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Invitrogen

FOXP3 Monoclonal Antibody (150D/E4), Alexa Fluor™ 488, eBioscience™

23 Published Figures
14 References
View all (154) FOXP3 antibodies

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Product Details

53-4774-41

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.5 µg)/test
View 11 publications 11 publications

ChIP assay (ChIP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

150D/E4

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2043861

Product Specific Information

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human/primate and mouse/rat Foxp3 protein, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to Foxp3 available from eBioscience, which have been used significantly in scientific literature, include the anti-human Foxp3 PCH101 (Product # 72-5776) and ebio7979 (Product # 12-7979), and the anti-mouse/rat Foxp3 FJK-16s (Product # 72-5775). The 150D/E4 has been mapped to the splice region in Exon 2 found in human cells. The splicing of this region has not been shown to occur in mouse. Characterization of this antibody to the splice variant has been limited to epitope mapping. It should be noted that other antibodies such as PCH101 and 236A/E7 will recognize both the spliced and full length forms of the Foxp3 protein and stain more intensely than 150D/E4 and FJK-16s in human cells.

The 150D/E4 antibody reacts with mouse/rat/human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of FoxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Applications Reported: This 150D/E4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 150D/E4 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The staining protocol has been optimized with freshly Ficoll-prepared PBMC or mouse splenocytes. The use of lysed whole blood is not recommended.

It is critical that this antibody be used in conjunction with the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) for flow cytometric analysis.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: forkhead box P3; forkhead box P3 transcript variant; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; JM2; MGC141961; MGC141963; RP23-54C14.1; scurfy; unnamed protein product

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Gene Aliases: AIID; DIETER; IPEX; JM2; PIDX; RGD1562112; scurfin; sf; XPID

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UniProt ID: (Human) Q9BZS1, (Mouse) Q99JB6

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Entrez Gene ID: (Human) 50943, (Mouse) 20371, (Rat) 317382

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription cofactor activity transcription corepressor activity protein binding histone acetyltransferase binding identical protein binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding NF-kappaB binding NFAT protein binding sequence-specific double-stranded DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter response to reactive oxygen species negative regulation of cytokine production CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment regulation of T cell anergy negative regulation of T cell cytokine production chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter negative regulation of cell proliferation response to virus establishment of endothelial blood-brain barrier response to lipopolysaccharide negative regulation of interferon-gamma production negative regulation of interleukin-10 production negative regulation of interleukin-17 production negative regulation of interleukin-2 production negative regulation of interleukin-4 production positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation T cell activation negative regulation of T cell proliferation T cell homeostasis regulatory T cell differentiation positive regulation of regulatory T cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of activated T cell proliferation negative regulation of defense response to virus negative regulation of immune response response to rapamycin negative regulation of T-helper 17 cell differentiation B cell homeostasis myeloid cell homeostasis T cell mediated immunity tolerance induction tolerance induction to self antigen regulation of immunoglobulin production positive regulation of T cell tolerance induction positive regulation of T cell anergy negative regulation of chronic inflammatory response positive regulation of peripheral T cell tolerance induction gene expression positive regulation of gene expression negative regulation of gene expression negative regulation of interleukin-5 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of interleukin-4 production positive regulation of transforming growth factor beta1 production positive regulation of immature T cell proliferation in thymus negative regulation of isotype switching to IgE isotypes regulation of isotype switching to IgG isotypes negative regulation of lymphocyte proliferation negative regulation of inflammatory response T cell receptor signaling pathway CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation
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