Invitrogen

Pan Cytokeratin Monoclonal Antibody (AE1/AE3), Alexa Fluor™ 488, eBioscience™

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14 Published Figures

82 References

View all (75) Cytokeratin Pan Type I/II antibodies

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Additional Information:

Pan Cytokeratin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 25

Pan Cytokeratin Antibody (53-9003-80) in ICC/IF

Immunocytochemistry of fixed and permeabilized MCF-7 cells using 1 µg/mL of Mouse IgG1 K Isotype Control Alexa Fluor® 488 (left) or 1 µg/mL of Anti-Pan Cytokeratin (AE1/AE3) Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Pan Cytokeratin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Pan Cytokeratin Antibody in Western Blot (WB)

Pan Cytokeratin Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

Pan Cytokeratin Antibody in Immunohistochemistry (IHC)

Pan Cytokeratin Antibody in Flow Cytometry (Flow)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Product Details

53-9003-80

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 27 publications 27 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 7 publications 7 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1 µg/mL

View 28 publications 28 publications

Flow Cytometry (Flow)

View 13 publications 13 publications

Proximity Ligation Assay (PLA) (PLA)

View 1 publication 1 publication

Inhibition Assays (Inhibition)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rabbit, Rhesus monkey

Published species

Amphibian, Dog, Human, Mouse, Non-human primate, Pig, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AE1/AE3

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Ambient (domestic); Wet ice (international)

AB_1834351

Product Specific Information

Description: The monoclonal antibodies AE1 and AE3 recognize many of the acidic and basic cytokeratin family members. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these proteins and their expression can help identify the origin of a neoplasm.

The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins also known as cytokeratin 1, 2, 3, 4, 5, 6, 7, and 8 while the AE1 antibody recognizes 56.5, 54', 50, 50', 48, and 40 kDa proteins (also known as CK10, 14, 15, 16 and 19). These antibodies can be used on a wide array of tissue samples from mouse, human, rat, primates (cynomolgus and rhesus), dog, cat, rabbit, and chicken.

Applications Reported: This AE1/AE3 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This AE1/AE3 antibody has been tested by immunofluorescent staining of formaldehyde-fixed and permeabilized or methanol-fixed MCF7 cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 40-kDa keratin intermediate filament; 47 kDa cytokeratin; 56 kDa cytokeratin; 57kd keratin; 57kDa keratin; 58 kDa cytokeratin; 60-kDa keratin; 65 kDa cytokeratin; 67 kDa cytokeratin; 70 kd type II; cell proliferation-inducing gene 46 protein; CK-1; CK-10; CK-13; CK-15; CK-17; CK-19; CK-1B; CK-2e; CK-2P; CK-3; CK-4; CK-5; CK-7; CK-8; cytokeratin 1; cytokeratin 10; cytokeratin 13; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 2; cytokeratin 3; cytokeratin 4; cytokeratin 5; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; Cytokeratin 7; cytokeratin 8; cytokeratin endo A; cytokeratin endo B; Cytokeratin-1; cytokeratin-10; cytokeratin-15; cytokeratin-16; cytokeratin-17; cytokeratin-18; cytokeratin-1B; Cytokeratin-2e; cytokeratin-2P; cytokeratin-3; cytokeratin-4; cytokeratin-5; cytokeratin-6A; cytokeratin-7; cytokeratin-8; cytoplasmic intermediate filament protein; cytoskeletal 57 kDa keratin; cytoskeletal protein; embryonic type II keratin 1; EndoA cytokeratin (5' end put.); putative; EndoA' cytokeratin (5' end put.); putative; epidermal keratin 10; epidermal keratin type I; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; epithelial keratin 1; epithelial keratin 10; epithelial keratin 2e; epithelial keratin-2e; focal non-epidermolytic palmoplantar keratoderma; hair alpha protein; HLA-DRB1-restricted epitope S1; HLA-DRB1-restricted epitope S2; HLA-DRB1-restricted epitope S3; HLA-DRB1-restricted epitope S4; HMWCK; intermediate filament protein; intermediate filament protein K16; intermediate filament protein; K7; K1; K1B; K2e; K2P; K3 keratin; K6-alpha; K76; K77; keratin 1 (epidermolytic hyperkeratosis); keratin 1, type II; keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 13; keratin 13, type I; keratin 14, type I; keratin 15, type I; keratin 16 (focal non-epidermolytic palmoplantar keratoderma); keratin 16 homolog; keratin 16, type I; keratin 17, type I; keratin 18, type I; keratin 19, type I; Keratin 1B; keratin 2 epidermis; keratin 2, type II; keratin 2A (epidermal ichthyosis bullosa of Siemens); keratin 2p; keratin 3, type II; keratin 4, type II; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6A, , type II; keratin 6A, type II; keratin 7, type II; keratin 76, type II; keratin 77; keratin 77, type II; keratin 8, type II; keratin 86; keratin complex 1, acidic, gene 10; keratin complex 1, acidic, gene 13; keratin complex 1, acidic, gene 14; keratin complex 1, acidic, gene 15; keratin complex 1, acidic, gene 16; keratin complex 1, acidic, gene 17; keratin complex 1, acidic, gene 18; keratin complex 1, acidic, gene 19; keratin complex 2, basic, gene 1; keratin complex 2, basic, gene 17; keratin complex 2, basic, gene 39; keratin complex 2, basic, gene 4; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6c; keratin complex 2, basic, gene 7; keratin complex 2, basic, gene 8; keratin complex 2, gene 17; keratin D; keratin intermediate filament 16a; keratin intermediate filament 16b; keratin K3; keratin K5; keratin type II; Keratin type II cytoskeletal 5; keratin, 55K type II cytoskeletal; keratin, epidermal type II, K6A; keratin, simple epithelial type I, K7; keratin, type I cytoskeletal 59 kDa; keratin, type I, 40-kd; keratin-10; keratin-15, basic; keratin-15, beta; keratin-2 epidermis; keratin-2e; keratin-6-alpha; KRT1B; KRT5; masticatory epithelia keratin 2p; mK6-alpha; ORF; Pan CK; pan Cytokeratin; pan keratin; pankeratin; PIG46; sarcolectin; suprabasal cytokeratin 10; tuftelin interacting protein 8; type I cytoskeletal 15; type II cytoskeletal 1b; type II keratin Kb39; type II keratin subunit protein; type II mesothelial keratin K7; type-II intermediate filament protein; type-II keratin Kb1; type-II keratin Kb2; Type-II keratin Kb3; Type-II Keratin Kb39; type-II keratin Kb4; type-II keratin Kb5; type-II keratin Kb6; type-II keratin Kb7; type-II keratin Kb8; type-II keratin Kb9; unnamed protein product

