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Invitrogen

Blimp-1 Monoclonal Antibody (5E7), Alexa Fluor™ 488, eBioscience™

1 Published Figure
1 Reference
View all (28) Blimp-1 antibodies

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Product Details

53-9850-80

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

5E7

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra spectra

Form

Liquid

Concentration

0.5 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848452

Product Specific Information

Description: This 5E7 monoclonal antibody recognizes mouse B lymphocyte-induced maturation protein-1 (Blimp-1), which is also known as Prdm1. Blimp-1 is a transcriptional repressor with five zinc finger domains. Blimp-1 is expressed by terminally differentiating B and T cells. Blimp-1 is also expressed by some dendritic cells, and some NK cells. This transcriptional repressor is required for B cell differentiation into plasma cells and serves as a critical antagonist of CD4+ follicular helper T cell differentiation. While Blimp-1 is not required for priming and development of T and B cell effector function, it is critical for effector cell terminal differentiation. Blimp-1 promotes effector molecule secretion while suppressing proliferation. Blimp-1 also acts as a transcriptional antagonist of Bcl-6 and inhibits genes that are related to memory cell differentiation.

Applications Reported: This 5E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 5E7 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols . This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Target Information

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: B lymphocyte induced maturation protein; B lymphocyte-induced maturation protein 1; beta-interferon gene positive regulatory domain I-binding factor; BLIMP 1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; PR domain-containing protein 1; PRDI BF1; RP1-134E15.1; transcriptional repressor; binds specifically to the PRDI element in the promoter of the beta-interferon gene; drives the maturation of B-lymphocytes into Ig secreting cells; unnamed protein product

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Gene Aliases: b2b1765Clo; Blimp-1; Blimp1; PRDI-BF1; ZNFPR1A1

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UniProt ID: (Mouse) Q60636

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Entrez Gene ID: (Mouse) 12142

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding histone deacetylase binding sequence-specific DNA binding histone methyltransferase binding sequence-specific double-stranded DNA binding promoter-specific chromatin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter in utero embryonic development morphogenesis of a branching structure kidney development embryonic placenta development maternal placenta development heart valve development cardiac septum development ventricular septum development regulation of transcription from RNA polymerase II promoter germ cell development post-embryonic development gene expression positive regulation of gene expression negative regulation of gene expression peptidyl-arginine methylation negative regulation of B cell proliferation negative regulation of lipopolysaccharide-mediated signaling pathway regulation of natural killer cell differentiation regulation of extrathymic T cell differentiation aorta development regulation of cell proliferation eye photoreceptor cell development cell fate commitment positive regulation of B cell differentiation artery morphogenesis cellular developmental process regulation of NK T cell differentiation retinal bipolar neuron differentiation intestinal epithelial cell development trophoblast giant cell differentiation coronary vasculature development sebum secreting cell proliferation
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