM-mediated signals that negatively modulates TLR-induced type I IFN production by human plasmacytoid dendritic cells. ILT7 is expressed exclusively on plasmacytoid dendritic cells and is absent in monocyte derived DCs. Treatment with IL-3 or CpG causes a decrease in expression.
Double staining of human PBMCs with eBio17G10.2 and anti-pDC marker BDCA2 yields virtually complete co-staining. Cross linking of ILT7 with eBio17G10.2 antibody causes reduced transcription of IFN-alpha1, IFN-alpha2, IFN-alpha4 and IFN-beta.
Applications Reported: This eBio17G10.2 (17G10.2) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio17G10.2 (17G10.2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ILT7 is a pDC-specific IgG-like transcript and is a member of the leukocyte immunoglobulin-like receptor gene family. This receptor, in complex with FcER1a, transduces ITAM-mediated signals which negatively modulate type I IFN production by pDC. ILT7 is thus a potential target to regulate Type 1 IFN production by pDC, which play a major role during viral infection, auto-immune diseases and anti-cancer immunity. Additionally, ILT7, along with CD123 and BDCA-2, can be used to identify or classify subsets of pDCalong with TLR expression and TLR mediated immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD85 antigen-like family member G; CD85g; ILT-7; immunoglobulin superfamily member; Immunoglobulin-like transcript 7; leucocyte Ig-like receptor A4; Leukocyte immunoglobulin-like receptor subfamily A member 4; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member 4
Gene Aliases: CD85g; ILT7; LILRA4
UniProt ID: (Human) P59901
Entrez Gene ID: (Human) 23547
immune system process
immune response-regulating signaling pathway
cell surface receptor signaling pathway
cytokine-mediated signaling pathway
negative regulation of interferon-alpha production
negative regulation of tumor necrosis factor production
negative regulation of toll-like receptor 7 signaling pathway
negative regulation of toll-like receptor 9 signaling pathway
Fc-epsilon receptor signaling pathway
innate immune response
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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