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Gene Aliases: 2310001L23Rik; 2310016L08Rik; 3300001P10Rik; 39.1; 4732484G22Rik; AEI2; BCIE; BIE; CARD2; CK-10; CK-14; CK-16; CK-17; CK-18; CK-19; CK-1B; CK-2e; CK-3; CK-4; CK-6A; CK-6C; CK-6E; CK-8; CK1; CK10; CK13; CK14; CK15; CK16; CK18; CK19; CK3; CK4; CK5; CK6A; CK6C; CK6D; CK7; CK8; CYK18; CYK4; CYK8; D130054E02Rik; D15Wsu77e; DDD; DDD1; EBS1; EBS1A; EBS1B; EBS1C; EBS1D; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; EBS3; EBS4; EGK_03684; EGK_03685; EHK; EHK1; EHK2; EHK2A; EHK2B; EndoA; EndoC; EPPK; FNEPPK; HUMCYT2A; IHL; K1; K10; K13; K14; K15; K16; K17; K18; K19; K1B; K1C1; K1CO; K1CP; K1CS; K1emb; K2C7; K2C8; K2E; K3; K4; K5; K6[a]; K6A; K6C; K6D; K7; K76; K77; K8; KER1; Ker10; KO; KPP; Krt-1.10; Krt-1.13; Krt-1.14; Krt-1.19; Krt-2.1; Krt-2.4; Krt-2.8; Krt1-10; Krt1-13; Krt1-14; Krt1-15; Krt1-16; Krt1-17; Krt1-18; Krt1-19; KRT13; KRT16A; KRT1A; KRT1B; Krt2-1; Krt2-17; Krt2-2; Krt2-4; Krt2-5; Krt2-6a; Krt2-6c; Krt2-7; Krt2-8; Krt239; KRT2A; KRT2B; KRT2E; KRT2P; KRT5A; KRT6C; KRT6D; KRT77; Krt86; KRTE; MECD2; mK1b; mK6[a]; MK6a; NEPPK; NFJ; PC1; PC2; PC3; PCHC1; SCL; Tfip8; TROMA-1; WSN1; WSN2

