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Invitrogen
ALK Polyclonal Antibody
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FIGURE: 1 / 26
ALK Antibody (51-3900) in IHC (P)
Immunohistochemistry analysis of ALK showing staining in the cytoplasm of paraffin-embedded human skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperat... View More
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.jpg?time=20240507?time=20240507 "ALK Antibody (51-3900) in WB")
Product Details
51-3900
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A recombinant protein derived from amino acids 1359-1466 of the 1620 amino acid human ALK protein which corresponds to amino acids 426-528 of the 680 NPM-ALK chimeric protein.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Anaplastic Lymphoma Receptor Tyrosine Kinase (ALK) belongs to the insulin receptor superfamily. It is vital for brain development. Mutations, rearrangements, and amplifications in the ALK gene have been found in tumors, including anaplastic large cell lymphoma, neurblastoma, and non-small cell lung cancer. Chromosomal rearrangement is the most common genetic alteration. The translocation creates a fusion gene consisting of the ALK gene and the nucleophosmin gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ALK tyrosine kinase receptor; ALK/NPM1 fusion gene; Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; Anaplastic Lymphoma Kinase p80; anaplastic lymphoma receptor tyrosine kinase; CD246; CD246 antigen; EC 2.7.10.1; kinase ALK; mutant anaplastic lymphoma kinase; npm-alk; p80; TFG/ALK; unnamed protein product
Gene Aliases: ALK; ALK1; CD246; NBLST3
UniProt ID: (Human) Q9UM73
Entrez Gene ID: (Human) 238
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
protein binding
ATP binding
heparin binding
kinase activity
transferase activity
receptor signaling protein tyrosine kinase activator activity
identical protein binding
response to stress
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
nervous system development
brain development
phosphorylation
hippocampus development
adult behavior
swimming behavior
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
regulation of apoptotic process
regulation of neuron differentiation
protein autophosphorylation
neuron development
negative regulation of lipid catabolic process
positive regulation of NF-kappaB transcription factor activity
regulation of dopamine receptor signaling pathway
regulation of primary metabolic process
response to environmental enrichment
energy homeostasis
positive regulation of dendrite development
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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