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Blimp-1 Monoclonal Antibody (5E7), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 2
Blimp-1 Antibody (53-9850-80) in Flow
BALB/c mouse splenocytes were stimulated for 72 hours with LPS (Product # 00-4976-03) and surface stained with CD45R (B220) Monoclonal Antibody, APC (Product # 17-0452-82). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 0.25 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4321-80) (left) or 0.25 µg of Blimp-1 Monoclonal Antibody, Alexa Fluor 488 (right). Total viable cells were used for analysis, as determined by Fixable Viabil... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
53-9850-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
5E7
Conjugate
Alexa Fluor™ 488
Alexa Fluor™ 488
Alexa Fluor™ 488
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 5E7 monoclonal antibody recognizes mouse B lymphocyte-induced maturation protein-1 (Blimp-1), which is also known as Prdm1. Blimp-1 is a transcriptional repressor with five zinc finger domains. Blimp-1 is expressed by terminally differentiating B and T cells. Blimp-1 is also expressed by some dendritic cells, and some NK cells. This transcriptional repressor is required for B cell differentiation into plasma cells and serves as a critical antagonist of CD4+ follicular helper T cell differentiation. While Blimp-1 is not required for priming and development of T and B cell effector function, it is critical for effector cell terminal differentiation. Blimp-1 promotes effector molecule secretion while suppressing proliferation. Blimp-1 also acts as a transcriptional antagonist of Bcl-6 and inhibits genes that are related to memory cell differentiation.
Applications Reported: This 5E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 5E7 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols . This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Target Information
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B lymphocyte induced maturation protein; B lymphocyte-induced maturation protein 1; beta-interferon gene positive regulatory domain I-binding factor; BLIMP 1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; PR domain-containing protein 1; PRDI BF1; RP1-134E15.1; transcriptional repressor; binds specifically to the PRDI element in the promoter of the beta-interferon gene; drives the maturation of B-lymphocytes into Ig secreting cells; unnamed protein product
Gene Aliases: b2b1765Clo; Blimp-1; Blimp1; PRDI-BF1; ZNFPR1A1
UniProt ID: (Mouse) Q60636
Entrez Gene ID: (Mouse) 12142
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
histone deacetylase binding
sequence-specific DNA binding
histone methyltransferase binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
morphogenesis of a branching structure
kidney development
embryonic placenta development
maternal placenta development
heart valve development
cardiac septum development
ventricular septum development
regulation of transcription from RNA polymerase II promoter
germ cell development
post-embryonic development
gene expression
positive regulation of gene expression
negative regulation of gene expression
peptidyl-arginine methylation
negative regulation of B cell proliferation
negative regulation of lipopolysaccharide-mediated signaling pathway
regulation of natural killer cell differentiation
regulation of extrathymic T cell differentiation
aorta development
regulation of cell proliferation
eye photoreceptor cell development
cell fate commitment
positive regulation of B cell differentiation
artery morphogenesis
cellular developmental process
regulation of NK T cell differentiation
retinal bipolar neuron differentiation
intestinal epithelial cell development
trophoblast giant cell differentiation
coronary vasculature development
sebum secreting cell proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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