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Invitrogen
VGLUT1 Polyclonal Antibody
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FIGURE: 1 / 24
VGLUT1 Antibody (48-2400) in ICC/IF
Immunofluorescence analysis of VGLUT1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VGLUT1 Rabbit Polyclonal Antibody (Product # 48-2400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product... View More
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20240507?time=20240507 "VGLUT1 Antibody (48-2400) in ICC/IF")
Product Details
48-2400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the internal region of the mouse VGLUT1 protein (Accession# NP_892038, Q3TXX4), which is 93% homologous to human and rat.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for the VGLUT1 protein. On western blots, it identifies the target band at ~55 kDa. Reactivity has been confirmed with mouse brain homogenates by western blotting, with mouse hippocampus frozen sections by immunohistochemistry, and in immunofluorescence analysis with human glutamate neurons derived from iPSCs.
Target Information
A Na(+)-dependent inorganic phosphate cotransporter; may be involved in neuronal Na+ transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: brain-specific Na(+)-dependent inorganic phosphate cotransporter; brain-specific Na-dependent inorganic phosphate cotransporter; SLC17A1; SLC17A1/VGLUT1; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; solute carrier family 17 (vesicular glutamate transporter), member 7; solute carrier family 17 member 7; unnamed protein product; VGlU1; VGLUT 1; VGLUT1v
Gene Aliases: 2900052E22Rik; BNPI; VGLUT1
UniProt ID: (Human) Q9P2U7, (Rat) Q62634, (Mouse) Q3TXX4
Entrez Gene ID: (Human) 57030, (Rat) 116638, (Mouse) 72961
chloride channel activity
L-glutamate transmembrane transporter activity
neurotransmitter transporter activity
sodium:phosphate symporter activity
extracellular-glutamate-gated chloride channel activity
secondary active transmembrane transporter activity
potassium:proton antiporter activity
transmembrane transporter activity
phosphate ion uniporter activity
L-glutamate uniporter activity
protein binding
neurotransmitter uptake
neural retina development
potassium ion transport
phosphate ion transport
chloride transport
chemical synaptic transmission
long-term memory
L-glutamate transport
synaptic transmission, glutamatergic
phosphate ion transmembrane transport
sodium ion transmembrane transport
sodium-dependent phosphate transport
regulation of synapse structure or activity
L-glutamate import
phosphate ion homeostasis
transmembrane transport
excitatory postsynaptic potential
potassium ion transmembrane transport
synaptic vesicle lumen acidification
neurotransmitter loading into synaptic vesicle
regulation of synaptic vesicle endocytosis
chloride transmembrane transport
neurotransmitter transport
hydrogen ion transmembrane transport
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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