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Invitrogen
IL-28A Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
IL-28A Antibody (500-P247-50UG) in WB
Western Blot: To detect Human IFN-lambda2 by Western Blot analysis IL-28A Polyclonal Antibody (Product # 500-P247-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-lambda2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P247-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IFN-lambda2
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: PVARLHGALP DARGCHIAQF KSLSPQELQA FKRAKDALEE SLLLKDCRCH SRLFPRTWDL RQLQVRERPM ALEAELALTL KVLEATADTD PALVDVLDQP LHTLHHILSQ FRACIQPQPT AGPRTRGRLH HWLYRLQEAP KKESPGCLEA SVTFNLFRLL TRDLNCVASG DLCV.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IFN-lambda2. Anti-Human IFN-lambda2-specific antibody was purified by affinity chromatography employing an immobilized Human IFN-lambda2 matrix.
Sandwich ELISA: To detect Human IFN-lambda2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IFN-lambda2 (500-P247Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IFN-lambda2.
Western Blot: To detect Human IFN-lambda2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-lambda2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL-28A belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), and are also known as the type III Interferons.The IFN lambdas signal through a heterodimeric receptor of which one subunit, IL-10R2, is shared with other members of the superfamily. The second subunit, IFN lambda R1 or IL-28R alpha, is unique to the IFN lambdas. Signaling occurs through the Jak/STAT pathway in a similar manner as the type I IFN (IFN alpha/beta) and activates many of the same genes despite low sequence homology between the cytokines and receptors in the two families. Both IFN families display antiviral activity through the induction of antiviral protein production in target cells and the upregulation of MHC class I expression. These proteins also exhibit antiproliferative and antitumor effects, making them a possible alternative to IFN alpha cancer therapies. Unlike the type I IFN, which are able to stimulate most cells, response to IFN lambda stimulation appears to be limited to dendritic and some tumor cells due to the limited expression of IFN lambda R1. Another notable difference is the ability of the IFN lambda stimulation to drive dendritic cells towards the production of CD4+CD25+FoxP3+ regulatory T cells, suggesting a possible immunoregulatory role
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; Cytokine Zcyto20; IFN-lambda-2; IFN-lambda2; cytokine; IL-28A; ILN; Interferon lambda-2; Interleukin; interleukin 28A (interferon, lambda 2); Interleukin-28A
Gene Aliases: IFNL2; IFNL2a; IFNL3a; IL-28A; IL28A; ZCYTO20
UniProt ID: (Human) Q8IZJ0
Entrez Gene ID: (Human) 282616
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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