DyLight™ 633 Maleimide

Catalog number: 46613

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Description

Thermo Scientific DyLight 633 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 633 used to fluorescently label sulfhydryl-containing peptides, proteins, and other biomolecular probes.

DyLight 633 fluoresces red and has physical properties comparable to other 633 dyes, including Alexa Fluor™ 633, over a broad pH range (pH 4–9). The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.

Features of DyLight 633 Maleimide:

High performance—DyLight 633 shows brighter fluorescence than Alexa Fluor 633
Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:
• Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies

DyLight 633 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5–7.5. Learn more about maleimide chemistry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity: Thiol
Excitation⁄Emission (nm): 638⁄658
Label or Dye: DyLight™ 633
Product Line: DyLight™
Product Size: 1 mg
Solubility: DMF (Dimethylformamide)

Contents & storage

Store desiccated at -20°C. Shipped at ambient temperature.

Documents

Manuals & protocols