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CD157 Monoclonal Antibody (eBioSY11B5 (SY11B5)), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 5
CD157 Antibody (53-1579-42) in Flow
Normal human peripheral blood cells were stained with CD14 Monoclonal Antibody, APC (Product # 17-0149-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (left) or CD157 Monoclonal Antibody, Alexa Fluor 488 (right). Cells in the monocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
53-1579-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioSY11B5 (SY11B5)
Conjugate
Alexa Fluor™ 488
Alexa Fluor™ 488
Alexa Fluor™ 488
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioSY11B5 monoclonal antibody recognizes human CD157 (Mo5, BST-1). CD157 is a 42-45 kDa, GPI-anchored protein with structural and functional similarities with CD38. CD157 was initially cloned because of its expression on monocytes and macrophages, and was subsequently discovered to be the same protein named BST-1, discovered for its expression on bone marrow stromal cells and its ability to stimulate the proliferation of a mouse pre-B cell line. CD157 is a pleiotropic ectoenzyme and is thought to act independently as an enzyme and receptor. Similar to CD38, CD157 is involved in the metabolism of NAD+ and this activity may be involved in regulating intracellular Ca2+ levels. As a receptor, upon binding of its putative ligand, CD157 is thought to initiate a signal transduction cascade resulting in the phosphorylation of cytoplasmic proteins including focal adhesion kinase (FAK). The mechanism and functional significance of CD157-initiated signal transduction remain to be fully characterized.
Applications Reported: This eBioSY11B5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioSY11B5 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
CD157, also known as Bone Marrow Stromal Cell Antigen 1 (BST1), is a pleiotropic ectoenzyme and glycosylphosphatidylinositol-anchored molecule that functions both as an enzyme and a receptor. Similar to CD38, CD157 is involved in the metabolism of NAD+, which may play a role in regulating intracellular Ca2+ levels. As an enzyme, CD157 is also referred to as ADP-ribosyl cyclase 2 and DAMP-1, and it facilitates pre-B cell growth. As a receptor, CD157 is thought to initiate a signal transduction cascade upon binding to its putative ligand, leading to the phosphorylation of cytoplasmic proteins, including focal adhesion kinase (FAK). While the mechanism and functional significance of CD157-initiated signal transduction are not fully characterized, its role in cellular signaling is evident. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis, suggesting a link to polyclonal B cell abnormalities observed in the disease. This overexpression in the stromal cell population may contribute to the pathophysiology of rheumatoid arthritis, highlighting the importance of CD157/BST1 in both normal immune function and disease states.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADP-ribosyl cyclase 2; bone marrow stromal antigen 1; cADPr hydrolase 2; CD157 antigen; cyclic ADP-ribose hydrolase 2; NAD(+) nucleosidase; NAD+ nucleosidase; unnamed protein product
Gene Aliases: cADPR2; CD157
UniProt ID: (Human) Q10588
Entrez Gene ID: (Human) 683
regulation of cell-matrix adhesion
regulation of cellular extravasation
humoral immune response
signal transduction
positive regulation of cell proliferation
regulation of actin cytoskeleton organization
regulation of inflammatory response
regulation of calcium-mediated signaling
regulation of neutrophil chemotaxis
regulation of superoxide metabolic process
regulation of integrin-mediated signaling pathway
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