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          • Primary Antibodies ›
          • mTOR Antibodies

          Invitrogen

          Phospho-mTOR (Ser2448) Monoclonal Antibody (MRRBY), eFluor™ 660, eBioscience™

          4 Published Figures
          7 References
          View all (66) mTOR antibodies

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          Cite Phospho-mTOR (Ser2448) Monoclonal Antibody (MRRBY), eFluor™ 660, eBioscience™

          Additional Information:
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          • Antibody Testing Data (1)
          • Published Figures (4)
          Phospho-mTOR (Ser2448) Antibody in Flow Cytometry (Flow)
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          Phospho-mTOR (Ser2448) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          Phospho-mTOR (Ser2448) Antibody (50-9718-41) in Flow

          TOP: Mouse splenocytes were unstimulated (left) or stimulated with Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82) plus Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) (right) for 48 hours. The cells were then intracellularly stained with Anti-Mouse CD3e PE (Product # 12-0031-82) and Anti-Human/Mouse phospho-mTOR (S2448) eFluor 660 using the Intracellular Fixation & Permeab... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-mTOR (Ser2448) Antibody in Flow Cytometry (Flow)
          Phospho-mTOR (Ser2448) Antibody in Flow Cytometry (Flow)
          Phospho-mTOR (Ser2448) Antibody in Flow Cytometry (Flow)
          Phospho-mTOR (Ser2448) Antibody in Flow Cytometry (Flow)
          Phospho-mTOR (Ser2448) Antibody in Flow Cytometry (Flow)

          Product Details

          50-9718-41

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          5 µL (0.5 µg)/test
          View 5 publications 5 publications
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG2a, kappa

          Recommended Isotype Control

          Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 660, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          MRRBY

          Conjugate

          eFluor™ 660 eFluor™ 660 eFluor™ 660
          • eFluor 450
          • PE 
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Request custom conjugation

          Excitation/Emission Max

          651/668 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2574351

          Product Specific Information

          Description: This MRRBY monoclonal antibody recognizes human and mouse mammalian target of rapamycin (also known as mTOR, FRAP, RAFT) when phosphorylated on S2448. mTOR is a serine/threonine protein kinase that functions as an ATP and amino acid sensor as well as to balance nutrient availability with cell growth, proliferation, motility, survival, protein synthesis, and transcription. Activated mTOR increases production of enzymes necessary for glycolysis and controls the uptake of glucose and other nutrients. Increased glucose uptake and metabolism helps fulfill the energy needs for mTOR-driven cell growth and proliferation. When sufficient nutrients are available, mTOR transmits a positive signal to p70 S6 kinase and participates in the inactivation of the eIF4E inhibitor, 4E-BP1. mTOR is phosphorylated at S2448 via the PI3 kinase/Akt signaling pathway and is autophosphorylated at Ser2481. Due to its critical role in regulation of cell growth, survival, and metabolism, and because it is often abnormally regulated in tumors, mTOR is under investigation as a potential target for anti-cancer therapy.

          Applications Reported:This MRRBY antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

          Applications Tested: This MRRBY antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the BestProtocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.

          eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

          Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; mTORC1; Rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR; Tyrosine-protein kinase mTOR; unnamed protein product

          View more View less

          Gene Aliases: 2610315D21Rik; AI327068; flat; FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS