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Entrez Gene ID: (Dog) 444857, (Rhesus monkey) 699320, (Rabbit) 100353415, (Human) 3848, (Rabbit) 100009029, (Dog) 491006, (Human) 3858, (Rhesus monkey) 702153, (Human) 3860, (Rhesus monkey) 706830, (Dog) 490983, (Human) 3861, (Rhesus monkey) 100426180, (Dog) 100685323, (Rabbit) 100341120, (Rhesus monkey) 106994041, (Dog) 100685404, (Human) 3866, (Dog) 490978, (Rhesus monkey) 707236, (Human) 3868, (Rabbit) 100341376, (Rhesus monkey) 100426330, (Dog) 490977, (Human) 3872, (Rabbit) 100344942, (Dog) 477601, (Human) 3875, (Rabbit) 100354174, (Human) 3880, (Dog) 100685481, (Rabbit) 100353162, (Rhesus monkey) 699567, (Dog) 444850, (Human) 3849, (Dog) 100683401, (Rabbit) 100353668, (Rhesus monkey) 100425096, (Human) 3850, (Dog) 100682832, (Rhesus monkey) 700060, (Human) 3851, (Rabbit) 100348388, (Rhesus monkey) 100424400, (Rabbit) 100352658, (Dog) 100687760, (Human) 3852, (Dog) 486523, (Human) 3853, (Human) 3855, (Dog) 477602, (Rabbit) 100345579, (Rhesus monkey) 697425, (Rhesus monkey) 699194, (Dog) 486518, (Human) 51350, (Human) 374454, (Rabbit) 103348273, (Dog) 100683333, (Rabbit) 100348891, (Dog) 486513, (Rhesus monkey) 700449, (Human) 3856, (Mouse) 16678, (Mouse) 16661, (Mouse) 16663, (Mouse) 16664, (Mouse) 16665, (Mouse) 16666, (Mouse) 16667, (Mouse) 16668, (Mouse) 16669, (Mouse) 16681, (Mouse) 16682, (Mouse) 110308, (Mouse) 16687, (Mouse) 110310, (Mouse) 77055, (Mouse) 406220, (Mouse) 16691, (Rabbit) 100344183, (Rhesus monkey) 718983

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Function(s)

protein heterodimerization activity protein binding carbohydrate binding structural constituent of epidermis signaling receptor activity structural molecule activity structural constituent of cytoskeleton keratin filament binding scaffold protein binding RNA binding structural constituent of muscle cadherin binding involved in cell-cell adhesion macromolecular complex binding cytoskeletal protein binding

Process(es)

protein heterotetramerization morphogenesis of an epithelium cytoskeleton organization epithelial cell differentiation regulation of translation in response to stress intermediate filament organization keratinization wound healing negative regulation of keratinocyte differentiation keratinocyte development epidermis development keratinocyte differentiation positive regulation of epidermis development cornification cellular response to calcium ion tongue morphogenesis cellular response to retinoic acid Notch signaling pathway response to estrogen sarcomere organization cell differentiation involved in embryonic placenta development response to mechanical stimulus regulation of cell migration regulation of protein localization intermediate filament polymerization hair cycle intermediate filament bundle assembly positive regulation of keratinocyte differentiation positive regulation of cell growth hair follicle morphogenesis positive regulation of translation positive regulation of hair follicle development response to radiation positive regulation of cell proliferation cell differentiation negative regulation of cell migration killing of cells of other organism defense response to Gram-positive bacterium antimicrobial humoral immune response mediated by antimicrobial peptide negative regulation of entry of bacterium into host cell negative regulation of epithelial cell proliferation Wnt signaling pathway tumor necrosis factor-mediated signaling pathway Golgi to plasma membrane protein transport negative regulation of apoptotic process intermediate filament cytoskeleton organization extrinsic apoptotic signaling pathway hepatocyte apoptotic process complement activation, lectin pathway negative regulation of inflammatory response establishment of skin barrier response to hydrostatic pressure response to other organism epithelium development negative regulation of hepatocyte apoptotic process anatomical structure morphogenesis cell-cell adhesion pigmentation sebaceous gland development peptide cross-linking inflammatory response innate immune response keratinocyte migration keratinocyte activation keratinocyte proliferation negative regulation of epithelial cell differentiation response to oxidative stress fibrinolysis regulation of angiogenesis

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Pan Cytokeratin Monoclonal Antibody (AE1/AE3), Alexa Fluor™ 488, eBioscience™

Pan Cytokeratin Antibody (53-9003-80) in ICC/IF

Product Details

53-9003-80

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Proximity Ligation Assay (PLA) (PLA)

Inhibition Assays (Inhibition)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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