S6 Multispecies In-Cell ELISA Kit, Near Infrared

Catalog number: 62212

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Pierce S6 Near Infrared In-Cell ELISA Kit is a fluorescent microplate assay optimized to measure total and phosphorylated ribosomal S6 protein (phospho-S6) in whole cells.

Features of the S6 Near Infrared In-Cell ELISA Kit:

• Near infrared fluorescent detection kit for use with IR imaging system
• Measures total and phosphorylated ribosomal S6 protein in whole cells using a 96- or 384-well microplate
• Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
• Provides better data and higher throughput than quantitative western blotting
• Less costly (one well vs. one lane) than western blotting

This ELISA kit for whole cells includes a mouse monoclonal anti-S6 antibody, rabbit monoclonal anti-phospho-(Ser235/236) S6 antibody, detection reagents and all buffers required for near infrared detection. The S6 Near Infrared In-Cell ELISA Kit enables simultaneous detection of both total and phospho-(Ser235/236) S6.

Overview of S6 and phosphorylation of S6:
The ribosomal S6 protein is a component of the small subunit of ribsomes (40S) and is involved in the regulation of translation. The activity of S6 is regulated, in part, by the downstream effectors of mTOR, the ribosomal protein S6 kinase (S6K). Phosphorylation of S6 by S6K is important in the regulation of protein synthesis, cell size, cell-cycle progression, glucose homeostasis and survival of newborns. S6K activation and phosphorylation of S6 are implicated in translation efficiency of mRNAs with a 5'-terminal oligopyrimidine tract (TOP mRNAs) in certain physiological conditions. S6 phosphorylation occurs on 5 serine residues (Ser235, Ser236, Ser240, Ser244 and Ser247) within the C-terminus of the protein and are evolutionarily conserved in higher eukaryotes. The S6 Near Infrared In-Cell ELISA Kit enables simultaneous detection of both total and phospho-(Ser235/236) S6.

Related Products
Pierce™ S6 Colorimetric In-Cell ELISA Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Other Proteins
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Fluorescent
Detector Antibody Conjugate: Unconjugated (uses Secondary Antibody)
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: S6
Gene ID: 6194
Gene Name: ribosomal protein S6
Gene Symbol: RPS6
Immunoassay Kit Format: In-Cell ELISA Kit
Immunoassay Kit Grade: Near Infrared
Label or Dye: DyLight™ 680, DyLight™ 800
Product Size: 96 tests
Protein Aliases: Phosphoprotein NP33
Protein Name: 40S ribosomal protein S6
Target (Kit Named): S6
Target Species (Named): Multispecies
Target Species (Validated): Human
UniProt ID: P62753

Contents & storage

Sufficient For: 96 microplate wells of overnight-cultured cells
• Blocking Buffer, 100 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Detergent), 10 mL
• Surfact-Amps X-100 (10% Detergent), 10 mL
• DyLight 680 Goat Anti-Mouse IgG (H+L), 55 µL
• DyLight 800 Goat Anti-Rabbit IgG (H+L), 55 µL

Store antibodies as indicated on vial. Store remaining kit components at 4°C.

Documents

Manuals & protocols