p53 Multispecies In-Cell ELISA Kit, Colorimetric

      p53 Multispecies In-Cell ELISA Kit, Colorimetric

      Catalog number:  62216

      Invitrogen™  Related applications: Protein Assays and Analysis

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      Description

      The Thermo Scientific Pierce p53 Colorimetric In-Cell ELISA Kit is a colorimetric microplate assay optimized to measure total tumor protein 53 (p53) and tubulin in whole cells. This ELISA kit includes a mouse monoclonal anti-p53 antibody, rabbit monoclonal anti-alpha-Tubulin antibody, detection reagents, and all buffers required for colorimetric detection.

      Features of the p53 Colorimetric In-Cell ELISA Kit:

      • Measures total tumor protein 53 (p53) and tubulin in whole cells using a 96- or 384-well microplates
      • Colorimetric detection kit for use with standard microplate readers
      • Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
      • Provides better data and higher throughput than quantitative Western blotting
      • Less costly (one well vs. one lane) than Western blotting

      Related Products
      Pierce™ p53 Near Infrared In-Cell ELISA Kit
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Protein Family: Other Proteins
      Assay Range: See product documentation
      Assay Sensitivity: See product documentation
      Detection Method: Colorimetric
      Detector Antibody Conjugate: Unconjugated (uses Secondary Antibody)
      For Use With (Equipment): Microplate Reader
      Former Identifiers: Pierce
      Gene Aliases: BCC7, LFS1, P53, TRP53
      Gene ID: 7157
      Gene Name: tumor protein p53
      Gene Symbol: TP53
      Immunoassay Kit Format: In-Cell ELISA Kit
      Immunoassay Kit Grade: Colorimetric
      Label or Dye: HRP (Horseradish Peroxidase)
      Product Size: 96 tests
      Protein Aliases: Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
      Protein Name: Cellular tumor antigen p53
      Target (Kit Named): p53
      Target Species (Named): Multispecies
      Target Species (Validated): Human
      UniProt ID: P04637

      Contents & storage

      Sufficient For: 96 microplate wells of overnight-cultured cells
      • Blocking Buffer, 50 mL
      • 20X Tris Buffered Saline, 50 mL
      • Surfact-Amps 20 (10% Detergent), 10 mL
      • Surfact-Amps X-100 (10% Detergent), 10 mL
      • HRP Conjugate, 0.14 mL
      • TMB Substrate, 58 mL
      • TMB Stop Solution, 55 mL
      • Janus Green Whole-Cell Stain, 50 mL
      • Elution Buffer, 2 x 30 mL
      • Thin Plate Seal Assembly, 8 each
      • Anti-p53 Antibody
      • Anti-alpha Tubulin Antibody

      Store antibodies as indicated on vial. Store remaining components at 4°C.

      Documents

      Manuals & protocols