STAT3 Multispecies In-Cell ELISA Kit, Near Infrared

Catalog number:  62214

 Related applications: Protein Assays and Analysis

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Thermo Scientific Pierce STAT3 Near Infrared In-Cell ELISA Kit is a fluorescent microplate assays optimized to measure total and phosphorylated signal transducers and activators of transcription 3 (phospho-STAT3) in whole cells.

Features of STAT3 Near Infrared In-Cell ELISA Kit:

• Measures total and phosphorylated signal transducers and activators of transcription 3 (STAT3) in whole cells using a 96-well microplates
• Reliable near infrared fluorescent detection kit for use with IR imaging system is available
• Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
• Provides better data and higher throughput than quantitative Western blotting
• Less costly (one well vs. one lane) than Western blotting

STAT3 Near Infrared In-Cell ELISA Kit for whole cells include a mouse monoclonal anti-STAT3 antibody, rabbit monoclonal anti-phospho-(Tyr705) STAT3 antibody, detection reagents and all buffers required for either colorimetric or near infrared detection. The STAT3 In-Cell ELISA Kits enable simultaneous detection of both total and phospho-(Tyr705) STAT3.

Overview of STAT3 and phosphorylation of STAT3:
STAT3 belongs to the STAT family of transcription factors which regulate many aspects of cell growth, survival and differentiation. Inactive STAT proteins are located in the cytosol until phosphorylated by receptor associated kinases. Upon phosphorylation, STAT proteins form homodimers or heterodimers with other STAT proteins and translocate into the cell nucleus. STAT3 is activated in response to cytokines and growth factors such as interons, epidermal growth factors, interleukins and bone morphogenetic protein 2. STAT3 is known to be regulated by Rac1, PIAS3, JAK2 and c-MET. Activation of STAT3 is required for embyronic stem cell survial and development of mouse embryos past embyronic day 7. STAT3 is also implicated in human cancers.

Related Products
Pierce™ STAT3 Colorimetric In-Cell ELISA Kit
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Other Proteins
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Fluorescent
Detector Antibody Conjugate: Unconjugated (uses Secondary Antibody)
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene ID: 6774
Gene Name: signal transducer and activator of transcription 3
Gene Symbol: STAT3
Immunoassay Kit Format: In-Cell ELISA Kit
Immunoassay Kit Grade: Near Infrared
Label or Dye: DyLight™ 680, DyLight™ 800
Product Size: 96 tests
Protein Aliases: Acute-phase response factor
Protein Name: Signal transducer and activator of transcription 3
Target (Kit Named): STAT3
Target Species (Named): Multispecies
Target Species (Validated): Human
UniProt ID: P40763

Contents & storage

Sufficient For: 96 microplate wells of overnight-cultured cells
• Blocking Buffer, 100 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Detergent), 10 mL
• Surfact-Amps X-100 (10% Detergent), 10 mL
• DyLight 680 Goat Anti-Mouse IgG (H+L), 55 µL
• DyLight 800 Goat Anti-Rabbit IgG (H+L), 55 µL
• Thin Plate Seal Assembly, 8 each
• Anti-Phospho STAT3 (Y705) Antibody
• Anti-STAT3 Antibody

Store antibodies as indicated on vial. Store remaining components at 4°C.


Manuals & protocols