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Invitrogen
SMAD3 Polyclonal Antibody
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FIGURE: 1 / 79
SMAD3 Antibody (51-1500) in ICC/IF
Immunofluorescence analysis of SMAD3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SMAD3 Rabbit polyclonal Antibody (Product # 51-1500) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at ... View More
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20240507?time=20240507 "SMAD3 Antibody (51-1500) in WB")
.jpg?time=20240507?time=20240507 "SMAD3 Antibody (51-1500) in WB")
.jpg?time=20240507?time=20240507 "SMAD3 Antibody (51-1500) in ChIP, EMSA")
Product Details
51-1500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Deer,
Dog,
Human,
Luciferase,
Mink,
Mouse,
Non-human primate,
Rat,
Tag,
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 20 amino acid synthetic peptide derived from a central portion of the linker domain of human Smad3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZP586N0721; DKFZp686J10186; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; MGC60396; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD, mothers against DPP homolog 3; unnamed protein product
Gene Aliases: hMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; mad3; MADH3
UniProt ID: (Human) P84022
Entrez Gene ID: (Human) 4088
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding
transcription corepressor binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
transforming growth factor beta receptor binding
protein binding
collagen binding
beta-catenin binding
zinc ion binding
ligand-dependent nuclear receptor binding
DEAD/H-box RNA helicase binding
enzyme binding
protein kinase binding
phosphatase binding
chromatin DNA binding
ubiquitin protein ligase binding
mineralocorticoid receptor binding
sterol response element binding
glucocorticoid receptor binding
identical protein binding
protein homodimerization activity
ubiquitin binding
bHLH transcription factor binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
co-SMAD binding
I-SMAD binding
R-SMAD binding
DNA-binding transcription factor binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
osteoblast differentiation
ureteric bud development
response to hypoxia
release of cytochrome c from mitochondria
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
immune response
transforming growth factor beta receptor signaling pathway
JNK cascade
negative regulation of cell proliferation
anatomical structure morphogenesis
response to gamma radiation
gene expression
positive regulation of gene expression
negative regulation of gene expression
positive regulation of epithelial to mesenchymal transition
signal transduction involved in regulation of gene expression
cell differentiation
negative regulation of ossification
negative regulation of cell growth
adrenal gland development
positive regulation of cell migration
positive regulation of bone mineralization
primary miRNA processing
negative regulation of lipopolysaccharide-mediated signaling pathway
positive regulation of chondrocyte differentiation
developmental process
positive regulation of interleukin-1 beta production
regulation of transforming growth factor beta2 production
positive regulation of transforming growth factor beta3 production
activin receptor signaling pathway
negative regulation of osteoblast proliferation
cellular response to platelet-derived growth factor stimulus
nodal signaling pathway
wound healing
T cell activation
negative regulation of protein catabolic process
response to cocaine
positive regulation of protein import into nucleus
negative regulation of apoptotic process
cell-cell junction organization
positive regulation of nitric oxide biosynthetic process
negative regulation of cell differentiation
negative regulation of fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
regulation of immune response
protein stabilization
positive regulation of positive chemotaxis
negative regulation of cytosolic calcium ion concentration
positive regulation of stress fiber assembly
regulation of mitochondrial membrane potential
positive regulation of focal adhesion assembly
transdifferentiation
positive regulation of SMAD protein import into nucleus
SMAD protein signal transduction
regulation of dendritic spine morphogenesis
negative regulation of wound healing
trophoblast cell migration
negative regulation of lung blood pressure
cellular response to glucose stimulus
cellular response to growth factor stimulus
cellular response to transforming growth factor beta stimulus
negative regulation of transforming growth factor beta production
positive regulation of canonical Wnt signaling pathway
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
response to alcohol
cellular response to virus
transforming growth factor beta receptor superfamily signaling pathway
positive regulation of extracellular matrix assembly
regulation of miRNA transcription
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
negative regulation of cardiac muscle hypertrophy in response to stress
response to angiotensin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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