Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads. The Protein A/G IgG Binding Buffer is a phosphate-based buffer containing EDTA, formulated for antibody purification using either Protein A or Protein G.
These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.
The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.
Shared features of all Thermo Scientific Pierce IgG Binding and Elution Buffers:
• Ready to use
—carefully formulated and prefiltered 1X solutions
• Three binding buffers
—optimized for Protein A, Protein G or Protein A/G
• Two elution buffer options
—acidic (pH 2.8) or high salt (near-neutral)
—elution buffers are appropriate for antibody-antigen affinity purificationProducts available for each antibody purification method:
• Protein A IgG Binding Buffer:
pH 8.0; phosphate-based; contains glycerol and EDTA
• Protein A/G IgG Binding Buffer:
pH 8.0; phosphate-based; contains EDTA
• Protein G IgG Binding Buffer:
pH 5.0; contains 0.02% sodium azide
• IgG Elution Buffer:
pH 2.8; amine-based
• Gentle Ag/Ab Binding Buffer:
pH 8.0; phosphate-free; contains 0.05% sodium azide
• Gentle Ag/Ab Elution Buffer:
pH 6.6; high salt (ionic strength)Related ProductsPierce™ IgG Elution Buffer
For Research Use Only. Not for use in diagnostic procedures.