          View more View less

          UniProt ID: (Human) P42345, (Mouse) Q9JLN9

          View more View less

          Entrez Gene ID: (Human) 2475, (Mouse) 56717

          View more View less

          Function(s)
          nucleotide binding inositol hexakisphosphate binding RNA polymerase III type 1 promoter sequence-specific DNA binding RNA polymerase III type 2 promoter sequence-specific DNA binding RNA polymerase III type 3 promoter sequence-specific DNA binding TFIIIC-class transcription factor binding protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity protein binding ATP binding kinase activity transferase activity identical protein binding ribosome binding ion channel binding macromolecular complex binding phosphoprotein binding protein serine kinase activity RNA polymerase III type 1 promoter DNA binding RNA polymerase III type 2 promoter DNA binding RNA polymerase III type 3 promoter DNA binding drug binding transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor protein domain specific binding protein dimerization activity non-receptor serine/threonine protein kinase protein modifying enzyme
          Process(es)
          autophagosome assembly regulation of cell growth cytoplasmic translation T-helper 1 cell lineage commitment heart morphogenesis heart valve morphogenesis energy reserve metabolic process 'de novo' pyrimidine nucleobase biosynthetic process regulation of translational initiation ubiquitin-dependent protein catabolic process inflammatory response cellular response to DNA damage stimulus cytoskeleton organization lysosome organization germ cell development positive regulation of cell proliferation insulin receptor signaling pathway regulation of cell size cellular response to starvation response to heat response to virus post-embryonic development positive regulation of biosynthetic process regulation of autophagy negative regulation of autophagy positive regulation of autophagy positive regulation of lamellipodium assembly positive regulation of epithelial to mesenchymal transition positive regulation of myotube differentiation macroautophagy regulation of macroautophagy negative regulation of macroautophagy regulation of lipid metabolic process neuronal action potential cell projection organization positive regulation of cell growth positive regulation of actin filament polymerization SCF-dependent proteasomal ubiquitin-dependent protein catabolic process T cell costimulation ruffle organization regulation of myelination protein destabilization response to nutrient levels cellular response to nutrient levels cellular response to nutrient TOR signaling response to insulin cellular response to insulin stimulus regulation of actin cytoskeleton organization calcineurin-NFAT signaling cascade cellular response to amino acid starvation multicellular organism growth TORC1 signaling TORC2 signaling regulation of circadian rhythm negative regulation of apoptotic process response to amino acid anoikis protein kinase B signaling positive regulation of cell differentiation regulation of osteoclast differentiation positive regulation of translation negative regulation of cell size positive regulation of glycolytic process negative regulation of innate immune response positive regulation of transcription from RNA polymerase III promoter negative regulation of translational initiation positive regulation of translational initiation negative regulation of insulin receptor signaling pathway positive regulation of lipid biosynthetic process behavioral response to pain oligodendrocyte differentiation positive regulation of oligodendrocyte differentiation cardiac muscle tissue development protein stabilization voluntary musculoskeletal movement positive regulation of multicellular organismal process positive regulation of stress fiber assembly positive regulation of keratinocyte migration regulation of protein kinase B signaling positive regulation of protein kinase B signaling cardiac cell development cardiac muscle cell development cardiac muscle contraction cellular response to methionine positive regulation of SCF-dependent proteasomal ubiquitin-dependent catabolic process negative regulation of calcineurin-NFAT signaling cascade cellular response to amino acid stimulus cellular response to leucine cellular response to hypoxia cellular response to osmotic stress regulation of cellular response to stress regulation of membrane permeability vascular endothelial cell response to laminar fluid shear stress antibacterial innate immune response regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of wound healing, spreading of epidermal cells negative regulation of TORC2 signaling regulation of locomotor rhythm negative regulation of TORC1 signaling positive regulation of TORC1 signaling positive regulation of cytoplasmic translational initiation regulation of lysosome organization negative regulation of lysosome organization positive regulation of pentose-phosphate shunt cellular response to leucine starvation negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of cytoplasmic translation regulation of autophagosome assembly double-strand break repair via homologous recombination positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation regulation of glycogen biosynthetic process regulation of carbohydrate metabolic process protein phosphorylation response to stress positive regulation of gene expression cell growth phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation regulation of fatty acid beta-oxidation regulation of response to food regulation of GTPase activity regulation of carbohydrate utilization regulation of protein kinase activity protein autophosphorylation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of NFAT protein import into nucleus
          It has to be done as per old AB suggested Products section.

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