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Invitrogen

VEGF-165 Polyclonal Antibody, Biotin, PeproTech®

1 Published Figure
2 References
View all (15) VEGF-165 antibodies

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Additional Information:

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VEGF-165 Polyclonal Antibody, Biotin, PeproTech®

Product Details

500-P10BT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

Flow Cytometry (Flow)

-
View 1 publication 1 publication

ELISA (ELISA)

0.25-1.0 µg/mL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived, 38.2 kDa Recombinant Human VEGF 165
3D Epitope / Immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

25 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929241

Product Specific Information

AA Sequence of recombinant protein: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human VEGF 165 (500-P10) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human VEGF 165.

Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P10BT-1MG will be provided as 2 x 500 µg

Target Information

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: growth factor; H-VEGF-165; M-VEGF-165; vascular endothelial growth factor; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor; vascular permeability factor precursor; VEGF121; VEGF165

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Gene Aliases: L-VEGF; MVCD1; VEGF; VPF

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UniProt ID: (Human) P15692

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Entrez Gene ID: (Human) 7422

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Function(s)
fibronectin binding cytokine activity platelet-derived growth factor receptor binding vascular endothelial growth factor receptor binding protein binding growth factor activity heparin binding transmembrane receptor protein tyrosine kinase activator activity neuropilin binding chemoattractant activity identical protein binding vascular endothelial growth factor receptor 1 binding vascular endothelial growth factor receptor 2 binding receptor agonist activity extracellular matrix binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter angiogenesis ovarian follicle development patterning of blood vessels vasculogenesis response to hypoxia in utero embryonic development kidney development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation sprouting angiogenesis cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation positive regulation of receptor internalization immune system process basophil chemotaxis positive regulation of leukocyte migration heart morphogenesis outflow tract morphogenesis coronary vein morphogenesis regulation of transcription from RNA polymerase II promoter phospholipase C-activating G-protein coupled receptor signaling pathway nervous system development mesoderm development lactation positive regulation of cell proliferation regulation of cell shape positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of epithelial to mesenchymal transition monocyte differentiation macrophage differentiation lung development positive regulation of cell migration epithelial cell differentiation post-embryonic camera-type eye development retinal ganglion cell axon guidance positive regulation of protein complex assembly positive regulation of protein autophosphorylation tube formation endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus lymph vessel morphogenesis vascular endothelial growth factor receptor-2 signaling pathway positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway vascular endothelial growth factor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway VEGF-activated neuropilin signaling pathway eye photoreceptor cell development negative regulation of apoptotic process positive regulation of vascular permeability positive regulation of I-kappaB kinase/NF-kappaB signaling surfactant homeostasis positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway cell maturation camera-type eye morphogenesis positive regulation of axon extension involved in axon guidance artery morphogenesis positive regulation of epithelial cell proliferation positive chemotaxis positive regulation of positive chemotaxis induction of positive chemotaxis positive regulation of focal adhesion assembly positive regulation of protein kinase B signaling cardiac muscle cell development primitive erythrocyte differentiation mammary gland alveolus development positive regulation of mast cell chemotaxis cardiac vascular smooth muscle cell development coronary artery morphogenesis vascular wound healing positive regulation of ERK1 and ERK2 cascade cellular response to hypoxia dopaminergic neuron differentiation commissural neuron axon guidance positive regulation of cell migration involved in sprouting angiogenesis positive regulation of branching involved in ureteric bud morphogenesis motor neuron migration cellular stress response to acid chemical positive regulation of cold-induced thermogenesis negative regulation of nitric oxide-cGMP mediated signal transduction positive regulation of vascular endothelial growth factor signaling pathway positive regulation of trophoblast cell migration negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to early endosome negative regulation of establishment of endothelial barrier negative regulation of adherens junction organization positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis positive regulation of sprouting angiogenesis positive regulation of epithelial tube formation positive regulation of blood vessel branching negative regulation of blood-brain barrier permeability negative regulation of cell-cell adhesion mediated by cadherin positive regulation of DNA biosynthetic process positive regulation of endothelial cell chemotaxis
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