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Invitrogen
Estrogen Receptor alpha Monoclonal Antibody
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FIGURE: 1 / 4
Estrogen Receptor alpha Antibody (49-1002) in ICC/IF
COS-7 cells transiently overexpressing human ERα (left panel) or ERβ1 (right panel) were both labeled with the anti-ERα antibody followed by biotinylated secondary antibody and peroxidase-labeled avidin. The monoclonal antibody directed against ERα (clone: mAN1, Product # 49-1002) is used at 15 µg⁄ml. The antibody is clearly recognizing the ERα isoform and not ERβ
Product Details
49-1002
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Immunogen
NH2 terminus of the human estrogen receptor alpha using a peptide antigen (Q19-K32) conjugated to KLH
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
50 µg
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686N23123; E2 receptor alpha; ER; ER delta E7 isoform; ER-alpha; ER-alpha; del.3*,+1*,4,5,6,7* variant; ER-alpha; del.3*,4,5,6,7*/770 variant; ER-alpha; del.3*,4,5,6,7,8*/862 variant; ER-alpha; del.4*,5*,7 variant; ER-alpha; del.4*,5,6*/548 variant; ER-alpha; del.4*,5,6,7*/714 variant; ER-alpha; del.4*,5,6,7,8*/754 variant; ER-alpha; estrogen receptor alpha delta 3*,4,5,6,7*/819 cingulate gyrus isoform; ER-alpha; estrogen receptor alpha delta 3*,4,5,6,7*/833 superior frontal gyrus isoform; ER-alpha; estrogen receptor alpha delta 4*,5,6,7*/747 substantia nigra isoform; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor-related protein; hER-alpha36; lacks a part of the exon 3, exons 4-6, and part of exon 7; contains an 822 base in frame deletion; lacks most of the exon 3, exons 4-7, and part of exon 8; lacks most of the exon 4, exons 5-6, and part of exon 7; lacks the end of the exon 3, exons 4-7, and part of exon 8; MB1; Nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha; receptor; RP1-130E4.1; steroid hormone receptor; unnamed protein product
Gene Aliases: ER; Era; ESR; ESR1; ESRA; ESTRR; NR3A1
UniProt ID: (Human) P03372
Entrez Gene ID: (Human) 2099
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
TFIIB-class transcription factor binding
transcription cofactor binding
transcription corepressor binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
steroid binding
protein binding
calmodulin binding
beta-catenin binding
zinc ion binding
lipid binding
TBP-class protein binding
enzyme binding
protein kinase binding
nitric-oxide synthase regulator activity
estrogen receptor activity
estrogen receptor binding
estrogen response element binding
identical protein binding
sequence-specific DNA binding
metal ion binding
ATPase binding
14-3-3 protein binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
antral ovarian follicle growth
epithelial cell development
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
signal transduction
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
androgen metabolic process
male gonad development
negative regulation of gene expression
intracellular steroid hormone receptor signaling pathway
intracellular estrogen receptor signaling pathway
response to estradiol
regulation of toll-like receptor signaling pathway
negative regulation of smooth muscle cell apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
response to estrogen
positive regulation of nitric oxide biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
fibroblast proliferation
positive regulation of fibroblast proliferation
stem cell differentiation
regulation of inflammatory response
positive regulation of nitric-oxide synthase activity
positive regulation of sequence-specific DNA binding transcription factor activity
RNA polymerase II transcriptional preinitiation complex assembly
uterus development
vagina development
prostate epithelial cord elongation
prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis
regulation of branching involved in prostate gland morphogenesis
mammary gland branching involved in pregnancy
mammary gland alveolus development
epithelial cell proliferation involved in mammary gland duct elongation
protein localization to chromatin
cellular response to estrogen stimulus
cellular response to estradiol stimulus
cellular response to oxygen-containing compound
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
regulation of epithelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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