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Invitrogen
Doublecortin Polyclonal Antibody
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FIGURE: 1 / 11
Doublecortin Antibody (48-1200) in ICC/IF
Immunofluorescence analysis of Doublecortin was performed using 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Doublecortin/DCX Rabbit Polyclonal Antibody (Product # 48-1200) at 2 µg/mL in 0.1% BSA and incubated for 3 h... View More
Product Details
48-1200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the C-terminal region of the human doublecortin protein
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: doublecortex; Doublin; Lis-X; LIS-XD; Lissencephalin-X; lissencephaly, X-linked (doublecortin); Neuronal migration protein doublecortin; OTTHUMP00000023860; OTTHUMP00000216316
Gene Aliases: DBCN; Dbct; DC; Dcn; DCX; LISX; SCLH; XLIS
UniProt ID: (Human) D3DUY9, (Mouse) O88809
Entrez Gene ID: (Human) 1641, (Rat) 84394, (Mouse) 13193
neuron migration
brain development
central nervous system projection neuron axonogenesis
intracellular signal transduction
axon extension
dendrite morphogenesis
response to electrical stimulus
multicellular organism development
nervous system development
peptidyl-serine phosphorylation
cell differentiation
protein autophosphorylation
central nervous system development
hippocampus development
layer formation in cerebral cortex
pyramidal neuron development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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