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Invitrogen
CTAGE5 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
CTAGE5 Antibody (500-P255BT-25UG) in WB
Western Blot: To detect Human MIA-2 by Western Blot analysis CTAGE5 Polyclonal Antibody, Biotin (Product # 500-P255BT-500UG) can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human MIA-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P255BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MIA-2
Conjugate
Biotin
Biotin
Biotin
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MLESTKLLAD LKKCGDLECE ALINRVSAMR DYRGPDCRYL NFTKGEEISV YVKLAGERED LWAGSKGKEF GYFPRDAVQI EEVFISEEIQ MSTKESDFLC L.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIA-2. Anti-Human MIA-2-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human MIA-2 by sandwich ELISA (using 100 mL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Human MIA-2 (500-P255) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIA-2.
Western Blot: To detect Human MIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human MIA-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P255BT-1MG will be provided as 2 x 500 µg
Target Information
CTAGE5/MIA-2 is a secreted cytokine, and a member of the MIA/OTOR family. Members of this family, which also includes MIA, OTOR, and TANGO, share a Src homology-3 (SH3)-like domain. MIA-2 is predominantly expressed in hepatocytes. Elevated levels of MIA-2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTAGE family member 5, ER export factor; CTAGE family, member 5; cutaneous T-cell lymphoma-associated antigen 5; melanoma inhibitory activity 2; meningioma expressed antigen 6 (coiled-coil proline-rich); meningioma-expressed antigen 6; meningioma-expressed antigen 6/11; MIA protein 2; MIA2; protein cTAGE-5; TANGO1-like; unnamed protein product
Gene Aliases: CTAGE5; MEA6; MGEA; MGEA11; MGEA6; TALI
UniProt ID: (Human) Q96PC5
Entrez Gene ID: (Human) 4253
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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