S6 Multispecies In-Cell ELISA Kit, Colorimetric

Catalog number: 62207

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Pierce S6 Colorimetric In-Cell ELISA Kit is a colorimetric microplate assay optimized to measure total and phosphorylated ribosomal S6 protein (phospho-S6) in whole cells.

Features of the S6 Colorimetric In-Cell ELISA Kit:

• Measures total and phosphorylated ribosomal S6 protein in whole cells using a 96- or 384-well microplate
• Colorimetric detection kit for use with standard microplate readers
• Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
• Provides better data and higher throughput than quantitative western blotting
• Less costly (one well vs. one lane) than western blotting

This ELISA kit for whole cells includes a mouse monoclonal anti-S6 antibody, rabbit monoclonal anti-phospho-(Ser235/236) S6 antibody, detection reagents and all buffers required for colorimetric detection. The S6 Colorimetric In-Cell ELISA Kit enables simultaneous detection of both total and phospho-(Ser235/236) S6.

Overview of S6 and phosphorylation of S6:
The ribosomal S6 protein is a component of the small subunit of ribsomes (40S) and is involved in the regulation of translation. The activity of S6 is regulated, in part, by the downstream effectors of mTOR, the ribosomal protein S6 kinase (S6K). Phosphorylation of S6 by S6K is important in the regulation of protein synthesis, cell size, cell-cycle progression, glucose homeostasis and survival of newborns. S6K activation and phosphorylation of S6 are implicated in translation efficiency of mRNAs with a 5'-terminal oligopyrimidine tract (TOP mRNAs) in certain physiological conditions. S6 phosphorylation occurs on 5 serine residues (Ser235, Ser236, Ser240, Ser244 and Ser247) within the C-terminus of the protein and are evolutionarily conserved in higher eukaryotes. The S6 Colorimetric In-Cell ELISA Kit enables simultaneous detection of both total and phospho-(Ser235/236) S6.

Related Products
Pierce™ S6 Near Infrared In-Cell ELISA Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Other Proteins
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
Detector Antibody Conjugate: Unconjugated (uses Secondary Antibody)
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: S6
Gene ID: 6194
Gene Name: ribosomal protein S6
Gene Symbol: RPS6
Immunoassay Kit Format: In-Cell ELISA Kit
Immunoassay Kit Grade: Colorimetric
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: Phosphoprotein NP33
Protein Name: 40S ribosomal protein S6
Target (Kit Named): S6
Target Species (Named): Multispecies
Target Species (Validated): Human
UniProt ID: P62753

Contents & storage

Sufficient For: 96 microplate wells of overnight-cultured cells
• Blocking Buffer, 50 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Detergent), 10 mL
• Surfact-Amps X-100 (10% Detergent), 10 mL
• HRP Conjugate, 0.14 mL
• TMB Substrate, 58 mL
• TMB Stop Solution, 55 mL
• Janus Green Whole-Cell Stain, 50 mL
• Elution Buffer, 2 x 30 mL
• Thin Plate Seal Assembly, 8 each
• Anti-Phospho S6 (Ser 235/236) Antibody
• Anti-S6 Antibody

Store antibodies as indicated on vial. Store remaining kit components at 4°C.

Documents

Manuals & protocols