Informational:Informational:Pair your qPCR Master Mix with ready to use TaqMan Gene Expression or Genotyping Assay. Use our helpful TaqMan Search Wizard to find the Assays for your target of interest. Learn more
Applied Biosystems™

TaqMan™ Fast Virus 1-Step Multiplex Master Mix for qPCR (No ROX)

Catalog number: 5555532
Applied Biosystems™

TaqMan™ Fast Virus 1-Step Multiplex Master Mix for qPCR (No ROX)

Catalog number: 5555532
Catalog Number
5555532
Unit Size
1 x 1 mL
Price (USD)
Price: 408.00
Online price: 405.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
5 x 1 mL
Price (USD)
Price: 1,930.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
1 x 10 mL
Price (USD)
Price: 3,720.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
55555321 x 1 mL
Price: 408.00
Online price: 405.65
Your price:
-
55555345 x 1 mL
Price: 1,930.00
Your price:
-
55555361 x 10 mL
Price: 3,720.00
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations

TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) is designed to enable dye flexibility for multiplexing up to four different RNA/DNA targets for high-throughput RT-qPCR. The 4X formulation enhances detection of both RNA and DNA viral pathogens, even in the presence of challenging PCR inhibitors. TaqMan Fast Virus master mix is used for sensitive pathogen detection in research samples as well as multiplex gene expression studies with low target input. It has a single-tube format for consistent handling and processing and is ideal for high-throughput workflows, delivering results in <30 minutes.

Request an evaluation sample ›

Benefits of TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) include:
Multiplexing—optimized to detect up to four RNA/DNA viral pathogen and gene expression targets
High sensitivity—4X master mix to amplify RNA and DNA targets at low target input levels with six logs of dynamic range
Tolerant to PCR inhibitors—formulated to work in the presence of common RT-qPCR inhibitors found in blood, stool, and other complex samples
Dye flexibility—the formulation without ROX dye enables use of other dyes in the channel previously used to measure ROX
Simplified application—one-tube, one-step master mix offers a single run profile with RNA and DNA and allows easy mix-and-match of targets on a plate
High efficiency—increased RT-qPCR speed on fast and standard instruments

Optimized for multiplexing
TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) has been validated for detection of up to four targets simultaneously. Viral pathogen research often includes complex reactions with multiplexed primers and probes and internal controls. To meet this need, we developed this master mix for higher order multiplex reactions with multiple gene expression targets (see figure below). To simplify your experiments, a single TaqMan Fast Virus master mix protocol has been developed to amplify both types of nucleic acid, so you can interrogate both RNA and DNA virus targets next to each other on the same plate using the same handling steps.

High sensitivity
Many viral pathogen research samples have very low levels of nucleic acid targets. TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) is optimized for highly sensitive detection of viral targets and low-titer pathogens (see figure). Formulated at a higher concentration, this master mix allows you to set up smaller reactions and run fast-cycling instruments and protocols while obtaining at least the same sensitivity as expected from standard-cycling qPCR. Alternatively, larger sample input amounts can be added to standard reaction volumes for more accurate quantification of low-titer samples.

Consistent results in the presence of inhibitors
Research samples assayed for viral pathogens commonly include materials such as blood, dirt, and tissues that can inhibit qPCR reactions. TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) includes buffer components and proprietary additives that are optimized to handle RT-qPCR inhibitors to help ensure consistent performance and provide confidence in your results. The master mix has been tested to enable robust performance in the presence of common inhibitors, including heparin, hematin, and humic acid (see figure).

Dye flexibility
TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) does not contain the ROX passive reference dye. This allows higher order multiplexing because other dyes, such as JUN (or similar emission wavelength dye) can be measured in the channel previously used to measure ROX. Because of this flexibility, TaqMan Fast Virus master mix can be used with common real-time PCR system and allows selection of the desired passive reference dye to best fit your needs.

Fast results
TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) speeds your time to results and streamlines the use of your real-time PCR instruments with a fast protocol. The high-concentration formulation allows for more sample containing the target nucleic acid to be added in the smaller reaction volumes required to run fast protocols. This enables you to maintain sensitivity with low-titer research samples while improving speed and throughput.

TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) provides robust performance, multiplexing capabilities in the presence of common RT-qPCR inhibitors, and convenient reaction setup allow you to have more confidence in your results.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Primer-probe
PCR Method
1-step RT-qPCR, Multiplex qPCR
Reaction Speed
Fast or Standard
For Use With (Equipment)
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System
Passive Reference Dye
None
Shipping Condition
Dry Ice
For Use With (Application)
Gene Expression, Virus Detection
Concentration
4X
Hot Start
Built-In Hot Start
No. of Reactions
200 x 20 μL Reactions
Sample Type
RNA, DNA
Volume
1 x 1 mL

Contents & Storage

Contains 1 x 1 mL tube of 4X RT-PCR master mix sufficient for 200 x 20 μL reactions.
The master mix contains:
• Hot-start AmpliTaq Fast DNA Polymerase
• Thermostable MMLV Reverse Transcriptase
• dNTPs
• RNase Inhibitor
• Optimized buffer

Store at -15°C to -25°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text