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Antibody Testing Data (1)

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IL-13 Antibody (48-7136-42) in Flow

Normal human peripheral blood cells were stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) and then stained with CD4 Monoclonal Antibody, FITC (Product # 11-0049-42). Cells were then stained intracellularly using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with Mouse IgG2b kappa Isotype Control, eFluor 450 (Product # 48-4732-82) (left) or IL-13 Monoclonal Antibody, eFluor 450 (right). Cells in the lymphocyte gate were used f... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Product Details

48-7136-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

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Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

85BRD

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Ambient (domestic); Wet ice (international)

AB_2784729

Product Specific Information

Description: The 85BRD monoclonal antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.

Applications Reported: This 85BRD antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 85BRD antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins." This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; ILN; Interleukin; Interleukin13; MGC116786; MGC116788; MGC116789; NC30; NC30 antibody; P600 antibody

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Gene Aliases: IL-13; P600

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UniProt ID: (Human) P35225

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Entrez Gene ID: (Human) 3596

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Function(s)

cytokine activity cytokine receptor binding interleukin-13 receptor binding protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-13 Monoclonal Antibody (85BRD), eFluor™ 450, eBioscience™

IL-13 Antibody (48-7136-42) in Flow

Product Details

48-7136-42

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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