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Invitrogen

IL-12 p70 Polyclonal Antibody, PeproTech®

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IL-12 p70 Polyclonal Antibody, PeproTech®

Product Details

500-P155G-100UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.5-2.0 µg/mL
View 2 publications 2 publications

Neutralization (Neu)

-
View 2 publications 2 publications

In vitro Assay (In vitro)

-
View 5 publications 5 publications

Immunostaining (IS)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

CHO cells-derived Recombinant Murine IL-12 p70
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929522

Product Specific Information

AA Sequence of recombinant protein: p35 subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine IL-12. Anti-Murine IL-12-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-12 matrix.

Sandwich ELISA: To detect Murine IL-12 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-12 (500-P155GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-12.

Western Blot: To detect Murine IL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 20C2; CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 35 kDa subunit; cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; IL-12 p35 subunit; IL-12 p40 subunit; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-23 subunit p40; IL12A/B; IL12AB; ILN; Interleukin; interleukin 12a p35 subunit; interleukin-12 p35 subunit; interleukin-12 p40 subunit; Interleukin-12 p70; MGC151228; MGC151232; unnamed protein product

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Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; Il12p40; Ll12a; p35; p40

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UniProt ID: (Mouse) P43431, (Mouse) P43432

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Entrez Gene ID: (Mouse) 16159, (Mouse) 16160

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Function(s)
cytokine activity interleukin-12 receptor binding protein binding growth factor activity interleukin-12 beta subunit binding interleukin-27 binding protein heterodimerization activity cytokine receptor activity cytokine receptor binding interleukin-12 alpha subunit binding identical protein binding macromolecular complex binding interleukin-23 receptor binding
Process(es)
positive regulation of leukocyte mediated cytotoxicity positive regulation of T cell mediated cytotoxicity positive regulation of defense response to virus by host natural killer cell activation involved in immune response positive regulation of lymphocyte mediated immunity positive regulation of T-helper 1 type immune response positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target negative regulation of inflammatory response to antigenic stimulus cell surface receptor signaling pathway JAK-STAT cascade cell proliferation response to UV-B cell migration natural killer cell activation negative regulation of interleukin-10 production negative regulation of interleukin-17 production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell activation positive regulation of natural killer cell proliferation positive regulation of mononuclear cell proliferation positive regulation of smooth muscle cell apoptotic process interleukin-12-mediated signaling pathway interleukin-23-mediated signaling pathway T-helper cell differentiation positive regulation of T cell proliferation positive regulation of activated T cell proliferation defense response to protozoan T-helper 1 cell differentiation positive regulation of osteoclast differentiation positive regulation of cell adhesion positive regulation of JAK-STAT cascade negative regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation negative regulation of protein secretion regulation of inflammatory response defense response to Gram-negative bacterium positive regulation of NK T cell activation positive regulation of NK T cell proliferation defense response to virus cellular response to lipopolysaccharide cellular response to interferon-gamma negative regulation of vascular endothelial growth factor signaling pathway negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis positive regulation of T-helper 17 type immune response immune response response to virus response to lipopolysaccharide T-helper 1 cell activation positive regulation of tyrosine phosphorylation of STAT protein positive regulation of T cell differentiation positive regulation of natural killer cell mediated cytotoxicity defense response to Gram-positive bacterium extrinsic apoptotic signaling pathway cellular response to virus positive regulation of dendritic cell chemotaxis
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