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CD169 (Siglec-1) Monoclonal Antibody (SER-4), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 3
CD169 (Siglec-1) Antibody (53-5755-82) in Flow
C57BL/6 mouse bone marrow cells were stained with Ly-6G/ Ly-6C Monoclonal Antibody, APC (Product # 17-5931-82) and 1.0 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4321-80) (left) or 1.0 µg of CD169 Monoclonal Antibody, Alexa Fluor 488 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
53-5755-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
SER-4
Conjugate
Alexa Fluor™ 488
Alexa Fluor™ 488
Alexa Fluor™ 488
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The SER-4 monoclonal antibody reacts with mouse CD169, which is also known as Sialoadhesin and Siglec-1. CD169 is a 185-kDa member of the Siglec family of adhesion and endocytic receptors that recognize sialylated glycans. This receptor is expressed on a subset of macrophages that are found with high frequency in the spleen, liver, lymph node, and bone marrow. CD169 can also be detected on monocytes in the bone marrow and periphery. This receptor plays a role in cell-cell adhesion, as well as internalization of sialylated virus and bacteria. This endocytic pathway allows CD169+ macrophages to present lipid antigens to iNKT cells, thereby leading to their activation. Studies have also demonstrated direct interaction of CD169 with regulatory T cells inhibits their proliferation.
The SER-4 antibody has been reported to have antagonist activity.
Applications Reported: This SER-4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This SER-4 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: SER; sheep erythrocyte receptor; sialic acid-binding Ig-like lectin 1
Gene Aliases: Cd169; Siglec-1; Sn
UniProt ID: (Mouse) Q62230
Entrez Gene ID: (Mouse) 20612
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