PARP (Cleaved) Multispecies In-Cell ELISA Kit, Near Infrared

Catalog number: 62224

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Pierce Cleaved PARP Near Infrared In-Cell ELISA Kit is a near infrared microplate assay optimized to measure activated (cleaved) poly (ADP-ribose) polymerase (PARP) and tubulin in whole cells.

Features of the Cleaved PARP Near Infrared In-Cell ELISA Kit:

• Measures activated (cleaved) poly (ADP-ribose) polymerase (PARP) and tubulin in whole cells using 96-well or 384-well microplates
• Near infrared fluorescent detection kit for use with IR imaging system
• Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
• Provides better data and higher throughput than quantitative western blotting
• Less costly (one well vs. one lane) than western blotting

This ELISA kit for whole cells includes a rabbit monoclonal anti-(cleaved) PARP antibody, mouse monoclonal anti-alpha-Tubulin antibody, detection reagents and all buffers required for near infrared detection. The kit enables simultaneous detection of both Cleaved PARP and Tubulin as a reference.

Overview of PARP:
Poly (ADP-ribose) polymerase (PARP) plays a role in repair of single stranded DNA nicks. PARP also regulates proteins such as histones and telomerase through ADP-ribosylation and is an important marker of caspase 3-mediated apoptosis. In response to apoptotic signals, activated caspase 3 cleaves the 116 kDa PARP protein into 85 kDa and 25 kDa fragments. In humans, PARP cleavage occurs at Asp 214 and Gly 215 residues. Cleavage of PARP correlates with DNA fragmentation and other morphological changes making it a critical marker for apoptosis. The Cleaved PARP In-Cell ELISA Kit enables simultaneous detection of both Cleaved PARP and Tubulin as a reference.

Related Products
Pierce™ Cleaved PARP Colorimetric In-Cell ELISA Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Other Proteins
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Fluorescent
Detector Antibody Conjugate: Unconjugated (uses Secondary Antibody)
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1
Gene ID: 142
Gene Name: poly(ADP-ribose) polymerase 1
Gene Symbol: PARP1
Immunoassay Kit Format: In-Cell ELISA Kit
Immunoassay Kit Grade: Near Infrared
Label or Dye: DyLight™ 680, DyLight™ 800
Product Size: 96 tests
Protein Name: Poly [ADP-ribose] polymerase 1
Target (Kit Named): PARP
Target Species (Named): Multispecies
Target Species (Validated): Human
Target Sub-Specificity: Cleaved
UniProt ID: P09874

Contents & storage

Sufficient For: 96 microplate wells of overnight-cultured cells
• Blocking Buffer, 100 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Detergent), 10 mL
• Surfact-Amps X-100 (10% Detergent), 10 mL
• DyLight 680 Goat Anti-Mouse IgG (H+L), 55 µL
• DyLight 800 Goat Anti-Rabbit IgG (H+L), 55 µL
• Thin Plate Seal Assembly, 8 each
• Anti-Cleaved PARP Antibody
• Anti-alpha Tubulin Antibody

Store antibodies as indicated on vial. Store remaining components at 4°C.

Documents

Manuals & protocols