p53 Colorimetric In-Cell ELISA Kit, Multispecies

Catalog number: 62216

Thermo Scientific™  Related applications: Protein Assays and Analysis

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The Thermo Scientific Pierce p53 Colorimetric In-Cell ELISA Kit is a colorimetric microplate assay optimized to measure total tumor protein 53 (p53) and tubulin in whole cells. This ELISA kit includes a mouse monoclonal anti-p53 antibody, rabbit monoclonal anti-alpha-Tubulin antibody, detection reagents, and all buffers required for colorimetric detection.

Features of the p53 Colorimetric In-Cell ELISA Kit:

• Measures total tumor protein 53 (p53) and tubulin in whole cells using a 96- or 384-well microplates
• Colorimetric detection kit for use with standard microplate readers
• Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
• Provides better data and higher throughput than quantitative Western blotting
• Less costly (one well vs. one lane) than Western blotting

Related Products
Pierce™ p53 Near Infrared In-Cell ELISA Kit
For Research Use Only. Not for use in diagnostic procedures.


Species: Multiple
Product Size: 96 assays
Protein Family: Other Proteins
Detection Method: Colorimetric
Research Category: Signal Transduction

Contents & storage

Sufficient For: 96 microplate wells of overnight-cultured cells
• Blocking Buffer, 50 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Detergent), 10 mL
• Surfact-Amps X-100 (10% Detergent), 10 mL
• HRP Conjugate, 0.14 mL
• TMB Substrate, 58 mL
• TMB Stop Solution, 55 mL
• Janus Green Whole-Cell Stain, 50 mL
• Elution Buffer, 2 x 30 mL
• Thin Plate Seal Assembly, 8 each
• Anti-p53 Antibody
• Anti-alpha Tubulin Antibody

Store antibodies as indicated on vial. Store remaining components at 4°C.


Manuals & protocols